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WoS SCOPUS Document Type Document Title Abstract Authors Affiliation ResearcherID (WoS) AuthorsID (SCOPUS) Author Email(s) Journal Name JCR Abbreviation ISSN eISSN Volume Issue WoS Edition WoS Category JCR Year IF JCR (%) FWCI ⓘ FWCI Update Date WoS Citation SCOPUS Citation Keywords (WoS) KeywordsPlus (WoS) Keywords (SCOPUS) KeywordsPlus (SCOPUS) Language Publication Stage Publication Year Publication Date DOI JCR Link DOI Link WOS Link SCOPUS Link
○ ○ Article ⓘ A High-Yield Recombinant Inactivated Whole-Virion Nasal Influenza A(H1N1)pdm09 Virus Vaccine with an Attenuated PB2 Gene ⓘ During the 2009 H1N1 pandemic (pdm09), the poor replication of PR8-derived vaccine strains in embryonated chicken eggs (ECEs) delayed vaccine production, necessitating costly adjuvants. To improve egg-based yield, we generated PB2-substituted H1N1 strains via reverse genetics, replacing PR8 PB2 with a PB2 lacking mammalian-adaptive mutations (dtxPB2), cognate pdm09 PB2 (19PB2), or avian PB2. All PB2-substituted strains achieved over tenfold higher titers than the conventional PR8 PB2-containing strain (rGD19), with rGD19/dtxPB2 and rGD19/19PB2 exhibiting significantly higher titers and reduced murine virulence. Among these, rGD19/19PB2 produced the highest hemagglutinin (HA) yield and, when administered intranasally as a binary ethyleneimine (BEI)-inactivated whole-virion vaccine, elicited a significantly stronger broncho-alveolar IgA response than rGD19. Both rGD19 and rGD19/19PB2 provided comparable protection against a homologous H1N1 challenge, yet only rGD19/19PB2 conferred full survival protection after a lethal heterologous H3N2 challenge. These findings show that incorporation of cognate PB2 enhances H1N1 replication in ECEs and antigen yield, reduces murine virulence, and confers robust homo- and heterosubtypic protection via intranasal immunization, underscoring the promise of PB2-modified H1N1 strains as inactivated mucosal whole-virion vaccines for future vaccine development. ⓘ Son, Seung-Eun; Song, Jin-Ha; Kim, Ho-Won; An, Se-Hee; Kim, Seung-Ji; Lee, Chung-Young; Kwon, Hyuk-Joon; Choi, Kang-Seuk ⓘ Seoul Natl Univ, Coll Vet Med, Lab Avian Dis, Seoul 08826, South Korea; Anim & Plant Quarantine Agcy, Avian Influenza Res & Diagnost Div, Gimcheon Si 39660, South Korea; Kyungpook Natl Univ, Sch Med, Dept Microbiol, Daegu 41944, South Korea; Seoul Natl Univ, Res Inst Vet Sci, Coll Vet Med, Seoul 08826, South Korea; Seoul Natl Univ, Coll Vet Med, Dept Farm Anim Med, Lab Poultry Med, Seoul 88026, South Korea; Seoul Natl Univ, BK21 PLUS Vet Sci, Seoul 88026, South Korea; Seoul Natl Univ, Inst Green Bio Sci & Technol GBST, Farm Anim Clin Training & Res Ctr FACTRC, Pyeongchang 25354, South Korea; GeNiner Inc, Seoul 08826, South Korea ⓘ ⓘ 57221094613; 57221101773; 58516482000; 57195539685; 58516580700; 57195422071; 57196596247; 35798047900 ⓘ arbre04@snu.ac.kr; sjh1243@snu.ac.kr; iamkhw52@snu.ac.kr; ashpri@korea.kr; seungji910@snu.ac.kr; cylee87@knu.ac.kr; kwonhj01@snu.ac.kr; kchoi0608@snu.ac.kr; ⓘ INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES ⓘ INT J MOL SCI ⓘ 1661-6596 1422-0067 26 12 SCIE BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY ⓘ 2024 4.9 22.4 ⓘ 0 0 pandemic 2009 virus; cognate PB2; egg-propagated vaccine; high-yield; inactivated mucosal vaccine; heterosubtypic protection ⓘ SEASONAL INFLUENZA; VIRAL-INFECTION; A VIRUS; HEMAGGLUTININ; NEURAMINIDASE; REPLICATION; NEUTRALIZATION; GENERATION; PROTECTION; MUTATIONS ⓘ cognate PB2; egg-propagated vaccine; heterosubtypic protection; high-yield; inactivated mucosal vaccine; pandemic 2009 virus ⓘ ⓘ English 2025 2025-06-07 10.3390/ijms26125489 ⓘ 바로가기 바로가기 바로가기 바로가기
○ ○ Article ⓘ Calcium Enhances the Effectiveness of Melatonin in Improving Nutritional Properties of Soybean Sprouts and Germination Under Salt and Cadmium Stress ⓘ Salinity and cadmium exposure to agrarian land lowers crop yield and imposes toxicity in the food chain, ultimately affecting sustainable agriculture. Melatonin (Mel) and calcium (Ca) have been reported as potent regulators of plant growth and stress resistance. Based on this scenario, this study investigated the sole and combined effects of Mel and Ca on improving the antioxidant properties, mineral content, germination of sprout, and stress tolerance of soybean seedlings under salt and cadmium (Cd) stress. Optimal doses of 20 mu M Mel and 1 mM Ca were identified to enhance sprout quality and seed germination. Treatments with Mel > 20 mu M inhibited germination, while the combination of Mel (20 mu M) and Ca (1 mM) significantly improved germination, mineral content (Ca, P, K), and antioxidant properties, including DPPH(2,2-Diphenyl-1-picrylhydrazyl) activity, polyphenols, flavonoids, and superoxide dismutase (SOD) activity. However, melatonin > 50 mu M could completely cease the sprouting, whereas a Ca concentration of up to 10 mM was observed to be normal in sprouting. Additionally, this combination reduced malondialdehyde (MDA) levels and enhanced the proline, indicating decreased oxidative stress in soybean seedlings under stress conditions. Among various treatments tested, the Mel-Ca combination was most effective in enhancing sprout biomass, antioxidant activity, and seed viability under Salt+Cd stress. These findings underscore the synergistic role of Ca in optimizing melatonin pretreatment for stress mitigation in soybean seeds and also address the precaution for a possible negative impact of melatonin effects. ⓘ Adhikari, Arjun; Sapkota, Mahesh; Savidya, Raddella Nishani; Tosin, Ajayi Tolulope; Adam, Muchanji; Alam, Mohammad Naushad; Kwon, Eun-Hae; Kang, Sang-Mo; Shaffique, Shifa; Lee, In-Jung ⓘ Kyungpook Natl Univ, Dept Appl Biosci, Daegu 41566, South Korea ⓘ Lee, In-Jung/GLS-0432-2022; shaffique, shifa/KUC-7102-2024; Kang, Sang-Mo/MBG-7823-2025; Adhikari, Arjun/AAV-6297-2021 ⓘ 57195601415; 59557445100; 59556834100; 59557029300; 59556632800; 59557239900; 57224398710; 56189696900; 57203898867; 16425830900 ⓘ arjun@knu.ac.kr; sapkota@knu.ac.kr; nishanisrd@gmail.com; ajayitolu84@knu.ac.kr; adammuchanji@gmail.com; alam97ad@gmail.com; eunhae.kwon1@gmail.com; kmoya@hanmail.net; shifa.2021@knu.ac.kr; ijlee@knu.ac.kr; ⓘ INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES ⓘ INT J MOL SCI ⓘ 1661-6596 1422-0067 26 3 SCIE BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY ⓘ 2024 4.9 22.4 0 2025-05-07 ⓘ 0 0 abiotic stresses; antioxidants; mineral elements; nutrients; germination; sustainable agriculture; yield ⓘ TOTAL ANTIOXIDANT CAPACITY; VICIA-FABA L.; POTASSIUM; TOLERANCE; SEEDLINGS; TOXICITY; GROWTH ⓘ abiotic stresses; antioxidants; germination; mineral elements; nutrients; sustainable agriculture; yield ⓘ Antioxidants; Cadmium; Calcium; Germination; Glycine max; Malondialdehyde; Melatonin; Oxidative Stress; Salt Stress; Seedlings; Stress, Physiological; 1,1 diphenyl 2 picrylhydrazyl; cadmium; calcium; flavonoid; malonaldehyde; melatonin; mineral; phosphorus; polyphenol; potassium; proline; sodium chloride; superoxide dismutase; antioxidant; malonaldehyde; melatonin; antioxidant activity; Article; biomass; controlled study; DPPH radical scavenging assay; enzyme activity; food chain; germination; nonhuman; nutrient content; nutritional value; oxidative stress; plant growth; plant seed; root length; salinity; salt stress; seed viability; seedling; shoot length; soil pollution; soybean; sprout; sprouting; sustainable agriculture; drug effect; growth, development and aging; metabolism; physiological stress; salt stress; seedling ⓘ English 2025 2025-02 10.3390/ijms26030878 ⓘ 바로가기 바로가기 바로가기 바로가기
○ ○ Article ⓘ Changes in muscle oxygenation and activity during cumulative isometric muscle contraction: new insight into muscle fatigue ⓘ This study aimed to investigate the progression of muscle fatigue during submaximal efforts by examining alterations in muscle activation and oxygen saturation, employing surface electromyography ( S EMG) and near-infrared spectroscopy (NIRS) measurements. Participants performed intermittent voluntary isometric knee extension tasks at 50% of maximal voluntary contraction to induce muscle fatigue. This was conducted consecutively until they could no longer generate the target torque. Knee extension torque, S EMG, and NIRS data from the vastus lateralis were collected. Torque variability, the magnitude and frequency of the S EMG signal, and NIRS-derived parameters of the tissue oxygen saturation index (TSI) were analyzed. An increase in the magnitude (p < 0.001) and a decrease in the spectrum (p < 0.001) of the S EMG signal were observed, followed by a rise in torque variability (p < 0.001), despite the average magnitude of knee extension torque remaining constant across the trials. The NIRS measurements indicated alterations in TSI parameters, reflecting increased metabolic demand and diminished oxygen supply in the fatigued muscle (p < 0.001). Furthermore, significant interrelationships were found between changes in torque, S EMG, and NIRS variables due to the development of muscle fatigue. Our findings provide a comprehensive understanding of the development of muscle fatigue, highlighting the interconnectedness of mechanical, electrical, and metabolic responses during submaximal efforts. The reduction in force-generation capacity due to muscle fatigue is reflected in the S EMG signal and manifests as an increase in motor variability. This study identified changes in the EMG and NIRS parameters, and significant interrelation between the two metrics during the process of fatigue accumulation. These findings have the potential to provide crucial knowledge for the prediction of fatigue from either EMG signal or hemodynamic signals of the muscles. ⓘ Song, Junkyung; Choi, Yoon-Seok; Lee, Sungjune; Park, Dawon; Park, Jaebum ⓘ Seoul Natl Univ, Dept Phys Educ, Seoul, South Korea; Kyungpook Natl Univ, Dept Kinesiol, Sangju, South Korea; Seoul Natl Univ, Adv Inst Convergence Technol, Seoul, South Korea; Seoul Natl Univ, Dept AI Integrated Educ, Seoul, South Korea; Seoul Natl Univ, Inst Sports Sci, Seoul, South Korea ⓘ ⓘ 57218670094; 59736930700; 58027376100; 57470124900; 8309988900 ⓘ parkpe95@snu.ac.kr; ⓘ FRONTIERS IN PHYSIOLOGY ⓘ FRONT PHYSIOL ⓘ 1664-042X 16 SCIE PHYSIOLOGY ⓘ 2024 3.4 22.4 0 2025-05-07 ⓘ 0 1 surface electromyography; near-infrared spectroscopy; median-power-frequency; tissue oxygenation index; vastus lateralis muscle hemodynamics and EMG during repetitive-contraction ⓘ SURFACE EMG; KNEE EXTENSION; ELECTROMYOGRAPHY; MECHANOMYOGRAPHY; COORDINATION; VARIABILITY; CONSUMPTION; AGE ⓘ median-power-frequency; near-infrared spectroscopy; surface electromyography; tissue oxygenation index; vastus lateralis muscle hemodynamics and EMG during repetitive-contraction ⓘ adult; Article; controlled study; correlation analysis; electric potential; electromyogram; electromyography; fatigue; hemodynamics; human; knee; knee extension angle; knowledge; male; median power frequency; muscle contraction; muscle fatigue; muscle isometric contraction; near infrared spectroscopy; neuropathy; normal human; oxygen saturation; oxygen supply; oxygenation; skinfold thickness; surface electromyography; task positive network; tissue oxygen saturation index; tissue oxygenation; torque; vastus lateralis muscle ⓘ English 2025 2025-04-02 10.3389/fphys.2025.1559893 ⓘ 바로가기 바로가기 바로가기 바로가기
○ ○ Article ⓘ Chronic Low-Level IFN-γ Expression Disrupts Mitochondrial Complex I Activity in Renal Macrophages: An Early Mechanistic Driver of Lupus Nephritis Pathogenesis ⓘ Mitochondrial dysfunction and macrophage dysregulation are well recognized as significant contributors to the pathogenesis of autoimmune diseases. However, the detailed mechanisms connecting these two factors remain poorly understood. This study hypothesizes that low but chronic interferon-gamma (IFN-gamma) plays a critical role in these processes. To explore this, we utilized ARE-Del mice, a model characterized by sustained low-level IFN-gamma expression and lupus nephritis (LN)-like symptoms. Age- and tissue-dependent gene expression analyses in ARE-Del mice revealed significant suppression of mitochondrial complex I components and activities, particularly in the kidneys. The genotype-dependent suppression of mitochondrial complex I indicates early disruption, which leads to macrophage dysfunction. Notably, remission restored gene expression of mitochondrial complex I and macrophage dysfunction in isolated renal macrophages from NZB/W lupus-prone mice. These findings suggest that chronic low-level IFN-gamma disrupts mitochondrial complex I activity in macrophages, highlighting its role in the early pathogenesis of autoimmune diseases like lupus nephritis. This provides new insights into the molecular interactions underlying autoimmune pathogenesis and suggests potential targets for therapeutic intervention. ⓘ Bae, Heekyong R.; Shin, Su-Kyung; Lee, Ji-Yoon; Ko, Yeo Jin; Kim, Suntae; Young, Howard A.; Kwon, Eun-Young ⓘ Kyungpook Natl Univ, Dept Food Sci & Nutr, Daegu 41566, South Korea; Kyungpook Natl Univ, Ctr Food & Nutr Genom, Daegu 41566, South Korea; Omixplus LLC, Gaithersburg, MD 20850 USA; NCI, Canc Innovat Lab, Ctr Canc Res, Frederick, MD 21702 USA; Kyungpook Natl Univ, Ctr Beautiful Aging, Daegu 41566, South Korea ⓘ ; Young, Howard/R-5140-2019 ⓘ 57191253762; 23988149900; 58631087000; 59506529600; 57221230845; 57202981972; 15765422500 ⓘ baehee@knu.ac.kr; eykwon@knu.ac.kr; ⓘ INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES ⓘ INT J MOL SCI ⓘ 1661-6596 1422-0067 26 1 SCIE BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY ⓘ 2024 4.9 22.4 0 2025-05-07 ⓘ 0 0 interferon gamma; mitochondrial complex I; macrophage dysfunction; lupus nephritis; autoimmune diseases ⓘ INTERFERON; TRANSLATION; METABOLISM; ACTIVATION; AUTOPHAGY ⓘ autoimmune diseases; interferon gamma; lupus nephritis; macrophage dysfunction; mitochondrial complex I ⓘ Animals; Disease Models, Animal; Electron Transport Complex I; Female; Interferon-gamma; Kidney; Lupus Nephritis; Macrophages; Mice; Mice, Inbred C57BL; Mitochondria; gamma interferon; reduced nicotinamide adenine dinucleotide dehydrogenase (ubiquinone); gamma interferon; reduced nicotinamide adenine dinucleotide dehydrogenase (ubiquinone); age; animal cell; animal experiment; animal model; animal tissue; Article; blood sampling; cell isolation; controlled study; enzyme activity; gene expression profiling; genotype; human; human tissue; kidney cell; kidney tissue; lupus erythematosus nephritis; macrophage; mouse; nonhuman; pathogenesis; protein expression level; remission; spleen tissue; thymus tissue; animal; C57BL mouse; disease model; female; genetics; kidney; lupus erythematosus nephritis; metabolism; mitochondrion; pathology ⓘ English 2025 2025-01 10.3390/ijms26010063 ⓘ 바로가기 바로가기 바로가기 바로가기
○ ○ Review ⓘ Coffee and Its Major Polyphenols in the Prevention and Management of Type 2 Diabetes: A Comprehensive Review ⓘ Type 2 diabetes mellitus (T2DM) is a chronic metabolic disorder characterized by insulin resistance and impaired glucose metabolism and affects a substantial portion of the global population. Over the past few decades, numerous studies have investigated lifestyle factors, including diet and physical activity, as preventive measures or adjunctive treatments for T2DM. Among the dietary factors, coffee consumption has garnered attention because of its potential to mitigate the risk and progression of T2DM. This review examines the current evidence on the relationship between coffee consumption and T2DM, with particular focus on the major polyphenols found in coffee, such as chlorogenic acid and related hydroxycinnamic acids (caffeic acid, ferulic acid, p-coumaric acid, and sinapic acid). These bioactive compounds are thought to exert anti-diabetic effects through several mechanisms, including improvements in glucose homeostasis, insulin sensitivity, inflammation, and oxidative stress. This review aimed to clarify the scientific rationale behind the potential therapeutic effects of coffee on T2DM and proposed directions for future studies. However, significant knowledge gaps remain, including limited clinical evidence, unclear optimal dosages, low bioavailability, and an incomplete understanding of molecular mechanisms. Addressing these gaps through well-designed clinical trials and advanced molecular studies is essential to fully establish the therapeutic potential of coffee and its polyphenols in T2DM. ⓘ Kim, Hwicheol; Kim, Sang Ryong; Jung, Un Ju ⓘ Pukyong Natl Univ, Dept Food Sci & Nutr, 45 Yongso Ro, Busan 48513, South Korea; Kyungpook Natl Univ, Sch Life Sci & Biotechnol, BK21 FOUR KNU Creat Biores Grp, 80 Daehak Ro, Daegu 41566, South Korea ⓘ ⓘ 59551696100; 59961590400; 59653597700 ⓘ k423897@naver.com; srk75@knu.ac.kr; jungunju@pknu.ac.kr; ⓘ INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES ⓘ INT J MOL SCI ⓘ 1661-6596 1422-0067 26 12 SCIE BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY ⓘ 2024 4.9 22.4 ⓘ 0 0 type 2 diabetes mellitus; coffee; polyphenols; chlorogenic acid; caffeic acid; ferulic acid; p-coumaric acid; sinapic acid ⓘ P-COUMARIC ACID; HIGH-FAT DIET; FERULIC ACID; CHLOROGENIC ACID; CAFFEIC ACID; PHENOLIC-ACIDS; DECAFFEINATED COFFEE; GLUCOSE-METABOLISM; INSULIN-SECRETION; SINAPIC ACID ⓘ caffeic acid; chlorogenic acid; coffee; ferulic acid; p-coumaric acid; polyphenols; sinapic acid; type 2 diabetes mellitus ⓘ caffeic acid; chlorogenic acid; coumaric acid; ferulic acid; polyphenol; sinapic acid; antidiabetic activity; bioavailability; coffee; coffee consumption; glucose homeostasis; glucose metabolism; human; insulin resistance; insulin sensitivity; metabolic disorder; non insulin dependent diabetes mellitus; oxidative stress; pharmacokinetics; physical activity; prevention; review; therapy ⓘ English 2025 2025-06-10 10.3390/ijms26125544 ⓘ 바로가기 바로가기 바로가기 바로가기
○ ○ Article ⓘ Enhancing Soybean Salt Tolerance with GSNO and Silicon: A Comprehensive Physiological, Biochemical, and Genetic Study ⓘ Soil salinity is a major global challenge affecting agricultural productivity and food security. This study explores innovative strategies to improve salt tolerance in soybean (Glycine max), a crucial crop in the global food supply. This study investigates the synergistic effects of S-nitroso glutathione (GSNO) and silicon on enhancing salt tolerance in soybean (Glycine max). Two soybean cultivars, Seonpung (salt-tolerant) and Cheongja (salt-sensitive), were analyzed for various physiological, biochemical, and genetic traits under salt stress. The results showed that the combined GSNO and Si treatment significantly improved several key traits, including plant height, relative water content, root development, nodule numbers, chlorophyll content, and stomatal aperture, under both control and salt stress conditions. Additionally, this treatment optimized ion homeostasis by enhancing the Na/K ratio and Ca content, while reducing damage markers such as electrolyte leakage, malondialdehyde, and hydrogen peroxide. The stress-responsive compounds, including proline, ascorbate peroxidase, and water-soluble proteins, were elevated under stress conditions, indicating improved tolerance. Gene expression analysis revealed significant upregulation of genes such as GmNHX1, GmSOS2, and GmAKT1, associated with salt stress response, while GmNIP2.1, GmNIP2.2, and GmLBR were downregulated in both varieties. Notably, the salt-sensitive variety Cheongja exhibited higher electrolyte leakage and oxidative damage compared to the salt-tolerant Seonpung. These findings suggest that the combination of GSNO and silicon enhances salt tolerance in soybean by improving physiological resilience, ion homeostasis, and stress-responsive gene expression. ⓘ Msarie, Meshari Winledy; Methela, Nusrat Jahan; Islam, Mohammad Shafiqul; An, Tran Hoang; Das, Ashim Kumar; Lee, Da-Sol; Mun, Bong-Gyu; Yun, Byung-Wook ⓘ Kyungpook Natl Univ, Coll Agr & Life Sci, Dept Food Secur & Agr Dev, Daegu 41566, South Korea; Kyungpook Natl Univ, Coll Agr & Life Sci, Dept Appl Biosci, Daegu 41566, South Korea; Noakhali Sci & Technol Univ, Dept Agr, Noakhali 3814, Bangladesh; Chungbuk Natl Univ, Dept Environm & Biol Chem, Cheongju 28644, South Korea ⓘ Das, Ashim Kumar/ABB-6751-2020; Mun, BongGyu/GYD-6010-2022 ⓘ 59527233700; 57880065700; 59448717500; 59527888800; 57211473983; 57222624235; 57147241300; 8245123600 ⓘ methela.ag@nstu.edu.bd; shafik.ag@nstu.edu.bd; munbg@cbnu.ac.kr; bwyun@knu.ac.kr; ⓘ INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES ⓘ INT J MOL SCI ⓘ 1661-6596 1422-0067 26 2 SCIE BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY ⓘ 2024 4.9 22.4 0 2025-05-07 ⓘ 1 1 salt tolerance; ion homeostasis; ROS; gene expression ⓘ NITRIC-OXIDE; SALINITY TOLERANCE; PLANT-RESPONSES; STRESS; GROWTH; ACID; CHLOROPLASTS; METABOLISM; DROUGHT ⓘ gene expression; ion homeostasis; ROS; salt tolerance ⓘ Gene Expression Regulation, Plant; Glutathione; Glycine max; Plant Proteins; Plant Roots; Salt Stress; Salt Tolerance; Silicon; ascorbate peroxidase; calcium ion; chlorophyll a; chlorophyll b; electrolyte; glutathione; hydrogen peroxide; leghemoglobin; malonaldehyde; nip2.1 protein; nip2.2 protein; nodulin 26 like in protein; potassium; potassium channel; proline; reactive oxygen metabolite; s nitroso glutathione; salt overlay sensitive pathway gene protein; silicon; sodium; sodium chloride; sodium proton exchange protein; unclassified drug; glutathione; plant protein; silicon; Article; biochemical analysis; chlorophyll content; controlled study; cultivar; down regulation; gene expression; gene expression profiling; genetic analysis; genetic trait; homeostasis; nonhuman; physiological process; plant growth; plant height; root development; root length; root morphology; salt sensitivity; salt stress; salt tolerance; soil salinity; soil salinization; soybean; synergistic effect; water content; drug effect; gene expression regulation; genetics; growth, development and aging; metabolism; plant root ⓘ English 2025 2025-01 10.3390/ijms26020609 ⓘ 바로가기 바로가기 바로가기 바로가기
○ ○ Article ⓘ Identification of Phytosphingosine-Based 1-O-Acylceramide in Human Stratum Corneum and Investigation of Its Role in Skin Barrier ⓘ Phytosphingosine-based 1-O-acylceramide (CerENP) from the human stratum corneum has not been reported on. High-resolution mass spectrometry was used to identify CerENP from skin samples. A vehicle-controlled human study was performed to investigate the physiological interaction between ceramide NP (CerNP) and CerENP with respect to skin hydration, cohesion, and TEWL, all of which were measured. Twenty volunteers were treated with test creams containing CerENP together with CerNP, which significantly improved skin barrier parameters after four weeks of application: 1. Skin hydration was increased by 26% compared to when CerNP was used alone, and moisture retention was better than it was with the CerNP control. 2. Stratum corneum (SC) cohesion was strengthened significantly only when using the test cream formulated with CerENP. The results suggested the boosting effects of CerENP on the skin barrier functions exerted by CerNP since only a small amount is required, as low as one-tenth of CerNP. This is the first report on the identification of CerENP in the human SC and its skin barrier activities in human skin. In conclusion, the combinatorial use of CerENP and CerNP at an appropriate relative ratio could be a new normal in developing an ideal moisturizer for dry and atopic skin. ⓘ Kang, Bae-Gon; Choi, Hyun Kyung; Liu, Kwang-Hyeon; Hong, Sung Kyu; Kim, Jin Wook; Lee, Eun Ok; Park, Chang Seo ⓘ Kyungpook Natl Univ, Coll Pharm, Based Intelligent Novel Drug Discovery Educ Unit, BK21 FOUR Community, Daegu 41566, South Korea; Dongguk Univ, Dept Chem & Biochem Engn, Seoul 04620, South Korea; LCS Biotech, Yongin 17130, South Korea ⓘ Kim, Jin/GSD-4756-2022 ⓘ 58068275800; 35793240100; 55768214700; 55726356400; 59751080300; 57203206504; 56003129200 ⓘ baegon0709@naver.com; bluentia@naver.com; dstlkh@knu.ac.kr; hsk5457@dongguk.edu; deacne@lcsbio.com; leeeunok@lcsbio.com; csp54@lcsbio.com; ⓘ COSMETICS ⓘ COSMETICS-BASEL ⓘ 2079-9284 12 2 ESCI BIOCHEMISTRY & MOLECULAR BIOLOGY;DERMATOLOGY ⓘ 2024 3.2 22.4 0 2025-05-07 ⓘ 0 0 1-O-acylceramide; CerENP; identification; skin barrier; boosting effect ⓘ DIVERSE CHAIN LENGTHS; ATOPIC-DERMATITIS; FATTY-ACIDS; LIPID-MEMBRANES; CERAMIDES; MICROSTRUCTURE; PERMEABILITY; ORGANIZATION; HEALTHY; IMPROVE ⓘ 1-O-acylceramide; boosting effect; CerENP; identification; skin barrier ⓘ ⓘ English 2025 2025-03-11 10.3390/cosmetics12020047 ⓘ 바로가기 바로가기 바로가기 바로가기
○ ○ Article ⓘ Inhibition of MCP1 (CCL2) Enhances Antitumor Activity of NK Cells Against HCC Cells Under Hypoxia ⓘ Hypoxia, a low-oxygen state, is a common feature of solid tumors. MCP1 (CCL2) is a small cytokine that is closely related to hypoxia and has a positive effect on tumor development. Hypoxia causes resistance to various treatments for solid tumors and the evasion of cancer immune surveillance by lymphocytes. Natural killer (NK) cells are innate lymphocytes that play an important role in cancer development, particularly in the liver. First, it was found that the incubation of HCC in hypoxia (2-5% O2) significantly increased the production of several inflammatory cytokines, including MCP1, compared to that of normal oxygen (20% O2). Subsequently, blocking MCP1 with an anti-MCP1 antibody in HCC cultures inhibited the growth and migration of HCC cells in vitro and in vivo. This was associated with a decrease in the expression of HIF-1 alpha/STAT3 in HCC under hypoxia. Furthermore, blocking MCP1 in HCC cell cultures under hypoxia significantly increased the chemotaxis and activation of NK-92 cells against HCC cells. MCP1 blockade in HCC cell cultures under hypoxia induced a shift in NK cells to the CD56+dim population and an increase in the expression of the activation receptors NKG2D and NKp44. In conclusion, modulation of MCP1 could enhance NK activity against hypoxic HCC cells. ⓘ Lee, Hwan Hee; Kim, Juhui; Park, Eunbi; Kang, Hyojeung; Cho, Hyosun ⓘ Duksung Womens Univ, Coll Pharm, Seoul 01369, South Korea; Duksung Womens Univ, Duksung Innovat Drug Ctr, Seoul 01369, South Korea; Kyungpook Natl Univ, Canc Res Inst, Coll Pharm, Vessel Organ Interact Res Ctr,VOICE MRC, Daegu 41566, South Korea ⓘ ⓘ 59917008600; 59917719100; 59917478800; 8979751700; 55572361200 ⓘ oeo3oeo@gmail.com; kimjuhui@duksung.ac.kr; peb8956@duksung.ac.kr; hkang72@knu.ac.kr; hyosun1102@duksung.ac.kr; ⓘ INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES ⓘ INT J MOL SCI ⓘ 1661-6596 1422-0067 26 10 SCIE BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY ⓘ 2024 4.9 22.4 0 2025-06-11 ⓘ 0 0 MCP1 (CCL2); hypoxia; natural killer (NK) cell; tumor microenvironment; human hepatocellular carcinoma (HCC) ⓘ CANCER PROGRESSION; TUMOR PROGRESSION; INDUCIBLE FACTORS; AXIS; PROMOTES; MICROENVIRONMENT; MECHANISMS; EXPRESSION; PROTEIN-1; PROGNOSIS ⓘ human hepatocellular carcinoma (HCC); hypoxia; MCP1 (CCL2); natural killer (NK) cell; tumor microenvironment ⓘ Animals; Carcinoma, Hepatocellular; Cell Hypoxia; Cell Line, Tumor; Cell Movement; Chemokine CCL2; Humans; Hypoxia-Inducible Factor 1, alpha Subunit; Killer Cells, Natural; Liver Neoplasms; Mice; STAT3 Transcription Factor; CD56 antigen; gelatinase B; hypoxia inducible factor 1alpha; interleukin 6; monocyte chemotactic protein 1; monocyte chemotactic protein 1 antibody; monocyte chemotactic protein 1 inhibitor; nerve cell adhesion molecule; oxygen; STAT3 protein; unclassified drug; CCL2 protein, human; hypoxia inducible factor 1alpha; monocyte chemotactic protein 1; STAT3 protein; animal cell; animal experiment; animal model; animal tissue; antineoplastic activity; antitumorigenic activity; apoptosis; Article; cancer cell culture; cancer inhibition; carcinoma cell; cell population; chemotaxis; controlled study; cytokine production; female; human; human cell; in vitro study; in vivo study; liver cell carcinoma; metastasis; metastasis inhibition; migration inhibition; mouse; natural killer cell mediated cytotoxicity; NK-92 cell line; nonhuman; protein expression; tumor hypoxia; animal; cell hypoxia; cell motion; immunology; liver cell carcinoma; liver tumor; metabolism; natural killer cell; pathology; tumor cell line ⓘ English 2025 2025-05-20 10.3390/ijms26104900 ⓘ 바로가기 바로가기 바로가기 바로가기
○ ○ Article ⓘ Inhibitory Effects of Heat-Processed Gynostemma pentaphyllum Extract (Actiponin®) and Its Components on Cartilage Breakdown in Osteoarthritis ⓘ Osteoarthritis (OA), caused by the long-term use of joints, is a representative degenerative disease in the elderly. However, recently, the age of onset has been decreasing owing to excessive activities among young people in their 20s and 30s. Gynostemma pentaphyllum (Thunb.) Makino (GP), a perennial herb of the Cucurbitaceae family, has been used since the Ming dynasty as a medicinal material to treat various ailments, such as rheumatism, liver disease, and diabetes. In this study, we investigated the anti-arthritic effects of heat-processed Gynostemma pentaphyllum extract (Actiponin (AP)) and its derivatives, damulin A (DA) and damulin B (DB), using in vitro (primary rat chondrocytes and SW1353 cells) and in vivo (destabilization of the medial meniscus (DMM)-induced OA model) systems. Histological analysis results from the in vivo study showed that the group that underwent DMM surgery induced degeneration by the loss of proteoglycan and the destruction of cartilage (OARSI score 14 +/- 0.57), whereas the group that received AP daily for 8 weeks maintained an intact condition (OARSI score 5 +/- 0.28 at 200 mg/kg, p < 0.001). In addition, cartilage thickness and chondrocytes were reduced in the DMM group, but were restored in the AP-administered group. Furthermore, the von Frey analysis results showed that the pain threshold of the DMM group was considerably low (54.5 g at 8 weeks), whereas that of the AP group was dose-dependently increased (65.5, 69.5, 70.3, and 71.8 at 8 weeks for 30, 50, 100, and 200 mg/kg, respectively). In vitro studies showed that AP, DA, and DB reduced the expression of interleukin-1 beta alone-induced nitrite; inducible nitric oxide synthase; cyclooxygenase-2; matrix metallopeptidase 1/3/13; and a disintegrin and metalloproteinase with thrombospondin motifs 4/5. They also restored the expression of collagen type II and aggrecan, which are components of the extracellular matrix. The anti-arthritic effects of AP, DA, and DB were confirmed to be mediated by the mitogen-activated protein kinase and nuclear factor kappa-light-chain-enhancer of activated B cell signaling pathways. Collectively, these results suggest that AP is a potential therapeutic agent for mitigating OA progression and chondroprotection. ⓘ Lee, Seul Ah; Lee, Chan Hwi; Lee, Sun Hee; Do, Eunju; Kim, Do Kyung; Huh, Tae-Lin; Kim, Chun Sung ⓘ Chosun Univ, Coll Dent, Dept Oral Biochem, Gwangju 61452, South Korea; Kyungpook Natl Univ, TG Biotech Res Inst, Technobuilding, 47,Gyeongdae Ro 17-gil, Daegu 41566, South Korea; Chosun Univ, Coll Dent, Dept Oral Biol, Gwangju 61452, South Korea ⓘ ⓘ 57225122738; 59660621200; 58607352900; 59307854300; 57219213298; 7007119367; 35272317800 ⓘ seulah21@naver.com; cksgnl0201@tgbio.com; ihappy278@tgbio.com; ejdo2302@tgbio.com; kdk@chosun.ac.kr; tlhuh56@tgbio.com; cskim2@chosun.ac.kr; ⓘ INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES ⓘ INT J MOL SCI ⓘ 1661-6596 1422-0067 26 4 SCIE BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY ⓘ 2024 4.9 22.4 0 2025-05-07 ⓘ 0 0 Gynostemma pentaphyllum; actiponin; osteoarthritis; chondrocytes; anti-arthritic ⓘ PROGRESSION; CROSSTALK; MAPK; MICE ⓘ actiponin; anti-arthritic; chondrocytes; Gynostemma pentaphyllum; osteoarthritis ⓘ Animals; Cartilage, Articular; Chondrocytes; Disease Models, Animal; Gynostemma; Hot Temperature; Humans; Male; Osteoarthritis; Plant Extracts; Rats; Rats, Sprague-Dawley; actiponin; aggrecan; aggrecanase 1; aggrecanase 2; collagen type 2; cyclooxygenase 2; Gynostemma pentaphyllum extract; gypenoside; inducible nitric oxide synthase; interleukin 1beta; matrix metalloproteinase; mitogen activated protein kinase; nitrite; proteoglycan; plant extract; aged; animal cell; animal experiment; animal model; Article; B lymphocyte; cartilage; cartilage thickness; chondrocyte; chondroprotection; controlled study; drug effect; extracellular matrix; female; Gynostemma pentaphyllum; heat treatment; histology; in vitro study; in vivo study; male; medial meniscus; nonhuman; onset age; osteoarthritis; pain threshold; protein degradation; protein expression; rat; signal transduction; SW1353 cell line; animal; articular cartilage; chemistry; chondrocyte; disease model; drug effect; drug therapy; Gynostemma; high temperature; human; metabolism; osteoarthritis; pathology; Sprague Dawley rat ⓘ English 2025 2025-02 10.3390/ijms26041728 ⓘ 바로가기 바로가기 바로가기 바로가기
○ ○ Article ⓘ Integration of Genetic and Imaging Data to Detect QTL for Root Traits in Interspecific Soybean Populations ⓘ Wild soybean, which has many desirable traits, such as adaptability to climate change-related stresses, is a valuable resource for expanding the narrow genetic diversity of cultivated soybeans. Plants require roots to adapt to different environments and optimize water and nutrient uptake to support growth and facilitate the storage of metabolites; however, it is challenging and costly to evaluate root traits under field conditions. Previous studies of quantitative trait loci (QTL) have been mainly based on cultivated soybean populations. In this study, an interspecific mapping population from a cross between wild soybean 'PI483463' and cultivar 'Hutcheson' was used to investigate QTLs associated with root traits using image data. Our results showed that 39 putative QTLs were distributed across 10 chromosomes (chr.). Seventeen of these were clustered in regions on chr. 8, 14, 15, 16, and 17, accounting for 19.92% of the phenotypic variation. We identified five significant QTL clusters influencing root-related traits, such as total root length, surface area, lateral total length, and number of tips, across five chr., with favorable alleles from both wild and cultivated soybeans. Furthermore, we identified eight candidate genes controlling these traits based on functional annotation. These genes were highly expressed in root tissues and directly or indirectly affected soybean root growth, development, and stress responses. Our results provide valuable insights for breeders aiming to optimize soybean root traits and leveraging genetic diversity from wild soybean species to develop varieties with improved root morphological traits, ultimately enhancing overall plant growth, productivity, and resilience. ⓘ Islam, Mohammad Shafiqul; Lee, Jeong-Dong; Song, Qijian; Jo, Hyun; Kim, Yoonha ⓘ Kyungpook Natl Univ, Dept Appl Biosci, Daegu 41566, South Korea; Kyungpook Natl Univ, Dept Integrat Biol, Daegu 41566, South Korea; Noakhali Sci & Technol Univ, Dept Agr, Noakhali 3814, Bangladesh; Kyungpook Natl Univ, Upland Field Machinery Res Ctr, Daegu 41566, South Korea; USDA ARS, Soybean Genom & Improvement Lab, Beltsville Agr Res Ctr, Beltsville, MD 20705 USA ⓘ ⓘ 59448717500; 40462075100; 7202973814; 57194539358; 57224866763 ⓘ shafik.hort@gmail.com; jdlee@knu.ac.kr; qijian.song@usda.gov; johyun@knu.ac.kr; kyh1229@knu.ac.kr; ⓘ INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES ⓘ INT J MOL SCI ⓘ 1661-6596 1422-0067 26 3 SCIE BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY ⓘ 2024 4.9 22.4 0 2025-05-07 ⓘ 1 1 soybean; root morphological traits; RILs; QTL; SNP; candidate genes ⓘ RECEPTOR-LIKE KINASES; ORYZA-SATIVA L.; BINDING-PROTEIN; P-GLYCOPROTEIN; STRESS; GROWTH; IDENTIFICATION; EXPRESSION; TOLERANCE; TRANSPORTERS ⓘ candidate genes; QTL; RILs; root morphological traits; SNP; soybean ⓘ Chromosome Mapping; Chromosomes, Plant; Glycine max; Phenotype; Plant Roots; Quantitative Trait Loci; allele; Article; cultivar; gene expression; gene identification; genetic analysis; genetic variability; genetic variation; metabolite; morphological trait; nonhuman; nutrient uptake; phenotypic variation; plant growth; plant root; quantitative trait locus; root growth; root length; single nucleotide polymorphism; soybean; X chromosome; chromosomal mapping; genetics; growth, development and aging; phenotype; plant chromosome ⓘ English 2025 2025-02 10.3390/ijms26031152 ⓘ 바로가기 바로가기 바로가기 바로가기
○ ○ Review ⓘ Liver Metabolism at the Crossroads: The Reciprocal Control of Nutrient-Sensing Nuclear Receptors and Autophagy ⓘ Peroxisome proliferator-activated receptor alpha (PPAR alpha, encoded by NR1C1) and farnesoid X receptor (FXR, encoded by NR1H4) are the two prominent nutrient-sensing nuclear receptors essential for maintaining hepatic metabolism during fasting and fed states, respectively. These nuclear receptors comprehensively regulate the transcription of numerous genes involved in fatty acid oxidation (FAO), ketogenesis, bile acid (BA) biosynthesis, and other metabolic processes critical for liver energy homeostasis. These receptors have been shown to have opposite impacts on autophagy, which is triggered by PPAR alpha activation but inhibited by FXR activation. Recent studies have further revealed that liver-specific genetic ablation of key autophagic genes tremendously impairs the activation of these nuclear receptors, thereby profoundly affecting hepatic metabolism in both fasting and feeding states. This review explores the roles and mechanisms of PPAR alpha and FXR in regulating liver metabolism and autophagy, highlighting the necessity of basal autophagic activity in ensuring the proper signaling of these nutrient-sensing nuclear receptors. Finally, we examine the potential therapeutic strategies that leverage the interplay between PPAR alpha, FXR, and autophagy for the treatment of metabolic liver disorders. We also delve into the clinical implications of this complex relationship, emphasizing its significance for translational medicine and future therapeutic interventions. ⓘ Kim, Eun Young; Lee, Jae Man ⓘ Kyungpook Natl Univ, Cell & Matrix Res Inst, Sch Med, Dept Biochem & Cell Biol, 680 Gukchaebosang Ro, Daegu 41944, South Korea; Kyungpook Natl Univ, Grad Sch, Dept Biomed Sci, BK21 KNU Biomed Convergence Program Biomed Sci Cre, Daegu 41944, South Korea ⓘ Lee, Jae/KFF-5361-2024; Kim, YoungHwan/B-2395-2013 ⓘ 57194632567; 47461355200 ⓘ key11@knu.ac.kr; jaemanlee@knu.ac.kr; ⓘ INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES ⓘ INT J MOL SCI ⓘ 1661-6596 1422-0067 26 12 SCIE BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY ⓘ 2024 4.9 22.4 ⓘ 0 0 nuclear receptor; PPAR alpha; FXR; autophagy; liver ⓘ PROLIFERATOR-ACTIVATED-RECEPTOR; FARNESOID-X-RECEPTOR; ALPHA PPAR-ALPHA; FATTY-ACID OXIDATION; BILE-ACID; GENE-EXPRESSION; PROTEIN-KINASE; LIPID-METABOLISM; GROWTH-HORMONE; DEFICIENT MICE ⓘ autophagy; FXR; liver; nuclear receptor; PPARα ⓘ bile acid; cell nucleus receptor; farnesoid X receptor; peroxisome proliferator activated receptor alpha; autophagic cell death; autophagy (cellular); biosynthesis; drug therapy; fasting; fatty acid oxidation; gene expression; homeostasis; human; ketogenesis; liver; liver metabolism; pharmacology; review; signal transduction ⓘ English 2025 2025-06-18 10.3390/ijms26125825 ⓘ 바로가기 바로가기 바로가기 바로가기
○ ○ Article ⓘ Longitudinal analysis of coal workers' pneumoconiosis using enhanced resolution-computed tomography images: unveiling patterns in lung structure, function, and clinical correlations ⓘ Rationale Pneumoconiosis, caused by prolonged exposure to mineral dust, leads to progressive structural and functional lung alterations. Quantitative computed tomography (qCT) has emerged as a critical tool for assessing these changes, yet there is limited research on the longitudinal patterns in pneumoconiosis patients.Methods This study examined a cohort of 31 former coal workers with pneumoconiosis over a 1-year period. Inspiratory qCT images were enhanced using a deep learning-based super-resolution model and then processed to extract lung functional and airway structural metrics. A non-rigid image registration process was performed with baseline images as fixed and follow-up images as moving. Registration-derived metrics, including anisotropic deformation index (ADI), slab rod index (SRI), and Jacobian (J), were extracted to quantify regional deformation longitudinally. Pulmonary function tests, including forced expiratory volume in one second (FEV1) and forced vital capacity (FVC), were recorded at both time points to assess functional decline.Results The study identified significant airway changes in angles, diameters, and geometry, with a decrease in normal lung tissue in the right upper lobe. Blood vessel volumes declined, indicating vascular remodeling. Registration metrics revealed regional heterogeneity, with higher ADI and SRI values and localized volume loss (J) in the lower lobes. FEV1/FVC progression correlated positively with tracheal angle, emphysema, and consolidation but negatively with normal lung tissue, semi-consolidation, and fibrosis. ADI, SRI, and J were associated with structural deformation, airway remodeling, and parenchymal loss, linking these changes to lung function decline.Conclusion qCT imaging and registration metrics effectively monitor structural and functional lung changes in pneumoconiosis. Registering baseline and follow-up inspiration images offers additionally valuable insights into disease progression. ⓘ Chau, Ngan-Khanh; Park, Eun-Kee; Choi, Sanghun ⓘ Kyungpook Natl Univ, Sch Mech Engn & IEDT, Daegu, South Korea; Vietnam Natl Univ Ho Chi Minh City, An Giang Univ, Ho Chi Minh, Vietnam; Kosin Univ, Coll Med, Dept Prevent Med, Busan, South Korea ⓘ Ngân Khánh, Châu/IUM-5515-2023 ⓘ 57658928300; 57216814178; 55847101000 ⓘ s-choi@knu.ac.kr; eunkee.park@gmail.com; ⓘ FRONTIERS IN PHYSIOLOGY ⓘ FRONT PHYSIOL ⓘ 1664-042X 16 SCIE PHYSIOLOGY ⓘ 2024 3.4 22.4 N/A ⓘ 0 0 coal workers with pneumoconiosis; longitudinal analysis; lung structure; lung function; clinical implications; disease progression ⓘ EMPHYSEMA; OBSTRUCTION; PNEUMONIA; DISEASE ⓘ clinical implications; coal workers with pneumoconiosis; disease progression; longitudinal analysis; lung function; lung structure ⓘ aged; Article; coal worker; cohort analysis; computer assisted tomography; controlled study; deep learning; female; follow up; forced expiratory volume; forced vital capacity; geometry; human; image quality; long term exposure; longitudinal study; lung development; lung function; lung function test; lung structure; male; mineral dust; pneumoconiosis; total lung capacity ⓘ English 2025 2025-05-30 10.3389/fphys.2025.1578058 ⓘ 바로가기 바로가기 바로가기 바로가기
○ ○ Article ⓘ Molecular Structure of the mRNA Export Factor Gle1 from Debaryomyces hansenii ⓘ Gle1 functions as a regulator of Dbp5, a DEAD-box-containing RNA helicase that is a component of the nuclear pore complex. In association with Gle1 and inositol hexakisphosphate (IP6), ADP-bound Dbp5 facilitates the release of RNA. The RNA-bound Dbp5 undergoes ATP hydrolysis and is activated by Gle1 in the presence of IP6. The formation of a ternary complex involving Dbp5, Gle1, and the nucleoporin Nup159 promotes ADP secretion and prevents RNA recombination. To date, several complex structures of Gle1 with its binding partners have been described; however, the structure of unbound Gle1 remains elusive. To investigate the structural features associated with complex formation, the crystal structure of N-terminally truncated Gle1 from Debaryomyces hansenii (DhGle1 Delta N) was determined at a resolution of 1.5 & Aring;. The DhGle1 Delta N protein comprises 13 alpha-helices. Structural comparisons with homologs, all of which have been characterized in various complexes, revealed no significant conformational changes. However, several distinct secondary structural elements were identified in alpha 1, alpha 3, alpha 4, and alpha 8. This study may provide valuable insights into the architecture of yeast Gle1 proteins and their interactions with Dbp5, which is crucial for understanding the regulation of mRNA export. ⓘ Jang, Min Jeong; Lee, Soo Jin; Chang, Jeong Ho ⓘ Kyungpook Natl Univ, Dept Biol Educ, 80 Daehak Ro, Daegu 41566, South Korea; Kyungpook Natl Univ, Dept Biomed Convergence Sci & Technol, 80 Daehak Ro, Daegu 41566, South Korea; Kyungpook Natl Univ, Sci Educ Res Inst, 80 Daehak Ro, Daegu 41566, South Korea ⓘ ⓘ 59655031400; 59654765500; 57203598905 ⓘ jhcbio@knu.ac.kr; ⓘ INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES ⓘ INT J MOL SCI ⓘ 1661-6596 1422-0067 26 4 SCIE BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY ⓘ 2024 4.9 22.4 0 2025-05-07 ⓘ 0 0 mRNA export; nuclear pore complex; Dbp5; Gle1; IP6; Debaryomyces hansenii ⓘ NUCLEAR-PORE COMPLEX; BOX PROTEIN DBP5; DEAD-BOX; HELICASE; NUP159; ACTIVATION; INTERACTS; INSP(6); CYCLE; KEY ⓘ Dbp5; Debaryomyces hansenii; Gle1; IP6; mRNA export; nuclear pore complex ⓘ Amino Acid Sequence; Crystallography, X-Ray; DEAD-box RNA Helicases; Debaryomyces; Fungal Proteins; Models, Molecular; Nucleocytoplasmic Transport Proteins; Protein Binding; RNA, Messenger; Saccharomycetales; messenger RNA; DEAD box protein; fungal protein; messenger RNA; nucleocytoplasmic transport protein; protein binding; alpha helix; Article; chemical structure; complex formation; conformational transition; controlled study; Debaryomyces hansenii; nonhuman; amino acid sequence; budding yeast; chemistry; Debaryomyces; genetics; metabolism; molecular model; X ray crystallography ⓘ English 2025 2025-02 10.3390/ijms26041661 ⓘ 바로가기 바로가기 바로가기 바로가기
○ ○ Article ⓘ Rubia akane Nakai Fruit Extract Improves Obesity and Insulin Sensitivity in 3T3-L1 Adipocytes and High-Fat Diet-Induced Obese Mice ⓘ A rise in obesity during the COVID-19 pandemic has spurred the development of safe and effective natural anti-obesity agents. In this study, we propose Rubia akane Nakai fruit extract (RFE) as a potential natural product-based anti-obesity agent. R. akane Nakai is a plant of the Rubiaceae family that grows throughout Republic of Korea. Its roots have long been used medicinally and are known for various bioactivities, but the fruit's bioactivities are unexplored. We investigated the anti-obesity effects of RFE using 3T3-L1 adipocytes and high-fat diet-induced obese mice. In 3T3-L1 adipocytes, RFE inhibited adipogenic differentiation and lipogenesis by downregulating PPAR gamma (peroxisome proliferator-activated receptor gamma), C/EBP alpha (CCAAT enhancer-binding protein alpha), and SREBP-1 (sterol regulatory element-binding protein 1) through AMPK (AMP-activated protein kinase) activation and by delaying the initiation of MCE (mitotic clonal expansion), which is essential for early adipogenesis. At the in vivo level, RFE improved the phenotypes of obesity and insulin resistance. In white adipose tissue, RFE not only suppressed adipogenic differentiation and lipogenesis through AMPK activation but also improved insulin sensitivity by upregulating basal GLUT4 (glucose transporter type 4) expression. Therefore, this study advances RFE as a potential natural treatment for obesity and insulin resistance. ⓘ Park, Juhye; Lee, Eunbi; Nam, Ju-Ock ⓘ Kyungpook Natl Univ, Coll Agr & Life Sci, Sch Food Sci & Biotechnol, Daegu 41566, South Korea; Kyungpook Natl Univ, Res Inst Tailored Food Technol, Daegu 41566, South Korea ⓘ ⓘ 58508479500; 57798039800; 7201496105 ⓘ pdw8609@knu.ac.kr; 21eunbi@knu.ac.kr; namjo@knu.ac.kr; ⓘ INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES ⓘ INT J MOL SCI ⓘ 1661-6596 1422-0067 26 5 SCIE BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY ⓘ 2024 4.9 22.4 0 2025-05-07 ⓘ 0 0 Rubia akane Nakai fruit; anti-obesity; adipogenic differentiation; lipogenesis; insulin sensitivity; AMPK ⓘ CELLS ⓘ adipogenic differentiation; AMPK; anti-obesity; insulin sensitivity; lipogenesis; Rubia akane Nakai fruit ⓘ 3T3-L1 Cells; Adipocytes; Adipogenesis; Animals; Anti-Obesity Agents; CCAAT-Enhancer-Binding Protein-alpha; Cell Differentiation; Diet, High-Fat; Fruit; Insulin Resistance; Lipogenesis; Male; Mice; Mice, Inbred C57BL; Mice, Obese; Obesity; Plant Extracts; PPAR gamma; Sterol Regulatory Element Binding Protein 1; antiobesity agent; CCAAT enhancer binding protein alpha; glucose transporter 4; hydroxymethylglutaryl coenzyme A reductase kinase; peroxisome proliferator activated receptor gamma; plant extract; Rubia akane extract; rutoside; sterol regulatory element binding protein 1; unclassified drug; antiobesity agent; CCAAT enhancer binding protein alpha; peroxisome proliferator activated receptor gamma; plant extract; sterol regulatory element binding protein 1; 3T3-L1 cell line; adipogenesis; AMPK signaling; animal cell; animal experiment; animal model; animal tissue; antiobesity activity; Article; cell differentiation; controlled study; diet-induced obesity; fruit; high performance liquid chromatography; in vivo study; insulin resistance; insulin sensitivity; lipogenesis; liquid chromatography-mass spectrometry; male; mouse; mouse mutant; nonhuman; phenotype; proadipocyte; protein expression level; receptor down regulation; Rubiaceae; upregulation; white adipose tissue; 3T3-L1 cell line; adipocyte; adipogenesis; adverse event; animal; C57BL mouse; chemistry; drug effect; drug therapy; etiology; fruit; genetics; insulin resistance; lipid diet; metabolism; obesity ⓘ English 2025 2025-03 10.3390/ijms26051833 ⓘ 바로가기 바로가기 바로가기 바로가기
○ ○ Article ⓘ The First Complete Chloroplast Genome of Spider Flower (Cleome houtteana) Providing a Genetic Resource for Understanding Cleomaceae Evolution ⓘ In the present study, the sequencing and analysis of the complete chloroplast genome of Cleome houtteana and its comparison with related species in the Cleomaceae family were carried out. The genome spans 157,714 base pairs (bp) and follows the typical chloroplast structure, consisting of a large single-copy (LSC) region (87,506 bp), a small single-copy (SSC) region (18,598 bp), and two inverted repeats (IRs) (25,805 bp each). We identified a total of 129 genes, including 84 protein-coding genes, 8 ribosomal RNA (rRNA) genes, and 37 transfer RNA (tRNA) genes. Our analysis of simple sequence repeats (SSRs) and repetitive elements revealed 91 SSRs, with a high number of A/T-rich mononucleotide repeats, which are common in chloroplast genomes. We also observed forward, palindromic, and tandem repeats, which are known to play roles in genome stability and evolution. When comparing C. houtteana with its relatives, we identified several highly variable regions, including ycf1, ycf2, and trnH-psbA, marking them as propitious molecular markers for the identification of species as well as phylogenetic studies. We examined the inverted repeat (IR) boundaries and found minor shifts in comparison to the other species, particularly in the ycf1 gene region, which is a known hotspot for evolutionary changes. Additionally, our analysis of selective pressures (Ka/Ks ratios) showed that most genes are under strong purifying selection, preserving their essential functions. A sliding window analysis of nucleotide diversity (Pi) identified several regions with high variability, such as trnH-psbA, ycf1, ndhI-ndhG, and trnL-ndhF, highlighting their potential for use in evolutionary and population studies. Finally, our phylogenetic analysis, using complete chloroplast genomes from species within Cleomaceae, Brassicaceae, and Capparaceae, confirmed that C. houtteana belongs within the Cleomaceae family. It showed a close evolutionary relationship with Tarenaya hassleriana and Sieruela rutidosperma, supporting previous taxonomic classifications. The findings from the current research offer invaluable insights regarding genomic structure, evolutionary adaptations, and phylogenetic relationships of C. houtteana, providing a foundation for future research on species evolution, taxonomy, and conservation within the Cleomaceae family. ⓘ Lubna, Rahmatullah; Jan, Rahmatullah; Hashmi, Syed Salman; Asif, Saleem; Bilal, Saqib; Waqas, Muhammad; Abdelbacki, Ashraf M. M.; Kim, Kyung-Min; Al-Harrasi, Ahmed; Asaf, Sajjad ⓘ Univ Nizwa, Nat & Med Sci Res Ctr, Nizwa 616, Oman; Kyungpook Natl Univ, Coastal Agr Res Inst, Daegu 41566, South Korea; Kyungpook Natl Univ, Dept Appl Biosci, Daegu 41566, South Korea; Govt Khyber Pakhtunkhwa, Dept Agr Extens, Mardan 23200, Pakistan; King Saud Univ, Deanship Skills Dev, POB 2455, Riyadh 11451, Saudi Arabia ⓘ Jan, Rahmatullah/AIC-3439-2022; Abdelbacki, Dr. Ashraf/LYO-2547-2024; Ul-Hamid, Anwar/B-7297-2015; Asaf, Sajjad/ABA-3647-2021 ⓘ 59658046000; 57201981969; 57202092345; 57396413700; 57031617400; 59139606000; 35723565200; 34868260300; 6506093146; 56595059900 ⓘ rehmatbot@yahoo.com; sshashmi10@gmail.com; agronomist89@gmail.com; kkm@knu.ac.kr; sajadasif2000@gmail.com; ⓘ INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES ⓘ INT J MOL SCI ⓘ 1661-6596 1422-0067 26 8 SCIE BIOCHEMISTRY & MOLECULAR BIOLOGY;CHEMISTRY, MULTIDISCIPLINARY ⓘ 2024 4.9 22.4 0 2025-05-07 ⓘ 0 0 chloroplast genome; SSRs; Ycf1 gene; phylogenetic analysis; repetitive elements; inverted repeat boundaries; nucleotide diversity; selective pressure ⓘ BRASSICACEAE; ORGANIZATION; PHYLOGENY; SOFTWARE; PROGRAM ⓘ chloroplast genome; inverted repeat boundaries; nucleotide diversity; phylogenetic analysis; repetitive elements; selective pressure; SSRs; Ycf1 gene ⓘ Chloroplasts; Evolution, Molecular; Genome, Chloroplast; Microsatellite Repeats; Phylogeny; molecular marker; ribosome RNA; transfer RNA; microsatellite DNA; Article; Brassicaceae; Capparaceae; chloroplast; chloroplast genome; Cleomaceae; Cleome; Cleome houtteana; evolutionary adaptation; flower; genetic resource; genomic instability; inverted repeat; nonhuman; phylogeny; purifying selection; simple sequence repeat; tandem repeat; genetics; molecular evolution ⓘ English 2025 2025-04-09 10.3390/ijms26083527 ⓘ 바로가기 바로가기 바로가기 바로가기
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