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○ | ○ | Article | Efficacy of ossiculoplasty in patients with chronic otitis media with severe to profound hearing loss | Chronic otitis media (COM) is treated medically and/or surgically. Ossiculoplasty for COM with severe-to-profound hearing loss has not been recommended because the severity of hearing loss before surgery is negatively associated with postoperative hearing improvement. Our study assessed the effectiveness of ossiculoplasty for severe-to-profound hearing loss and identified prognostic factors associated with favorable outcomes. We retrospectively reviewed the medical records of 182 patients with severe-to-profound hearing loss who underwent ossiculoplasty with tympanoplasty and mastoidectomy for COM between January 2017 and December 2018. Air conduction, bone conduction, and air-bone gap significantly improved compared to baseline values. Univariate analysis revealed that high ossiculoplasty outcome parameter staging (OOPS) or middle ear risk index (MERI) scores, obstructed Eustachian tube status, or computed tomography (CT) findings with sclerotic mastoid or soft tissue density (STD) of the retrotympanic space were associated with a lower odds ratio for air gain >= 20 dB at 12 months postoperatively. Multivariate analysis revealed that a high OOPS score and obstructed Eustachian tube significantly predicted poorer outcomes. In conclusion, ossiculoplasty can be considered for selected patients with COM, such as those with a low OOPS index, aerated middle ear, or no STD of the retrotympanic space on CT, before cochlear implantation even if they had severe-to-profound hearing loss and high bone conduction threshold. | Jung, Da Jung; Son, Jigon; Kwon, Ha Gyung; Kwon, Eunhye; Lee, Kyu-Yup | Kyungpook Natl Univ, Sch Med, Dept Otorhinolaryngol Head & Neck Surg, 200 Dongdeok Ro, Daegu 700721, South Korea | 57220970862; 59399383700; 58785879200; 59334656400; 22135779500 | kylee@knu.ac.kr; | SCIENTIFIC REPORTS | SCI REP-UK | 2045-2322 | 15 | 1 | SCIE | MULTIDISCIPLINARY SCIENCES | 2024 | 3.9 | 18.1 | 0 | 2025-05-07 | 0 | 0 | Chronic otitis media; Ossiculoplasty; Profound hearing loss | PROGNOSTIC-FACTORS; MIDDLE-EAR; BONE; PREDICTION | Chronic otitis media; Ossiculoplasty; Profound hearing loss | Adolescent; Adult; Aged; Child; Chronic Disease; Female; Hearing Loss; Humans; Male; Mastoidectomy; Middle Aged; Otitis Media; Retrospective Studies; Severity of Illness Index; Treatment Outcome; Tympanoplasty; Young Adult; adolescent; adult; aged; child; chronic disease; female; hearing impairment; human; male; mastoidectomy; middle aged; otitis media; procedures; retrospective study; severity of illness index; surgery; treatment outcome; tympanoplasty; young adult | English | 2025 | 2025-02-10 | 10.1038/s41598-024-84966-0 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |||
○ | ○ | Article | Foliar application of nano biochar solution elevates tomato productivity by counteracting the effect of salt stress insights into morphological physiological and biochemical indices | Nano-biochar considers a versatile and valuable sorbent to enhance plant productivity by improving soil environment and emerged as a novel solution for environmental remediation and sustainable agriculture in modern era. In this study, roles of foliar applied nanobiochar colloidal solution (NBS) on salt stressed tomato plants were investigated. For this purpose, NBS was applied (0%, 1% 3% and 5%) on two groups of plants (control 0 mM and salt stress 60 mM). Tween-20 was used as a surfactant to prolong NBS effective stay on plant leaf surface. The results showed that 3% NBS application effectively improved the plant height, plant biomass, fruit count and fruit weight under non-stressed and stressed plants. In addition, 3% NBS application further increased the plant pigments such as chlorophyll by 72% and 53%, carotenoids by 64% and 40%, leaf relative water content by 4.1 fold and 1.07 fold under both conditions, respectively. NBS application stabilized the plasma membrane via reducing electrolyte leakage by 30% as well as reduced the lipid peroxidation rates by 46% and 29% under non-stressed and stressed plants, respectively. 3% NBS application also significantly enhanced the plants primary and secondary metabolites, as well as activities of antioxidant enzymes compared to control plants. Overall, NBS foliar application significantly improved all growth and yield indices, pigments, primary and secondary metabolites, leaf water content, antioxidant enzyme activities as well as reduced electrolyte leakage and lipid peroxidation rates in tomato to combat stress conditions. In future, studies on nano biochar interactions with soil microbiota, surface modifications, long-term environmental impacts, reduced methane gas emissions, and biocompatibility could provide insights into optimizing its use in sustainable agriculture. | Shahzadi, Javeria; Zaib-un-Nisa, Naila; Ali, Naila; Iftikhar, Muhammad; Shah, Anis Ali; Ashraf, M. Yasin; Chao, Chen; Shaffique, Shifa; Gatasheh, Mansour K. | Univ Lahore, Inst Mol Biol & Biotechnol, Lahore, Pakistan; Univ Educ, Dept Bot, Div Sci & Technol, Lahore 54770, Pakistan; Harbin Normal Univ, Sch Life Sci & Technol, Dept Chem & Mol Biol, Harbin 150025, Peoples R China; Kyungpook Natl Univ, Coll Agr & Life Sci, Sch Appl Biosci, 80 Daehak Ro, Daegu 41566, South Korea; King Saud Univ, Coll Sci, Dept Biochem, POB 2455, Riyadh 11451, Saudi Arabia | Gatasheh, Mansour/AAP-9414-2021; Shah, Dr. Anis/AAV-2232-2020; shaffique, shifa/KUC-7102-2024; Iftikhar, Muhammad/KGM-4844-2024 | 57470190100; 59156412200; 57792045200; 59875899600; 57211606140; 59567542300; 57221732806; 57203898867; 15742571400 | zaib.nisa@imbb.uol.edu.pk; anisalibot@gmail.com; | SCIENTIFIC REPORTS | SCI REP-UK | 2045-2322 | 15 | 1 | SCIE | MULTIDISCIPLINARY SCIENCES | 2024 | 3.9 | 18.1 | 0 | 2025-05-07 | 2 | 2 | Nanobiochar; Biochemical indices; Growth; Yield; Metabolites; Antioxidant enzymes | OXIDATIVE DAMAGE; WATER-STRESS; NANOPARTICLES; TRANSPORT; SALINITY; GROWTH; LEAVES; SOIL; CHLOROPLASTS; SENESCENCE | Antioxidant enzymes; Biochemical indices; Growth; Metabolites; Nanobiochar; Yield | Antioxidants; Biomass; Carotenoids; Charcoal; Chlorophyll; Lipid Peroxidation; Nanoparticles; Plant Leaves; Salt Stress; Solanum lycopersicum; antioxidant; carotenoid; charcoal; chlorophyll; nanoparticle; biomass; chemistry; drug effect; growth, development and aging; lipid peroxidation; metabolism; plant leaf; salt stress; tomato | English | 2025 | 2025-01-25 | 10.1038/s41598-025-87399-5 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||
○ | ○ | Article | Green synthesized FeNPs ameliorate drought stress in Spinacia oleracea L. through improved photosynthetic capacity, redox balance, and antioxidant defense | The present study was designed to highlight the ameliorative role of iron nanoparticles (FeNPs) against drought stress in spinach (Spinacia oleracea L.) plants. A pot experiment was performed in two-way completely randomize design with three replicates. For drought stress three levels were used by maintaining field capacity of the soil. This included control (100% field capacity), moderate drought stress (D1; 50% field capacity) and severe drought stress (D2; 25% field capacity). FeNPs synthesized by green method using rice straw were applied along with precursor FeCl3, used as Fe source for the synthesis of FeNPs, through foliar spray (40 mg L- 1 for both). Growth parameters, efficiency of photosynthetic machinery, gas exchange attributes, total soluble proteins, and inorganic ions (Ca2+, K+ & Fe2+) were significantly reduced at both D1 and D2 stress levels, compared to control plants. Fe supplements in the form of FeCl3 and FeNPs improved these attributes in both control and drought conditions. Malondialdehyde, H2O2, relative membrane permeability (stress indicators) and the activities of antioxidants were increased in response to drought stress. Fe supplements further improved the antioxidant defense activities and efficiently lowered the effects of stress indicators. These effects of FeCl3 and FeNPs resulted in improved growth of S. oleracea plants in control and drought conditions. Results showed that FeNPs had more prominent effects on growth of S. oleracea plants compared to FeCl3. These findings suggest that FeNPs could be a helpful tool for lessening the harmful consequences of drought stress and this can be used for abiotic stress alleviation in other crops as well. | Naseem, Javeria; Shah, Anis Ali; Usman, Sheeraz; Ahmed, Shakil; Gatasheh, Mansour K.; Shaffique, Shifa; Javad, Sumera | Univ Educ, Dept Bot, Div Sci & Technol, Lahore, Pakistan; Univ Punjab, Dept Bot, Lahore, Pakistan; King Saud Univ, Coll Sci, Dept Biochem, POB 2455, Riyadh 11451, Saudi Arabia; Kyungpook Natl Univ, Sch Appl Biosci, Daegu 41566, South Korea; Lahore Coll Women Univ, Dept Bot, Lahore, Pakistan | Shah, Dr. Anis/AAV-2232-2020; shaffique, shifa/KUC-7102-2024; Gatasheh, Mansour/AAP-9414-2021; Usman, Sheeraz/KZU-1594-2024; Javad, Sumera/ADB-7547-2022 | 59334693300; 57211606140; 58199413800; 57209192993; 15742571400; 57203898867; 35344711200 | anisalibot@gmail.com; miansheerazusman@gmail.com; | SCIENTIFIC REPORTS | SCI REP-UK | 2045-2322 | 15 | 1 | SCIE | MULTIDISCIPLINARY SCIENCES | 2024 | 3.9 | 18.1 | 0 | 2025-05-07 | 2 | 4 | Abiotic stress; Growth; Nanoparticles; Photosynthetic machinery; Stress indicators | OXIDATIVE STRESS; PLANT-GROWTH; NANOPARTICLES; RESPONSES; PEROXIDASE; TOLERANCE; PROLINE | Abiotic stress; Growth; Nanoparticles; Photosynthetic machinery; Stress indicators | Antioxidants; Droughts; Iron; Metal Nanoparticles; Oxidation-Reduction; Photosynthesis; Spinacia oleracea; Stress, Physiological; antioxidant; iron; metal nanoparticle; chemistry; drought; drug effect; metabolism; oxidation reduction reaction; photosynthesis; physiological stress; spinach | English | 2025 | 2025-01-13 | 10.1038/s41598-024-84061-4 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||
○ | ○ | Article | Hybridization and diversity of the genus Vandenboschia in Korea insights from morphological, cytological, and genotype analyses | The genus Vandenboschia exhibits significant diversity, driven by complex hybridization events and varying ploidy levels in the natural habitats of Korea, leads to misidentifications that obscure its true distribution and classification. To address this issue, the present study employed morphological, cytological, and genotype analyses to clarify the taxonomical circumscription and distribution patterns of the Vandenboschia radicans complex in Korea. The V. radicans complex in the genus refers to V. radicans and its closely related species and exhibits significant morphological variation, making classification challenging. Previous molecular studies have revealed that it consists of several non-hybrid and hybrid species among them. Through the present study, comprehensive sampling from Jeju and Ulleung Islands identified five distinct taxa from the V. radicans complex: two non-hybrid species (Vandenboschia kalamocarpa and Vandenboschia nipponica), and three hybrids (Vandenboschia x stenosiphon, Vandenboschia x quelpaertensis, and Vandenboschia kalamocarpa x Vandenboschia nipponica x Vandenboschia striata). Notably, hybrids exhibited broader geographic distributions compared to non-hybrid species, which were confined to specific microhabitats. While overlapping morphological traits among the species complicate identification without genetic analysis, rhizome diameter and the width-to-length ratio of the involucre can serve as key morphological traits for distinguishing members of the Vandenboschia radicans complex in Korea. Additionally, cleaved amplified polymorphic sequence (CAPS) markers and cloning techniques were employed for species identification, particularly when morphological traits were inconclusive. These findings highlight the need to integrate genetic tools with traditional taxonomic methods to resolve complex species relationships within Vandenboschia in Korea and emphasize the importance of conservation efforts for geographically restricted and genetically distinct populations. | Park, Sang Hee; Kim, Jung Sung; Kim, Hyoung Tae | Chungbuk Natl Univ, Dept Forest Sci, Cheongju 28644, Chungbuk, South Korea; Kyungpook Natl Univ, Dept Crop Sci, Sangju 37224, South Korea | 57217737985; 55227125400; 56181017100 | jungsung@cbnu.ac.kr; htkim0922@knu.ac.kr; | SCIENTIFIC REPORTS | SCI REP-UK | 2045-2322 | 15 | 1 | SCIE | MULTIDISCIPLINARY SCIENCES | 2024 | 3.9 | 18.1 | 0 | 2025-05-07 | 0 | 0 | Vandenboschia radicans complex; CAPS; Genotype; Morphology; Hybrid; Ploidy | RADICANS COMPLEX HYMENOPHYLLACEAE; CHLOROPLAST DNA; NUCLEAR-DNA; GAMETOPHYTES | CAPS; Genotype; Hybrid; Morphology; Ploidy; Vandenboschia radicans complex | Genetic Variation; Genotype; Hybridization, Genetic; Phylogeny; Republic of Korea; genetic variation; genotype; hybridization; phylogeny; South Korea | English | 2025 | 2025-01-10 | 10.1038/s41598-025-86000-3 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |||
○ | ○ | Article | Low-profile conformal single-sided miniaturized frequency selective surface for wideband shielding | This paper presents an ultra-wideband (UWB) frequency selective surface (FSS) that is conformal, low-profile, single-layered, and miniaturized. The designed simple modified star swastika (MSS) arms offer a wide bandwidth of 7.5 GHz with transmission nulls ranging from 3.1 to 10.6 GHz. The 0.0008 lambda 0 thin MSS FSS unit cells form a periodic pattern with a physical form factor of 0.041 lambda 0 and 61% miniaturization. The proposed FSS has a -10 dB fractional bandwidth of 109.48% and a peak shielding of 67.9 dB at 7.1 GHz. The designed FSS has a figure of merit (FoM) of 10.25 (lambda 0/U), where U represents the unit cell size of the FSS. In both transverse electric (TE) and transverse magnetic (TM) modes, incoming waves can be stable with incidence angles (theta) up to 75 degrees and less than 1% frequency deviation. To gain a physical understanding of the FSS unit cell, an equivalent circuit model (ECM) analysis is conducted. The FSS prototype has been built, and the measured results are validated with simulated results. Hence, the proposed MSS FSS could be a promising solution for wideband shielding. | Kanagasabai, Malathi; Viswanathan, Lavanya; Alsath, M. Gulam Nabi; Palaniswamy, Sandeep Kumar; Kumar, Sachin; Premkumar, Santhya Premdharshini; Choi, Hyun Chul; Kim, Kang Wook | Anna Univ, Coll Engn, Dept Elect & Commun Engn, Chennai 600025, India; SRM Inst Sci & Technol, Fac Engn & Technol, Dept Elect & Commun, Kattankulathur 603203, India; Galgotias Coll Engn & Technol, Dept Elect & Commun Engn, Greater Noida 201310, India; Kyungpook Natl Univ, Sch Elect & Elect Engn, Daegu 41566, South Korea | Kumar, Sachin/W-2211-2019; Kanagasabai, Malathi/ABH-5331-2020; Palaniswamy, Sandeep Kumar/AAF-2240-2021; Alsath, M., Gulam/ADX-0504-2022 | 55511179300; 58823057000; 54580566800; 56158830800; 56907994000; 59149463700; 57193342681; 57204432422 | vrpchs@gmail.com; kang_kim@ee.knu.ac.kr; | SCIENTIFIC REPORTS | SCI REP-UK | 2045-2322 | 15 | 1 | SCIE | MULTIDISCIPLINARY SCIENCES | 2024 | 3.9 | 18.1 | 0 | 2025-05-07 | 0 | 0 | DESIGN | article; bandwidth; cell size; controlled study; human; miniaturization; simulation | English | 2025 | 2025-04-05 | 10.1038/s41598-025-92421-x | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||||
○ | ○ | Article | Machine learning-based pattern recognition of Bender element signals for predicting sand particle-size | This study explores the potential of integrating bender element signals with a convolutional neural network (CNN) to predict the particle size distribution of relatively uniform sand. A one-dimensional CNN analyzed time-series signals from bender elements across four sand types with particle sizes ranging from 0.5 to approximately 7 mm, under vertical stresses of 10, 50, and 150 kPa in three different cutoff frequencies (10, 50, and 100 kHz). The CNN architecture included convolutional layers augmented with batch normalization and ReLU activation functions, optimized through Bayesian techniques to enhance prediction accuracy. Experimental results demonstrated that higher stresses increased resonant frequencies and reduced arrival times of shear waves, with minor dependencies on soil type. Nevertheless, the developed CNN model well classified the four sand types at a given vertical stress and cutoff frequency, implying that the unique pattern of each sand type can be satisfactorily captured by the CNN algorithm. Overall, the framework shown in this study demonstrates that the bender element (or pattern of receiving shear wave signals) with the CNN model can be used in monitoring real-time variation of sand particle size. | Byun, Yong-Hoon; Son, Juik; Yun, Jungmin; Choo, Hyunwook; Won, Jongmuk | Kyungpook Natl Univ, Dept Agr Civil Engn, 80 Daehak Ro, Daegu 41566, South Korea; Univ Ulsan, Dept Civil & Environm Engn, Daehak Ro 93, Ulsan 680749, South Korea; Hanyang Univ, Dept Civil & Environm Engn, Seoul 04763, South Korea; Ulsan Natl Inst Sci & Technol UNIST, Dept Civil Earth & Environm Engn, UNIST Gil 50, Ulsan 44919, South Korea | Byun, Yong-Hoon/JKI-8441-2023 | 42761048000; 59654967300; 59377326500; 35365797900; 53878877200 | jwon@unist.ac.kr; | SCIENTIFIC REPORTS | SCI REP-UK | 2045-2322 | 15 | 1 | SCIE | MULTIDISCIPLINARY SCIENCES | 2024 | 3.9 | 18.1 | 0 | 2025-05-07 | 2 | 2 | Bender element; Convolutional neural network; Vertical stress; Cutoff frequency; Sand particle size | CONVOLUTIONAL NEURAL-NETWORK; SHEAR-WAVE VELOCITY; STIFFNESS CHARACTERISTICS; HYDRAULIC CONDUCTIVITY; RELATIVE DENSITY; GRAIN-SIZE; STRENGTH; LIQUEFACTION; BEHAVIOR; MODULUS | Bender element; Convolutional neural network; Cutoff frequency; Sand particle size; Vertical stress | algorithm; article; convolutional neural network; diagnosis; electric potential; machine learning; male; particle size; pattern recognition; physiological stress; prediction; sand; time series analysis | English | 2025 | 2025-02-26 | 10.1038/s41598-025-91497-9 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||
○ | ○ | Article | Many objective optimization and decision support for dairy cattle feed formulation | Livestock feed formulation has significant impacts on livestock production and the environment. Linear and nonlinear constraints, framed as nutritional requirements and specific objectives, present a continuous challenge in achieving optimal feed formulation. Many mathematical models, including linear programming, have been adopted to tackle this issue. However, this approach is often excessively restrictive, primarily focusing on cost minimization and overlooking variability in nutrient content and fulfillment of other objectives. Conventional feed formulation approaches, characterized by their limited operational scope and inadequacy for a robust decision-making process, present challenges to growers aiming to achieve objectives beyond cost minimization. This study proposes a many-objective optimization approach to solving the feed formulation problem and addressing this challenge. The framework optimizes nine objectives, including minimizing cost, weight and the number of feed components, and five nutritional constraints. By integrating feed nutritional constraints and objectives into a comprehensive framework, we aim to introduce flexibility and enhance decision-making. The proposed framework successfully balances the nine objectives, providing growers with a potentially adaptable and tailored solutions. Growers can achieve trade-offs across various objectives, enabling informed decision-making to optimize feed formulation, enhance livestock productivity, and promote environmental sustainability. Furthermore, visualization tools were utilized to improve the interpretability of the generated solutions. The results obtained demonstrate acceptable compromise across various objectives. | Usigbe, Member Joy; Uyeh, Daniel Dooyum; Park, Tusan; Ha, Yushin; Mallipeddi, Rammohan | Kyungpook Natl Univ, Sch Elect Engn, Dept Artificial Intelligence, Daegu 41566, South Korea; Michigan State Univ, Dept Biosyst & Agr Engn, E Lansing, MI 48824 USA; Kyungpook Natl Univ, Smart Agr Innovat Ctr, Daegu 41566, South Korea; Kyungpook Natl Univ, Dept Smart Bioind Mech Engn, Daegu 41566, South Korea; Kyungpook Natl Univ, Upland Field Machinery Res Ctr, Daegu 41566, South Korea; Kyungpook Natl Univ, Dept Appl Biosci, Daegu 41566, South Korea | ; Park, Tusan/C-2057-2012; Mallipeddi, Rammohan/AAL-5306-2020 | 58512627500; 57194449611; 57202780408; 57192072314; 25639919900 | yushin72@knu.ac.kr; mallipeddi@knu.ac.kr; | SCIENTIFIC REPORTS | SCI REP-UK | 2045-2322 | 15 | 1 | SCIE | MULTIDISCIPLINARY SCIENCES | 2024 | 3.9 | 18.1 | 0 | 2025-05-07 | 0 | 0 | Many objective optimization; Nutritrient balance; Resource optimization; Production efficiency; Grower flexibility | Grower flexibility; Many objective optimization; Nutritrient balance; Production efficiency; Resource optimization | Animal Feed; Animal Nutritional Physiological Phenomena; Animals; Cattle; Dairying; Decision Support Techniques; Models, Theoretical; animal; animal food; bovine; dairying; decision support system; procedures; theoretical model | English | 2025 | 2025-04-18 | 10.1038/s41598-025-96633-z | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |||
○ | ○ | Article | Mitigation of adverse effect of cadmium toxicity in lettuce (Lactuca sativa L.) through foliar application of chitosan and spermidine | Cadmium (Cd) stress is considered among the most harmful abiotic stresses because of its toxicity and ability to alter the ultrastructure of plants. Lettuce (Lactuca sativa L.) can readily accumulate Cd from the soil, but its elevated level posed negative effect on their development and nutritional quality. In this study, efficacy of chitosan and spermidine synergistic application was evaluated to improve Cd metal tolerance or its exclusion in lettuce. A pot experiment was conducted in a four-way completely randomized design (CRD) with 3 replicates, using two L. sativa varieties (VRIL-0205 and Green Check). Following treatments, Cd stress (10 ppm CdCl2), chitosan (200 ppm) and spermidine (145 ppm) were applied along with their respective controls. The negative effects of Cd stress on the morphological, physiological, and biochemical attributes of both L. sativa varieties were evaluated along with counter effect of chitosan and spermidine alone and synergistic application. Cd stress resulted in significant accumulation of Cd2+ ions in the shoot of both varieties (0.038 mg kg- 1 in VRIL-0205 and 0.041 mg kg- 1 in Green Check). It also impaired growth, biomass, gas exchange, water relation, antioxidant activities and nutrient uptake in both varieties. Foliar application of both chitosan and spermidine improved growth, biomass, chlorophyll content, photosynthesis rate, stomatal conductance, water content, antioxidant activities and nutrient uptake in both control and stressed plants. Their combined treatment reduced stress indicators including relative membrane permeability (VRIL-0205; 19% and Green Check; 22%), H2O2 (VRIL-0205; 27% and Green Check; 26%) and malondialdehyde content (VRIL-0205; 6% and Green Check; 7%) in stressed plants, compared with stress only plants. These findings showed that chitosan and spermidine synergistic application effectively mitigated the Cd toxicity in both L. sativa varieties and improved their growth under stress condition. This study provides insight into the potential use of chitosan and spermidine foliar spray as sustainable tools for improving Cd resilience in crop plants. | Rafique, Maham; Noreen, Zahra; Usman, Sheeraz; Shah, Anis Ali; Taj, Hafsa; El-Sheikh, Mohamed A.; Lee, In Jung | Univ Educ, Dept Bot, Div Sci & Technol, Lahore, Pakistan; King Saud Univ, Coll Sci, Bot & Microbiol Dept, Riyadh, Saudi Arabia; Kyungpook Natl Univ, Coll Agr & Life Sci, Sch Appl Biosci, 80 Daehak Ro, Daegu 41566, South Korea | Noreen, Zahra/G-4701-2011; Shah, Dr. Anis/AAV-2232-2020; Usman, Sheeraz/KZU-1594-2024; El-sheikh, Mohamed/AAO-4652-2020; Lee, In-Jung/GLS-0432-2022 | 59401905900; 24462254900; 58199413800; 57211606140; 59401874100; 8429327500; 16425830900 | zahranoreen@gmail.com; miansheerazusman@gmail.com; anisalibot@gmail.com; ijlee@knu.ac.kr; | SCIENTIFIC REPORTS | SCI REP-UK | 2045-2322 | 15 | 1 | SCIE | MULTIDISCIPLINARY SCIENCES | 2024 | 3.9 | 18.1 | 0 | 2025-05-07 | 0 | 0 | Abiotic stress; Antioxidants; Heavy metals; Nutrient imbalance; Stress resilience; Sustainable tool | EXOGENOUS APPLICATION; LIPID-PEROXIDATION; WHEAT SEEDLINGS; NITRIC-OXIDE; CD STRESS; ANTIOXIDANT; GROWTH; WATER; POLYAMINES; SYSTEM | Abiotic stress; Antioxidants; Heavy metals; Nutrient imbalance; Stress resilience; Sustainable tool | Antioxidants; Cadmium; Chitosan; Chlorophyll; Lactuca; Photosynthesis; Plant Leaves; Soil Pollutants; Spermidine; Stress, Physiological; antioxidant; cadmium; chitosan; chlorophyll; spermidine; drug effect; growth, development and aging; Lactuca; metabolism; photosynthesis; physiological stress; plant leaf; soil pollutant; toxicity | English | 2025 | 2025-03-17 | 10.1038/s41598-025-93672-4 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||
○ | ○ | Article | Naphthalene acetic acid induced morphological and biochemical alterations in Lagenaria siceraria under alkaline stress | Alkaline stress exacerbates ionic toxicity, leading to a decline in plant growth and yield. Lagenaria siceraria (Molina) Stndl., commonly known as squash, an annual plant belonging to the Cucurbitaceae family, is sensitive to alkalinity because of its impact on physiological growth and yield of plant. However, studies on the utilization of plant growth regulators to alleviate alkali stress in Lagenaria siceraria is scarce. In the present study, a pot experiment was conducted to evaluate the impact of foliar application of NAA (0, 50, 75, or 100 ppm) on the growth, yield, and biochemical parameters of L. siceraria under both normal and alkaline stress conditions (0 and 40 mM). The findings revealed that alkaline stress significantly diminished plant growth, biomass, and leaf and fruit counts, whereas NAA application amplified all growth and yield characteristics under both stressful and normal conditions. Additionally, compared with salt stress alone, alkaline stress markedly decreased the levels of photosynthetic pigments; however, 75% NAA application resulted in 43% increase in Chl a, 53% increase in Chl b, and 66% increase in carotenoids. Moreover, there was a notable increase in the primary and secondary metabolites of plants treated with NAA, including a 27% increase in total soluble proteins (TSP), a 38% increase in total free amino acids (TFA), and a 28% and 27% increase in total phenolic and flavonoid contents, respectively, in comparison with the plants subjected only to stress. To further explore the impact of NAA on biochemical parameters of L. siceraria, antioxidant enzymes like catalase (CAT) and peroxidase (POD) activities were assessed. The results indicated that alkali stress increased enzyme activities, which were further increased under the influence of foliar-applied NAA as compared to the plants exposed solely to stress. These findings underscore the beneficial role of exogenous NAA application in mitigating the adverse effects of alkali stress on L. siceraria. | Nadeem, Uswa Ashiq; Ali, Naila; Abbas, Toqeer; Shah, Anis Ali; Arif, Muhammad; Gatasheh, Mansour K.; Shaffique, Shifa | Univ Lahore, Inst Mol Biol & Biotechnol, Lahore, Pakistan; Univ Sargodha, Dept Bot, Sargodha, Pakistan; Univ Educ, Dept Bot, Div Sci & Technol, Lahore, Punjab, Pakistan; Univ Sargodha, Coll Agr, Dept Anim Sci, Sargodha, Pakistan; King Saud Univ, Coll Sci, Dept Biochem, POB 2455, Riyadh 11451, Saudi Arabia; Kyungpook Natl Univ, Coll Agr & Life Sci, Sch Appl Biosci, 80 Daehak Ro, Daegu 41566, South Korea | Arif, Muhammad/P-2958-2019; Abbas, Toqeer/KHY-4557-2024; Gatasheh, Mansour/AAP-9414-2021; Shah, Dr. Anis/AAV-2232-2020; shaffique, shifa/KUC-7102-2024 | 59367055300; 57792045200; 59156412200; 57202833813; 57211606140; 58822065200; 15742571400; 57203898867 | naila.ali@imbb.uol.edu.pk; toqeerabbas01@gmail.com; | SCIENTIFIC REPORTS | SCI REP-UK | 2045-2322 | 15 | 1 | SCIE | MULTIDISCIPLINARY SCIENCES | 2024 | 3.9 | 18.1 | N/A | 0 | 0 | Vegetables; High pH; Growth regulators; Pigments; Metabolites; Catalase; Peroxidase | SALT STRESS; DEFENSE | Catalase; Growth regulators; High pH; Metabolites; Peroxidase; Pigments; Vegetables | Biomass; Cucurbitaceae; Photosynthesis; Plant Growth Regulators; Plant Leaves; Stress, Physiological; phytohormone; biomass; Cucurbitaceae; drug effect; growth, development and aging; metabolism; photosynthesis; physiological stress; plant leaf | English | 2025 | 2025-05-06 | 10.1038/s41598-025-99455-1 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |||
○ | ○ | Article | Neuromuscular dynamics during isometric knee contractions: effects of target force, knee angle, and tibial rotation on force steadiness | This study investigated neuromuscular variations across low to moderate target force levels, three knee joint angles and tibial rotation positions, examining their correlations with muscle morphology and their collective impact on force steadiness (FS). Twelve young adult males performed knee extension tasks under three different tibial rotation conditions: internal rotation (IR), neutral rotation (NR), and external rotation (ER). All tasks involved submaximal isometric contractions at the knee joint. Participants performed submaximal isometric contractions at three knee joint angles (30 degrees, 60 degrees, and 90 degrees) and three target force levels (10%, 40%, 70% of maximum voluntary isometric contraction (MVIC)). The electromyographic (EMG) signals from vastus medialis (VM), vastus lateralis (VL), and rectus femoris (RF) were recorded. FS was quantified using standard deviation (SD) and coefficient of variation (CV) of force output, while EMG steadiness was assessed using SD and CV of the EMG signals. Muscle morphology features, including muscle thickness (MT) and echo intensity (EI), were assessed via ultrasound imaging. Submaximal isometric contractions revealed significant FS differences across target force levels and knee angles (angle x target force interaction: F(4,44) = 3.073, P = 0.026). With increasing target force from 10 to 70% MVIC, quadriceps EMG signals showed progressive amplitude increases in all three muscles (RF, VM and VL, p 0.05), it significantly influenced EMG characteristics at 60 degrees knee angle (tibial rotation x target force x muscle interaction: F(8,88) = 3.357, P = 0.026). Strong correlations were found between force steadiness and EMG steadiness (r = 0.557-0.657, P < 0.001). Additionally, RF echo intensity positively correlated with EMG_SD in both ER and NR positions (r = 0.644 and 0.619 respectively, P < 0.05), while quadriceps muscle thickness negatively correlated with EMG_CV in ER position (r = -0.600, P < 0.05). FS revealed that absolute fluctuations (SD) increased with target force levels, while relative fluctuations (CV) decreased. Target force levels and knee joint angles significantly influence FS, whereas tibial rotation does not directly affect FS during isometric contractions. However, tibial rotation interestingly affects EMG steadiness, and the variability in EMG-derived muscle activation correlates with both FS and muscle morphology parameters. These findings provide valuable insights into neuromuscular control mechanisms during force production. | Yuan, Han; Kim, Maeng-Kyu | Kyungpook Natl Univ, Grad Sch, Dept Phys Educ, Daegu, South Korea; Kyungpook Natl Univ, Sports Sci Res Inst, Daegu, South Korea | 58784951600; 26021932400 | 2021327546@knu.ac.kr; | SCIENTIFIC REPORTS | SCI REP-UK | 2045-2322 | 15 | 1 | SCIE | MULTIDISCIPLINARY SCIENCES | 2024 | 3.9 | 18.1 | 0 | 2025-05-07 | 0 | 0 | Force steadiness; Ultrasound; Electromyography; Neuromuscular control; Tibial rotation | MOTOR-UNIT SYNCHRONIZATION; QUADRICEPS FEMORIS MUSCLE; ACTIVATION PATTERNS; YOUNG; PERFORMANCE; TORQUE | Electromyography; Force steadiness; Neuromuscular control; Tibial rotation; Ultrasound | Adult; Biomechanical Phenomena; Electromyography; Humans; Isometric Contraction; Knee Joint; Male; Muscle, Skeletal; Quadriceps Muscle; Range of Motion, Articular; Rotation; Tibia; Young Adult; adult; biomechanics; electromyography; human; knee joint; male; muscle isometric contraction; physiology; quadriceps femoris muscle; range of motion; rotation; skeletal muscle; tibia; young adult | English | 2025 | 2025-04-21 | 10.1038/s41598-025-93163-6 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |||
○ | ○ | Article | Nonmass lesions on preoperative MRI in breast cancer patients: clinical implications and prognostic significance | This study aimed to investigate the clinical importance and prognostic value of nonmass lesions (NMLs) identified via preoperative magnetic resonance imaging (MRI) in patients with breast cancer, with an emphasis on understanding how these lesions affect treatment decisions and survival outcomes. A retrospective analysis was conducted on 6971 patients diagnosed with breast cancer who underwent surgery at Asan Medical Center, Seoul, between January 2000 and December 2021. Patients were categorized based on the presence or absence of NMLs on preoperative MRI. Various clinicopathological parameters were compared, and survival outcomes, such as overall survival (OS), distant metastasis-free survival (DMFS), regional recurrence-free survival (RFS), and local recurrence-free survival (LFS), were analyzed using Kaplan-Meier and Cox regression methods. Subgroup analyses were performed based on the type of surgery and the administration of neoadjuvant chemotherapy and adjuvant radiation therapy. Of the total cohort, 21.9% (n = 1524) had NMLs. The presence of NMLs was associated with a significant improvement in OS (P = 0.017) for the entire patient group. Multivariate analysis revealed the presence of NMLs as a favorable prognostic factor (hazard ratio 0.47, 95% confidence intervals 0.25-0.90, P = 0.022). Subgroup analyses demonstrated significantly improved OS, DMFS, and RFS outcomes for patients with NMLs who underwent mastectomy after neoadjuvant chemotherapy. NMLs on preoperative MRI in patients with breast cancer are associated with improved overall survival (OS) and serve as an independent prognostic factor. However, further research is needed to elucidate the underlying reasons for these outcomes. | Moon, Joon Suk; Yoo, Tae Kyung; Kim, Jisun; Chung, Il Yong; Ko, Beom Seok; Kim, Hee Jeong; Lee, Jong Won; Son, Byung Ho; Lee, Sae Byul | Univ Ulsan, Asan Med Ctr, Coll Med, Div Breast Surg,Dept Surg, 88 Olymp Ro 43 Gil, Seoul 05505, South Korea; Kyungpook Natl Univ, Chilgok Hosp, Sch Med, Dept Surg, Daegu, South Korea | 55172800300; 59907829700; 55913243600; 59707757100; 59707524000; 57205135299; 59676912300; 59677470000; 59676348000 | newstar153@hanmail.net; | SCIENTIFIC REPORTS | SCI REP-UK | 2045-2322 | 15 | 1 | SCIE | MULTIDISCIPLINARY SCIENCES | 2024 | 3.9 | 18.1 | 0 | 2025-06-11 | 0 | 0 | Breast cancer; MRI; Nonmass lesion; Mastectomy; Neoadjuvant chemotherapy; Radiation therapy | SURGICAL ADJUVANT BREAST; POSTMASTECTOMY RADIOTHERAPY; POSTOPERATIVE RADIOTHERAPY; LOCOREGIONAL RECURRENCE; RADIATION-THERAPY; NON-MASS; ENHANCEMENT; WOMEN; CHEMOTHERAPY; MASTECTOMY | Breast cancer; Mastectomy; MRI; Neoadjuvant chemotherapy; Nonmass lesion; Radiation therapy | Adult; Aged; Breast Neoplasms; Female; Humans; Kaplan-Meier Estimate; Magnetic Resonance Imaging; Mastectomy; Middle Aged; Neoadjuvant Therapy; Preoperative Care; Prognosis; Retrospective Studies; adult; aged; breast tumor; diagnostic imaging; female; human; Kaplan Meier method; mastectomy; middle aged; mortality; neoadjuvant therapy; nuclear magnetic resonance imaging; pathology; preoperative care; procedures; prognosis; retrospective study; surgery; therapy | English | 2025 | 2025-05-23 | 10.1038/s41598-025-03025-4 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |||
○ | ○ | Article | Proton response and neutron spectrum unfolding by solution-grown trans-stilbene scintillator | This paper reports the proton response function for solution-grown trans-stilbene scintillator from 1 to 25 MeVee and its application for unfolding neutron spectra of Cf-252 and AmBe sources. Low energy proton response was measured by the Time-of-Flight technique at the Korea Research Institute of Standards and Science. In contrast, high energy response was investigated using a 45 MeV proton beam at the Korea Institute of Radiological & Medical Sciences. The photomultiplier tube nonlinearity effect was studied and corrected for high-energy measurement. Several energy calibration functions were examined to minimize the effects of the light yield nonlinearity of the scintillator, which was observed as nonnegligible in the measurement. A new approach was applied to obtain 1.3 ns full width at half maximum timing resolution in the Time-of-Flight experiment when using a 500 Mega samples per second analog to digital converter. The unfolding was performed using the iterative Bayesian method without any constraints, smoothness, or assumption of prior knowledge of original spectra. For the first time, we achieved one of the best unfolding results for both Cf-252 and AmBe spectra. Results from this study are promising for fast neutron spectroscopy. | Quang, Nguyen Duy; Kim, H. J.; Kim, Sunghwan; Kang, Sinchul; Vuong, Phan Quoc; Hieu, Phan Bao Quoc; Cuong, Nguyen Kien; Yoon, Y. S.; Nam, Uk-Won | Kyungpook Natl Univ, Dept Phys, Daegu 41566, South Korea; Dalat Nucl Res Inst, Da Lat, Vietnam; Cheongju Univ, Dept Radiol Sci, Cheongju 28503, South Korea; Korea Res Inst Stand & Sci, Daejeon 34113, South Korea; Korea Astron & Space Sci Inst, Space Sci Div, Daejeon 34055, South Korea | 57223004024; 58483690400; 36165569900; 57191418531; 57207618553; 58775861600; 59549314300; 35243798200; 6602840978 | hongjoo@knu.ac.kr; | SCIENTIFIC REPORTS | SCI REP-UK | 2045-2322 | 15 | 1 | SCIE | MULTIDISCIPLINARY SCIENCES | 2024 | 3.9 | 18.1 | 0 | 2025-05-07 | 0 | 0 | Trans-stilbene scintillator; Proton response; Nonproportionality; Neutron spectrum unfolding; Time-of-Flight technique | DETECTOR | Neutron spectrum unfolding; Nonproportionality; Proton response; Time-of-Flight technique; Trans-stilbene scintillator | californium 252; proton; stilbene; adult; article; Bayes theorem; calibration; controlled study; fast neutron radiation; female; major clinical study; male; neutron radiation; nonlinear system; proton radiation; scintillation | English | 2025 | 2025-01-28 | 10.1038/s41598-024-85087-4 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |||
○ | ○ | Article | Python algorithm package for automated Estimation of major legume root traits using two dimensional images | A simple Python algorithm was used to estimate the four major root traits: total root length (TRL), surface area (SA), average diameter (AD), and root volume (RV) of legumes (adzuki bean, mung bean, cowpea, and soybean) based on two-dimensional images. Four different thresholding methods; Otsu, Gaussian adaptive, mean adaptive and triangle threshold were used to know the effect of thresholding in root trait estimation and to optimize the accuracy of root trait estimation. The results generated by the algorithm applied to 400 legume root images were compared with those generated by two separate software (WinRHIZO and RhizoVision), and the algorithm was validated using ground truth data. Distance transform method was used for estimating SA, AD, and RV and ConnectedComponentsWithStat function for TRL estimation. Among the thresholding methods, Otsu thresholding worked well for distance transform, while triangle threshold was effective for TRL. All the traits showed a high correlation with an R-2 >= 0.98 (p < 0.001) with the ground truth data. The root mean square error (RMSE) and mean bias error (MBE) were also minimal when comparing the algorithm-derived values to the ground truth values, with RMSE and MBE both < 10 for TRL, < 6 for SA, and < 0.5 for AD and RV. This lower value of error metrics indicates smaller differences between the algorithm-derived values and software-derived values. Although the observed error metrics were minimal for both software, the algorithm-derived root traits were closely aligned with those derived from WinRHIZO. We provided a simple Python algorithm for easy estimation of legume root traits where the images can be analyzed without any incurring expenses, and being open source; it can be modified by an expert based on their requirements. | Ghimire, Amit; Chung, Yong Suk; Jeong, Sungmoon; Kim, Yoonha | Kyungpook Natl Univ, Dept Appl Biosci, Daegu 41566, South Korea; Kyungpook Natl Univ, Dept Integrat Biol, Daegu 41566, South Korea; Jeju Natl Univ, Dept Plant Resources & Environm, Jeju 63243, South Korea; Kyungpook Natl Univ Hosp, Biomed Res Inst, Res Ctr AI Med, Daegu 41940, South Korea; Kyungpook Natl Univ, Sch Med, Dept Med Informat, Daegu 41566, South Korea; Kyungpook Natl Univ, Upland Field Machinery Res Ctr, Daegu 41566, South Korea | ghimire, amit/KCK-1842-2024; Chung, Yong/V-6909-2019 | 57823220200; 36983850100; 23100090400; 57224866763 | kyh1229@knu.ac.kr; | SCIENTIFIC REPORTS | SCI REP-UK | 2045-2322 | 15 | 1 | SCIE | MULTIDISCIPLINARY SCIENCES | 2024 | 3.9 | 18.1 | 4.56 | 2025-05-07 | 1 | 1 | Image processing; Legumes; Python algorithm; Root traits; Threshold | SOFTWARE; YIELD | Image processing; Legumes; Python algorithm; Root traits; Threshold | Algorithms; Fabaceae; Image Processing, Computer-Assisted; Plant Roots; Software; adzuki bean; algorithm; article; benchmarking; cowpea; diagnosis; Fabaceae; human; image processing; legume; mung bean; plant root; root length; root mean squared error; soybean; surface area; procedures; software | English | 2025 | 2025-03-01 | 10.1038/s41598-025-91993-y | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||
○ | ○ | Article | Rapid detection of soybean nutrient deficiencies with YOLOv8s for precision agriculture advancement | Early detection of nutrient deficiencies is crucial for optimizing crop yields and ensuring sustainable agricultural practices. This study presents a novel application of the YOLOv8s object detection model for identifying nitrogen, phosphorus, and potassium deficiencies in soybean plants. Employing a unique dataset from a long-term nutrient-deficient field maintained for over 40 years, we trained and evaluated the model on 6,020 red, green, and blue images of soybean leaves exhibiting nutrient stress conditions. The YOLOv8s model achieved exceptional performance, with a mean average precision (mAP@0.5) of 99.18% during training and 98.51% for validation. Precision rates for individual nutrient deficiencies ranged from 90.03 to 96.54%, with highly accurate potassium deficiency detection. The model demonstrated robust generalization across diverse field conditions, processing images in 3.46 ms each, making it suitable for real-time applications. This research significantly advances the field of precision agriculture by providing a fast, accurate, and scalable method for detecting early nutrient deficiency in soybean crops, potentially revolutionizing fertilizer management practices and contributing to more sustainable farming systems. | Jeong, Minsoo; Park, Sihyun; Kwon, Sook-Min; Lim, Kyeongmo; Jung, Da-Ryung; Lee, Hong-Seok; Kim, Hei Jung; Shin, Jae-Ho | Kyungpook Natl Univ, Dept Appl Biosci, Daegu 41566, South Korea; Kyungpook Natl Univ, Dept Integrat Biotechnol, Daegu 41566, South Korea; RDA, NICS, Dept Southern Area Crop Sci, Miryang 50424, South Korea; Kyungpook Natl Univ, NGS Core Facil, Daegu 41566, South Korea | 57292954900; 58642859800; 59469456700; 57805008000; 57221116102; 57194274429; 59469280600; 57224125922 | jhshin@knu.ac.kr; | SCIENTIFIC REPORTS | SCI REP-UK | 2045-2322 | 15 | 1 | SCIE | MULTIDISCIPLINARY SCIENCES | 2024 | 3.9 | 18.1 | 0 | 2025-05-07 | 0 | 0 | Artificial intelligence; Nutrient deficiency; Soybeans; Smart farming; YOLOv8 | Artificial intelligence; Nutrient deficiency; Smart farming; Soybeans; YOLOv8 | Agriculture; Crops, Agricultural; Fertilizers; Glycine max; Nitrogen; Nutrients; Phosphorus; Plant Leaves; Potassium; fertilizer; nitrogen; phosphorus; potassium; agriculture; chemistry; crop; growth, development and aging; metabolism; nutrient; plant leaf; procedures; soybean | English | 2025 | 2025-04-21 | 10.1038/s41598-024-83295-6 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||||
○ | ○ | Article | SmartBerry for AI-based growth stage classification and precision nutrition management in strawberry cultivation | Agriculture is vital for human sustenance and economic stability, with increasing global food demand necessitating innovative practices. Traditional farming methods have caused significant environmental damage, highlighting the need for sustainable practices like nutrition management. This paper addresses the emerging integration of artificial intelligence (AI) in agriculture, focusing on the specific challenge of growth stage classification of strawberry plants for optimized nutrition management. While AI has been successfully applied in various agricultural domains, such as plant stress detection and growth monitoring, the precise classification of strawberry growth stages remains underexplored. Accurate growth stage identification is vital for timely nutrient application, directly impacting yield and fruit quality. Our research identifies common gaps in existing literature, including limited or inaccessible datasets, outdated methodologies, and insufficient benchmarking. To overcome these shortcomings, we introduce a robust greenhouse-based dataset covering seven distinct strawberry growth stages, captured under diverse conditions. We then benchmark multiple state-of-the-art models on this dataset, finding that EfficientNetB7 achieves a testing accuracy of 0.837-demonstrating the promise of AI-driven approaches for precise and sustainable nutrient management in horticulture. | Darlan, Daison; Ajani, Oladayo S.; An, Joon Woo; Bae, Nan Yeon; Lee, Bram; Park, Tusan; Mallipeddi, Rammohan | Kyungpook Natl Univ, Dept Artificial Intelligence, Daegu, South Korea; UBN Corp, Daegu, South Korea; Kyungpook Natl Univ, Dept Smart Bioind Mech Engn, Daegu, South Korea; Kyungpook Natl Univ, Smart Agr Innovat Ctr, Daegu, South Korea; Kyungpook Natl Univ, Dept Appl Biosci, Daegu, South Korea | AJANI, Oladayo/HIR-9607-2022; Park, Tusan/C-2057-2012; Mallipeddi, Rammohan/AAL-5306-2020; Darlan, Daison/KQA-9542-2024 | 58164208500; 57465126000; 59749765900; 59749109500; 59749109600; 57202780408; 25639919900 | tusan.park@knu.ac.kr; mallipeddi.ram@gmail.com; | SCIENTIFIC REPORTS | SCI REP-UK | 2045-2322 | 15 | 1 | SCIE | MULTIDISCIPLINARY SCIENCES | 2024 | 3.9 | 18.1 | 0 | 2025-05-07 | 0 | 0 | Strawberry; Nutrition management; Growth stage classification; Smart farming | Growth stage classification; Nutrition management; Smart farming; Strawberry | Agriculture; Artificial Intelligence; Fragaria; Fruit; agriculture; artificial intelligence; classification; Fragaria; fruit; growth, development and aging; procedures | English | 2025 | 2025-04-23 | 10.1038/s41598-025-97168-z | 바로가기 | 바로가기 | 바로가기 | 바로가기 |
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