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○ ○ Article ⓘ Evaluation of morphological, physiological, and biochemical traits for assessing drought resistance in eleven tree species ⓘ The frequency and severity of drought are expected to increase due to climate change; therefore, selection of tree species for afforestation should consider drought resistance of the species for maximum survival and conservation of natural habitats. In this study, three soil moisture regimes: control (100% precipitation), mild drought (40% reduction in precipitation), and severe drought (80% reduction in precipitation) were applied to six gymnosperm and five angiosperm species for two consecutive years. We quantified the drought resistance index based on the root collar diameter and assessed the correlation between species drought resistance and other morphological, physiological, and biochemical traits by regression analysis. The prolonged drought stress altered the morphological, physiological, and biochemical traits, but the responses were species-specific. The species with high drought resistance had high leaf mass per area (LMA), photosynthetic rate (Pn), and midday leaf water potential (psi MD), and low carbon isotopic discrimination (513C), flavonoid and polyphenol content, superoxide dismutase and DPPH radical scavenging activity. The highly drought-resistant species had a relatively less decrease in leaf size, Pn, and predawn leaf water potential (psi PD), and less increase in 513C, abscisic acid and sucrose content, and LMA compared to the control. The interannual variation in drought resistance (delta Rd) was positively correlated with the species hydroscopic slope (isohydric and anisohydric). Korean pine was highly resistant, sawtooth oak, hinoki cypress, East Asian white birch, East Asian ash, and mono maple were highly susceptible, and Korean red pine, Japanese larch, Sargent cherry, needle fir, and black pine were moderate in drought resistance under long-term drought. These findings will help species selection for afforestation programs and es-tablishment of sustainable forests, especially of drought-tolerant species, under increased frequency and inten-sity of spring and summer droughts. (c) 2021 Elsevier B.V. All rights reserved. ⓘ Bhusal, Narayan; Lee, Minsu; Lee, Hojin; Adhikari, Arjun; Han, Ah Reum; Han, Areum; Kim, Hyun Seok ⓘ Seoul Natl Univ, Dept Agr Forestry & Bioresources, Seoul 08826, South Korea; Seoul Natl Univ, Res Inst Agr & Life Sci, Seoul 08826, South Korea; Seoul Natl Univ, Interdisciplinary Program Agr & Forest Meteorol, Seoul 08826, South Korea; Kyungpook Natl Univ, Sch Appl Biosci, Daegu 41566, South Korea; Natl Inst Ecol, Div Basic Res, Seocheon Gun 33657, South Korea; Natl Ctr Agro Meteorol, Seoul 08826, South Korea ⓘ Adhikari, Arjun/JCO-3306-2023; Adhikari, Arjun/AAV-6297-2021; Lee, Hojin/IXN-8380-2023; Bhusal, Narayan/AAE-9086-2019 ⓘ 57199327486; 57197791550; 57222255185; 57195601415; 36843269500; 57216158934; 57203630265 ⓘ cameroncrazies@snu.ac.kr; ⓘ SCIENCE OF THE TOTAL ENVIRONMENT ⓘ SCI TOTAL ENVIRON ⓘ 0048-9697 1879-1026 779 SCIE ENVIRONMENTAL SCIENCES ⓘ 2021 10.754 9.1 5.63 2025-07-30 ⓘ 144 144 Anisohydric; isohydric behavior; Antioxidant; Drought resistance; Endogenous hormone; Leaf morphology; Photosynthetic response; Plantation species ⓘ CLIMATE-CHANGE IMPACTS; NORWAY SPRUCE; PHOTOSYNTHETIC RESPONSE; HYDRAULIC CONDUCTANCE; STRESS RESPONSES; ABSCISIC-ACID; LEAF SIZE; GROWTH; WATER; MORTALITY ⓘ Anisohydric/isohydric behavior; Antioxidant; Drought resistance; Endogenous hormone; Leaf morphology; Photosynthetic response; Plantation species ⓘ Droughts; Forests; Plant Leaves; Quercus; Trees; Water; Betula platyphylla; Cupressus; Gymnospermae; Magnoliophyta; Pinus koraiensis; Pinus nigra; Pinus resinosa; Pseudolarix kaempferi; Quercus acutissima; Antioxidants; Climate change; Conservation; Physiological models; Physiology; Plants (botany); Reforestation; Regression analysis; Soil moisture; 1,1 diphenyl 2 picrylhydrazyl; abscisic acid; amino acid; flavonoid; jasmonic acid; polyphenol; proline; sucrose; superoxide dismutase; water; Anisohydric/isohydric behavior; Biochemical traits; Drought resistance; Endogenous hormones; Leaf morphology; Morphological traits; Photosynthetic response; Physiological trait; Plantation species; Tree species; afforestation; angiosperm; antioxidant; biochemistry; drought resistance; gymnosperm; hormone; leaf morphology; photosynthesis; physiological response; plantation; soil moisture; Abies holophylla; Acer pictum; afforestation; amino acid analysis; angiosperm; Article; Betula platyphylla; carbohydrate analysis; carbon isotopic discrimination; Chamaecyparis obtusa; climate change; controlled study; DPPH radical scavenging assay; drought resistance; environmental protection; Fraxinus rhynchophylla; gymnosperm; Larix kaempferi; leaf mass per area; leaf water potential; nonhuman; photosynthetic rate; phytochemistry; Pinus densiflora; Pinus koraiensis; Pinus thunbergii; plant growth; plant leaf; plant leaf size; plant parameters; plant physiology; plant structures; precipitation; priority journal; Prunus sargentii; Quercus acutissima; root collar diameter; soil moisture; tree; drought; forest; oak; tree; Drought ⓘ English 2021 2021-07-20 10.1016/j.scitotenv.2021.146466 ⓘ 바로가기 바로가기 바로가기 바로가기
○ ○ Article ⓘ Hispidulin alleviates 2,4-dinitrochlorobenzene and house dust mite extract-induced atopic dermatitis-like skin inflammation ⓘ Atopic dermatitis (AD) is a chronic inflammatory skin disorder that affects 10?20% of the world?s population. Therefore, the discovery of drugs for the treatment of AD is important for human health. Hispidulin (HPD; also known as scutellarein 6-methyl ether or dinatin) is a natural flavone that exerts anti-inflammatory effects. In the present study, the effectiveness of HPD on AD-like skin inflammation was investigated. We used a mouse AD model through repeated exposure of mice to 2,4-dinitrochlorobenzene and house dust mite extract (Dermato-phagoides farinae extract, DFE) to the ears. In addition, tumor necrosis factor-? and interferon-?-activated ker-atinocytes (HaCaT cells) were used to investigate the underlying mechanism of HPD action. Oral administration of HPD alleviated AD-like skin inflammations: it reduced ear thickness; serum immunoglobulin (Ig)E, DFE-specific IgE, and IgG2a levels; and inflammatory cell infiltration. HPD reduced the expression of pro-inflammatory cytokines and chemokines through inhibition of signal transducer and activator of transcription 1 nuclear factor-?B in HaCaT cells. Taken together, these results suggest that HPD could be a potential drug candidate for the treatment of AD. ⓘ Kang, Jinjoo; Lee, Soyoung; Kim, Namkyung; Dhakal, Hima; Choi, Young-Ae; Kwon, Taeg Kyu; Khang, Dongwoo; Kim, Sang-Hyun ⓘ Kyungpook Natl Univ, Cell & Matrix Res Inst, Sch Med, Dept Pharmacol, Daegu, South Korea; Korea Res Inst Biosci & Biotechnol, Immunoregulatory Mat Res Ctr, Jeongeup, South Korea; Keimyung Univ, Sch Med, Dept Immunol, Daegu, South Korea; Gachon Univ, Sch Med, Dept Physiol, Incheon, South Korea ⓘ ⓘ 57216977823; 8537269200; 57216981866; 57195999763; 7404777420; 7202206057; 26039177500; 57210450420 ⓘ dkhang@gachon.ac.kr;shkim72@knu.ac.kr; ⓘ BIOMEDICINE & PHARMACOTHERAPY ⓘ BIOMED PHARMACOTHER ⓘ 0753-3322 1950-6007 137 SCIE MEDICINE, RESEARCH & EXPERIMENTAL;PHARMACOLOGY & PHARMACY ⓘ 2021 7.419 9.1 0.93 2025-07-30 ⓘ 11 11 Atopic dermatitis; Hispidulin; House dust mite; Keratinocytes ⓘ CELLS; ACTIVATION; EOSINOPHILS; RECRUITMENT; MACROPHAGES; CYTOKINES; DELIVERY; EOTAXIN; ALLERGY; PHASE ⓘ Atopic dermatitis; Hispidulin; House dust mite; Keratinocytes ⓘ Animals; Anti-Allergic Agents; Antigens, Dermatophagoides; Dermatitis, Atopic; Dinitrochlorobenzene; Eosinophils; Female; Flavones; Immunoglobulins; Keratinocytes; Mast Cells; Mice; Mice, Inbred BALB C; Pyroglyphidae; Skin; 1 chloro 2,4 dinitrobenzene; dexamethasone; gamma interferon; hispidulin; house dust mite allergen extract; immunoglobulin E; immunoglobulin enhancer binding protein; immunoglobulin G2a; STAT1 protein; tumor necrosis factor; 1 chloro 2,4 dinitrobenzene; antiallergic agent; flavone derivative; hispidulin; house dust allergen; immunoglobulin; animal cell; animal experiment; animal model; animal tissue; antiinflammatory activity; Article; atopic dermatitis; cell activation; cell infiltration; controlled study; Dermatophagoides; drug efficacy; ear thickness; eosinophil; female; HaCat cell line; immunoglobulin blood level; inflammatory cell; keratinocyte; mast cell; mouse; nonhuman; priority journal; animal; atopic dermatitis; Bagg albino mouse; drug effect; immunology; metabolism; pathology; Pyroglyphidae; skin ⓘ English 2021 2021-05 10.1016/j.biopha.2021.111359 ⓘ 바로가기 바로가기 바로가기 바로가기
○ ○ Article ⓘ MXene-coated biochar as potential biocathode for improved microbial electrosynthesis system ⓘ Microbial electrosynthesis (MES) holds tremendous large scale energy storage potential. By promoting the bio-conversion of carbon dioxide (bicarbonate) into useful chemical commodities, this technique utilizes renewable energy and reduces carbon footprint. However, expensive electrode materials, low current densities, and multiple electrosynthesis products are major challenges to this field. To this end, this study examines a multilayered and conductive MXene structure that was coated on a cost-effective biochar substrate and tested as a MES cathode. These results show this coating yielded improved electrical conductivity, increased charge transfer efficiency, and selective microbial enrichment characteristics, resulting in a 2.3-fold increase in cathodic current production in comparison to the uncoated biochar. Moreover, an increase in active sites improved mass transfer and microbial growth, producing 1.7-fold increase in butyrate in comparison to the uncoated control. Considering that electrode attached microbial communities play a major role in final products, microbial community analyses was completed, suggesting that selective microbial enrichment was promoted as Firmicutes (66%), Proteobacteria (13%), and Bacteroidetes (12%) (i.e., exoelectrogenic and butyrate producing phyla) which were dominant in the MXene-coated biochar biofilm. These results show that biochar modification is an effective technique for achieving selective products through MES. (C) 2021 Elsevier B.V. All rights reserved. ⓘ Tahir, Khurram; Miran, Waheed; Jang, Jiseon; Maile, Nagesh; Shahzad, Asif; Moztahida, Mokrema; Ghani, Ahsan Abdul; Kim, Bolam; Jeon, Hyeji; Lee, Dae Sung ⓘ Kyungpook Natl Univ, Dept Environm Engn, Buk Gu, 80 Daehak Ro, Daegu 41566, South Korea; Natl Inst Mat Sci, Int Ctr Mat Nanoarchitecton, 1-1 Namiki, Tsukuba, Ibaraki 3050044, Japan; Korea Radioact Waste Agcy, R&D Inst Radioact Wastes, 174 Gajeong Ro, Daejeon 34129, South Korea ⓘ Tahir, Khurram/KFS-5321-2024; Miran, Waheed/G-6671-2016; Maile, Nagesh/AFD-8838-2022 ⓘ 57207114377; 57195984440; 56611137400; 57193804539; 57190382338; 57202904382; 57218294089; 57208922438; 57218291200; 55568524907 ⓘ daesung@knu.ac.kr; ⓘ SCIENCE OF THE TOTAL ENVIRONMENT ⓘ SCI TOTAL ENVIRON ⓘ 0048-9697 1879-1026 773 SCIE ENVIRONMENTAL SCIENCES ⓘ 2021 10.754 9.1 1.29 2025-07-30 ⓘ 27 32 Biochar; MXene; Biocathode; Microbial electrosynthesis; Butyrate ⓘ CARBON ELECTRODE; REDUCTION; TEMPERATURE; NANOSHEETS; HYDROGEN; CATHODE; IMPACT ⓘ Biocathode; Biochar; Butyrate; Microbial electrosynthesis; MXene ⓘ Carbon Dioxide; Charcoal; Electrodes; Microbiota; Bacteroidetes; Firmicutes; Proteobacteria; Carbon dioxide; Carbon footprint; Cathodes; Charge transfer; Cost effectiveness; Energy storage; Mass transfer; Volatile fatty acids; biochar; butyric acid; charcoal; ribosome RNA; unclassified drug; biochar; carbon dioxide; Charge transfer efficiency; Electrical conductivity; Electrode material; Enrichment characteristics; Low current density; Microbial communities; Microbial community analysis; Renewable energies; biochar; biofilm; electrical conductivity; electrode; electrokinesis; mass transfer; microbial community; Article; Bacteroidetes; biofilm; chronoamperometry; controlled study; cost effectiveness analysis; cyclic voltammetry; electric conductivity; Firmicutes; Fourier transform infrared spectroscopy; high performance liquid chromatography; impedance spectroscopy; microbial community; microbial electrosynthesis; microbial growth; nonhuman; polymerase chain reaction; Proteobacteria; scanning electron microscopy; synthesis; X ray photoemission spectroscopy; electrode; microflora; Microorganisms ⓘ English 2021 2021-06-15 10.1016/j.scitotenv.2021.145677 ⓘ 바로가기 바로가기 바로가기 바로가기
○ ○ Article ⓘ Phytotoxicological effects of engineered nanoparticles: An emerging nanotoxicology ⓘ Recent innovations in the field of nanoscience and technology and its proficiency as a part of inter-disciplinary science has set an eclectic display in innumerable branches of science, a majority in aliened health science of human and agriculture. Modern agricultural practices have been shifting towards the implementation of nanotechnology-based solutions to combat various emerging problems ranging from safe delivery of nutrients to sustainable approaches for plant protection. In these processes, engineered nanoparticles (ENPs) are widely used as nanocarriers (to deliver nutrients and pesticides) due to their high permeability, efficacy, biocompatibility, and biodegradability properties. Even though the constructive nature of nanoparticles (NPs), nanomaterials (NMs), and other modified or ENPs towards sustainable development in agriculture is referenced, the darker side i.e., eco-toxicological effects is still not covered to a larger extent. The overwhelming usage of these trending NMs has led to continuous persistence in the ecosystem, and their interface with the biotic and abiotic community, degradation lanes and intervention, which might lead to certain beneficial or malefic effects. Metal oxide NPs and polymeric NPs (Alginate, chitosan, and polyethylene glycol) are the most used ENPs, which are posing the nature of beneficial as well as environmentally concerning hazardous materials depending upon their fate and persistence in the ecosystem. The cautious usage of NMs in a scientific way is most essential to harness beneficial aspects of NMs in the field of agriculture whilst minimizing the eco-toxicological effects. The current review is fo-cused on the toxicological effects of various NMs on plant physiology and health. It details interactions of plant in-tracellular components between applied/persistent NMs, which have brought out drastic changes in seed germination, crop productivity, direct and indirect interaction at the enzymatic as well as nuclear levels. In conclu-sion, ENPs can pose as genotoxicants that may alter the plant phenotype if not administered appropriately. (c) 2021 Elsevier B.V. All rights reserved. ⓘ Jogaiah, Sudisha; Paidi, Murali Krishna; Venugopal, Krishnan; Geetha, Nagaraja; Mujtaba, Muhammad; Udikeri, Shashikant Shiddappa; Govarthanan, Muthusamy ⓘ Karnatak Univ, PG Dept Biotechnol & Microbiol, Lab Plant Healthcare & Diagnost, Dharwad 580003, Karnataka, India; Cent Salt & Marine Chem Res Inst, CSIR, AcSIR, Gijubhai Badheka Marg, Bhavnagar 364002, Gujarat, India; Vivekanandha Coll Arts & Sci Women, Dept Biochem, Tiruchengode 637205, Tamil Nadu, India; Univ Mysore, Nanobiotechnol Lab, Dept Studies Biotechnol, Mysuru 570006, Karnataka, India; Aalto Univ, Sch Chem Engn, Dept Bioprod & Biosyst, FI-00076 Espoo, Finland; Univ Agr Sci, Dharwad Farm, Agr Res Stn, Dharwad 580005, Karnataka, India; Kyungpook Natl Univ, Dept Environm Engn, 80 Daehak Ro, Daegu 41566, South Korea; Ankara Univ, Inst Biotechnol, TR-06110 Ankara, Turkey ⓘ Muthusamy, Govarthanan/C-1491-2014; Govarthanan, Muthusamy/C-1491-2014; Jogaiah, Sudisha/ABE-8334-2021; Krishna, Paidi/AAJ-4689-2020; Mujtaba, Muhammad/U-6130-2018 ⓘ 16744010100; 57194271137; 58692890100; 57194453109; 56704961200; 9632925500; 54881927600 ⓘ jsudish@kud.ac.in;gova.muthu@gmail.com; ⓘ SCIENCE OF THE TOTAL ENVIRONMENT ⓘ SCI TOTAL ENVIRON ⓘ 0048-9697 1879-1026 801 SCIE ENVIRONMENTAL SCIENCES ⓘ 2021 10.754 9.1 0.84 2025-07-30 ⓘ 53 69 Agriculture; Metallic oxides; Engineered nanoparticles; Polymeric nanoparticles; Phytotoxicity ⓘ METAL-OXIDE NANOPARTICLES; WALLED CARBON NANOTUBES; TITANIUM-DIOXIDE NANOPARTICLES; SILVER NANOPARTICLES; IN-VITRO; CERIUM OXIDE; PHOTOCATALYTIC ACTIVITY; CHITOSAN NANOPARTICLES; ARABIDOPSIS-THALIANA; LA2O3 NANOPARTICLES ⓘ Agriculture; Engineered nanoparticles; Metallic oxides; Phytotoxicity; Polymeric nanoparticles ⓘ Agriculture; Ecosystem; Humans; Metal Nanoparticles; Nanostructures; Nanotechnology; Biocompatibility; Biodegradability; Biohazards; Ecosystems; Nanoparticles; Nanoscience; Oxides; Seed; carotenoid; chitosan; glutathione; glutathione peroxidase; glutathione reductase; graphene oxide; hydrogel; lignin; malonaldehyde; metal oxide; nanocomposite; parathion methyl; phytohormone; polymer nanoparticle; reactive oxygen metabolite; silver nanoparticle; titanium dioxide; metal nanoparticle; nanomaterial; Agricultural practices; Engineered nanoparticles; Health science; Metallic oxides; Nano science and technologies; Nanotoxicology; Phytotoxicity; Plant protection; Polymeric nanoparticles; Toxicological effects; ecotoxicology; environmental fate; genotoxicity; nanoparticle; permeability; phenotype; physiological response; phytotoxicity; polymer; sustainable development; abiotic stress; aquatic environment; biodegradability; biotransformation; cell ultrastructure; chlorophyll content; comet assay; controlled study; cytotoxicity; DNA damage; DNA replication; drug metabolism; dry weight; enzyme activity; gene expression; gene overexpression; genotoxicity; human; LC50; nanotechnology; nanotoxicology; Nicotiana tabacum; nonhuman; oxidative stress; particle size; photosynthesis; phytotoxicity; plant growth; plant height; plant physiology; polymerization; random amplified polymorphic DNA; Review; root growth; root length; scanning electron microscopy; seedling; signal transduction; sustainable development; tissue engineering; toxicity testing; toxicology; upregulation; agriculture; ecosystem; Metals ⓘ English 2021 2021-12-20 10.1016/j.scitotenv.2021.149809 ⓘ 바로가기 바로가기 바로가기 바로가기
○ ○ Article ⓘ Protective effects of p-coumaric acid against high-fat diet-induced metabolic dysregulation in mice ⓘ p-Coumaric acid (PC), a naturally occurring phytochemical, possesses antioxidant and anti-inflammatory properties; however, the mechanisms underlying its protective effects against obesity-related metabolic dysfunction are largely unknown. Here, we treated C57BL/6J mice to a high-fat diet (HFD) with or without PC (10 mg/kg body weight/day) for 16 weeks to determine whether PC ameliorates HFD-induced obesity, insulin resistance, inflammation, and non-alcoholic fatty liver disease (NAFLD). We found no significant differences in food intake and body weight between the groups. However, PC-treated mice showed significantly lower white adipose tissue (WAT) weight, adipocyte size, and plasma leptin level, which were associated with decreased lipogenic enzyme activity and mRNA expression of their genes in the epididymal WAT. Moreover, hepatic lipogenic enzymes activities and expression of their genes and proteins were decreased with concomitant increases in hepatic fatty acid oxidation and mRNA expression of its gene; fecal lipid excretion was significantly increased, resulting in decreased liver weight, hepatic lipid levels, lipid droplet accumulation, and plasma aspartate aminotransferase and lipid levels. Additionally, PC-treated mice showed lower fasting blood glucose, plasma resistin, and MCP-1 levels, HOMA-IR, and mRNA expression of inflammatory genes in the epididymal WAT and liver. Our findings reveal potential mechanisms underlying the action of PC against HFD-induced adiposity, NAFLD, and other metabolic disturbances. ⓘ Yoon, Dae Seong; Cho, Su Yeon; Yoon, Hye Jin; Kim, Sang Ryong; Jung, Un Ju ⓘ Pukyong Natl Univ, Dept Food Sci & Nutr, 45 Yongso Ro, Busan 48513, South Korea; Kyungpook Natl Univ, Sch Life Sci, BK21 Plus KNU Creat BioRes Grp, Daegu, South Korea; Kyungpook Natl Univ, Brain Sci & Engn Inst, Daegu, South Korea ⓘ ⓘ 57226359863; 57226369497; 57226351589; 56486163800; 7007119425 ⓘ jungunju@naver.com; ⓘ BIOMEDICINE & PHARMACOTHERAPY ⓘ BIOMED PHARMACOTHER ⓘ 0753-3322 1950-6007 142 SCIE MEDICINE, RESEARCH & EXPERIMENTAL;PHARMACOLOGY & PHARMACY ⓘ 2021 7.419 9.1 2.31 2025-07-30 ⓘ 31 30 p-Coumaric acid; Obesity; Insulin resistance; Inflammation; Non-alcoholic fatty liver disease; High-fat diet ⓘ STEAROYL-COA DESATURASE-1; INDUCED HEPATIC STEATOSIS; DE-NOVO LIPOGENESIS; ADIPOSE-TISSUE; INSULIN-RESISTANCE; PHOSPHATIDATE PHOSPHOHYDROLASE; SPECTROPHOTOMETRIC ASSAY; GENE-EXPRESSION; RISK-FACTORS; IN-VITRO ⓘ High-fat diet; Inflammation; Insulin resistance; Non-alcoholic fatty liver disease; Obesity; p-Coumaric acid ⓘ Adiposity; Animals; Anti-Inflammatory Agents; Antioxidants; Blood Glucose; Coumaric Acids; Diet, High-Fat; Inflammation; Insulin Resistance; Male; Mice; Mice, Inbred C57BL; Non-alcoholic Fatty Liver Disease; Obesity; Resistin; aspartate aminotransferase; cholesterol; fat droplet; feces lipid; glucose; immunoglobulin enhancer binding protein; insulin; interleukin 6; leptin; lipid; messenger RNA; monocyte chemotactic protein 1; para coumaric acid; resistin; toll like receptor 2; toll like receptor 4; triacylglycerol; tumor necrosis factor; antiinflammatory agent; antioxidant; coumaric acid; p-coumaric acid; resistin; adipocyte; adipose tissue; animal experiment; animal model; animal tissue; Article; aspartate aminotransferase blood level; body weight; cell size; controlled study; diet supplementation; down regulation; dyslipidemia; enzyme activity; enzyme inhibition; epididymal tissue; fatty acid oxidation; food intake; gene expression; glucose blood level; homeostasis model assessment; hypolipidemic activity; inflammation; insulin blood level; insulin resistance; lipid blood level; lipid diet; lipid liver level; lipid metabolism; lipid storage; lipogenesis; liver protection; liver weight; male; morphology; mouse; nonalcoholic fatty liver; nonhuman; obesity; obesity management; protein blood level; protein expression level; protein synthesis inhibition; total cholesterol level; triacylglycerol blood level; weight; white adipose tissue; adverse event; animal; blood; C57BL mouse; drug effect; inflammation; insulin resistance; lipid diet; nonalcoholic fatty liver; obesity ⓘ English 2021 2021-10 10.1016/j.biopha.2021.111969 ⓘ 바로가기 바로가기 바로가기 바로가기
○ Article ⓘ Wastewater treatment and fouling control in an electro algae-activated sludge membrane bioreactor ⓘ The effect of addition of algae to activated sludge as active biomass in membrane bioreactors (MBRs) and electro-MBRs (e-MBRs) for wastewater remediation was examined in this study. The performances of Algae-Activated Sludge Membrane Bioreactor (AAS-MBR) and electro Algae-Activated Sludge Membrane Bioreactor (e-AAS-MBR) were compared to those observed in conventional MBR and e-MBR, which were previously reported and utilized activated sludge as biomass. The effect of application of electric field was also examined by the comparison of performances of e-AAS-MBR and AAS-MBR. Similar chemical oxygen demand (COD) reduction efficiencies of AAS-MBR, e-AAS-MBR, MBR, and e-MBR (98.35 ± 0.35%, 99.12 ± 0.08%, 97.70 ± 1.10%, and 98.10 ± 1.70%, respectively) were observed. The effect of the algae-activated sludge system was significantly higher in the nutrient removals. Ammoniacal nitrogen (NH3−N) removal efficiencies of AAS-MBR and e-AAS-MBR were higher by 43.89% and 26.61% than in the conventional MBR and e-MBR, respectively. Phosphate phosphorous (PO43−-P) removals were also higher in AAS-MBR and e-AAS-MBR by 6.43% and 2.66% than those in conventional MBR and e-MBR. Membrane fouling rates in AAS-MBR and e-AAS-MBR were lower by 57.30% and 61.95% than in MBR and e-MBR, respectively. Lower concentrations of fouling substances were also observed in the reactors containing algae-activated sludge biomass. Results revealed that addition of algae improved nutrient removal and membrane fouling mitigation. The study also highlighted that the application of electric field in the e-AAS-MBR enhanced organic contaminants and nutrients removal, and fouling rate reduction. © 2021 Elsevier B.V. ⓘ Corpuz, Mary Vermi Aizza; Borea, Laura; Senatore, Vincenzo; Castrogiovanni, Fabiano; Buonerba, Antonio; Oliva, Giuseppina; Ballesteros, Florencio; Zarra, Tiziano; Belgiorno, Vincenzo; Choo, Kwang-Ho; Hasan, Shadi W.; Naddeo, Vincenzo ⓘ Environmental Engineering Program, National Graduate School of Engineering, University of the Philippines, Diliman, 1101, Quezon City, Philippines; Sanitary Environmental Engineering Division (SEED), Department of Civil Engineering, University of Salerno, Fisciano, 84084, Italy; Sanitary Environmental Engineering Division (SEED), Department of Civil Engineering, University of Salerno, Fisciano, 84084, Italy; Sanitary Environmental Engineering Division (SEED), Department of Civil Engineering, University of Salerno, Fisciano, 84084, Italy; Sanitary Environmental Engineering Division (SEED), Department of Civil Engineering, University of Salerno, Fisciano, 84084, Italy, Inter-University Centre for Prediction and Prevention of Relevant Hazards (Centro Universitario per la Previsione e Prevenzione Grandi Rischi, C.U.G.RI.), Via Giovanni Paolo II, Fisciano, SA, Italy; Sanitary Environmental Engineering Division (SEED), Department of Civil Engineering, University of Salerno, Fisciano, 84084, Italy; Jr, Environmental Engineering Program, National Graduate School of Engineering, University of the Philippines, Diliman, 1101, Quezon City, Philippines, Department of Chemical Engineering, College of Engineering, University of the Philippines, Diliman, 1101, Quezon City, Philippines; Sanitary Environmental Engineering Division (SEED), Department of Civil Engineering, University of Salerno, Fisciano, 84084, Italy; Sanitary Environmental Engineering Division (SEED), Department of Civil Engineering, University of Salerno, Fisciano, 84084, Italy; Department of Environmental Engineering, Kyungpook National University (KNU), 80 Daehak-ro, Bukgu, 41566, Daegu, South Korea; Center for Membranes and Advanced Water Technology (CMAT), Department of Chemical Engineering, Khalifa University of Science and Technology, Abu Dhabi, United Arab Emirates; Sanitary Environmental Engineering Division (SEED), Department of Civil Engineering, University of Salerno, Fisciano, 84084, Italy ⓘ ⓘ 57218353260; 56442610000; 57219694936; 57221015267; 36670901300; 20735093300; 36664314600; 16176861400; 6508019638; 7102083272; 35738779100; 57225215311 ⓘ vnaddeo@unisa.it; ⓘ Science of the Total Environment ⓘ SCI TOTAL ENVIRON ⓘ 0048-9697 1879-1026 786 SCIE ENVIRONMENTAL SCIENCES ⓘ 2021 10.754 9.1 2.77 2025-07-30 ⓘ 67 ⓘ ⓘ Activated sludge; Algae; Electrochemical; Fouling; Membrane bioreactor; Wastewater ⓘ Bioreactors; Membranes, Artificial; Sewage; Waste Water; Water Purification; Activated sludge process; Algae; Bioconversion; Biomass; Chemical oxygen demand; Coagulation; Efficiency; Electric fields; Membrane fouling; Membranes; Microfiltration; Nitrates; Nitrogen removal; Nutrients; Reduction; Surface waters; Wastewater; Wastewater treatment; ammonia; ammoniacal nitrogen; nitrogen; phosphate; phosphoric phosphorus; phosphorus; unclassified drug; Activated sludge; Active biomass; Conventional membrane bioreactors; Effect of addition; Electro-membranes; Electrochemicals; Fouling control; Nutrient removal; Sludge membrane bioreactor; Wastewater remediation; activated sludge; alga; bioreactor; chemical oxygen demand; electric field; electrochemistry; fouling; membrane; pollutant removal; wastewater treatment; activated sludge; Article; biomass; chemical oxygen demand; Chlorella vulgaris; coculture; electric field; electrochemical oxidation; electrochemistry; electrocoagulation; fouling control; microalga; microbial consortium; municipal wastewater; nonhuman; nutrient removal; oxidation; priority journal; waste water management; artificial membrane; bioreactor; sewage; wastewater; water management; Bioreactors ⓘ English Final 2021 10.1016/j.scitotenv.2021.147475 ⓘ 바로가기 바로가기 바로가기
○ ○ Article ⓘ Inflow characteristics for generating axis-switching phenomenon of heated rectangular jets ⓘ Flow characteristics due to the axis-switching phenomenon are numerically investigated for heated rectangular jets with typical inlet conditions and three aspect ratios (AR), namely 1.0, 1.5, and 2.0. To examine various inlets and their relation to the axis-switching flow, an inflow generator is newly devised, wherein jet flows from an orifice plate and a long pipe nozzle can be realized. Depending on the inlet conditions, the 45. and 90 degrees axis-switching phenomena for the rectangular jet are observed to appear differently. As the aspect ratio increases and jet temperature decreases, the axis-switching positions are delayed downstream. It is observed that the strong and weak states of the axis-switching flows can be interpreted by the crossover locations of the jet half-widths and the saddle-shaped velocity profile is not the only main factor for axis-switching flow. As the jet temperature increases, a well-mixed state is quickly obtained, and the mixing performance of the square jet is better than that of the rectangular jet. For both isothermal and heated jets, the flow mixing is improved by the axis-switching flow. Various analyses are attempted to find the specific inlet structure for the axis-switching flow. From the results, the special features for inflow criterion producing axis-switching flow can be explained by factors such as the deformation parameter, interrelations between shear strain and normal rates, turbulence-driven and geometry-driven streamwise vortices, and normal Reynolds stresses. ⓘ Kim, Won Hyun; Park, Tae Seon ⓘ Kyungpook Natl Univ, Sch Mech Engn, 80 Daehak Ro, Daegu 41566, South Korea ⓘ ⓘ 55360608000; 7401801892 ⓘ tsparkjp@knu.ac.kr; ⓘ COMPUTERS & MATHEMATICS WITH APPLICATIONS ⓘ COMPUT MATH APPL ⓘ 0898-1221 1873-7668 86 SCIE MATHEMATICS, APPLIED ⓘ 2021 3.218 9.2 0.29 2025-07-30 ⓘ 4 4 Heated rectangular jet; Inflow condition; Axis switching; Aspect ratio; Geometry-driven vortices; Mixing enhancement ⓘ ⓘ Aspect ratio; Axis switching; Geometry-driven vortices; Heated rectangular jet; Inflow condition; Mixing enhancement ⓘ Aspect ratio; Intake systems; Mixing; Reynolds number; Shear flow; Shear strain; Strain rate; Deformation parameter; Flow charac-teristics; Inlet conditions; Jet temperature; Mixing performance; Reynolds stress; Streamwise vortices; Velocity profiles; Switching ⓘ English 2021 2021-03-15 10.1016/j.camwa.2021.02.001 ⓘ 바로가기 바로가기 바로가기 바로가기
○ ○ Article ⓘ Alteration of the Soil Microbiota in Ginseng Rusty Roots: Application of Machine Learning Algorithm to Explore Potential Biomarkers for Diagnostic and Predictive Analytics ⓘ Conceptualization to utilize microbial composition as a prediction tool has been widely applied in human cohorts, yet the potential capacity of soil microbiota as a diagnostic tool to predict plant phenotype remains unknown. Here, we collected 130 soil samples which are 54 healthy controls and 76 ginseng rusty roots (GRRs). Alpha diversities including Shannon, Simpson, Chaol, and phylogenetic diversity were significantly decreased in GRR (P < 0.05). Moreover, we identified 30 potential biomarkers. The optimized markers were obtained through fivefold cross-validation on a support vector machine and yielded a robust area under the curve of 0.856. Notably, evaluation of multi-index classification performance including accuracy, F1-score, and Kappa coefficient also showed robust discriminative capability (90.99%, 0.903, and 0.808). Taken together, our results suggest that the disease affects the microbial community and offers the potential ability of soil microbiota to identifying farms at the risk of GRR. ⓘ Kang, Gi-Ung; Ibal, Jerald Conrad; Lee, Seungjun; Jang, Myeong Hwan; Park, Yeong-Jun; Kim, Min-Chul; Park, Tae-Hyung; Kim, Min-Sueng; Kim, Ryeong-Hui; Shin, Jae-Ho ⓘ Kyungpook Natl Univ, Dept Appl Biosci, Daegu 41566, South Korea; Pukyong Natl Univ, Dept Food Sci & Nutr, Busan 48513, South Korea; Punggi Ginseng Res Inst GBARES, Youngju 36023, South Korea; Kyungpook Natl Univ, Dept Intergrat Biotechnol, Daegu 41566, South Korea ⓘ ; Lee, Seungjun/S-9826-2019; Ibal, Jerald/JYQ-0493-2024 ⓘ 57211635810; 57196117664; 56437333700; 57195627689; 56275800900; 57203466596; 57224855198; 57224861638; 57222756419; 57224125922 ⓘ jhshin@knu.ac.kr; ⓘ JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY ⓘ J AGR FOOD CHEM ⓘ 0021-8561 1520-5118 69 29 SCIE CHEMISTRY, APPLIED;FOOD SCIENCE & TECHNOLOGY;AGRICULTURE, MULTIDISCIPLINARY ⓘ 2021 5.895 9.3 0.44 2025-07-30 ⓘ 6 6 soil microbiota; machine learning; ginseng; SVM; Panax ginseng ⓘ PLANT-GROWTH; PANAX-GINSENG; BACILLUS-SUBTILIS; AMERICAN GINSENG; GUT MICROBIOME; BACTERIUM; ROT ⓘ ginseng; machine learning; Panax ginseng; soil microbiota; SVM ⓘ Biomarkers; Humans; Machine Learning; Microbiota; Panax; Phylogeny; Plant Roots; Soil; Biomarkers; Curve fitting; Diagnosis; Learning algorithms; Soils; Support vector machines; biological marker; Area under the curves; Classification performance; Kappa coefficient; Microbial communities; Microbial composition; Phylogenetic diversity; Potential ability; Potential capacity; human; machine learning; microflora; Panax; phylogeny; plant root; soil; Predictive analytics ⓘ English 2021 2021-07-28 10.1021/acs.jafc.1c01314 ⓘ 바로가기 바로가기 바로가기 바로가기
○ ○ Article ⓘ Anti-osteoclastogenic Effects of Indole Alkaloids Isolated from Barley (Hordeum vulgare Var. Hexastichon) Grass ⓘ As part of our continuous program to identify new potential candidates for controlling osteolytic bone diseases from natural products, the alkaloid fraction of barley (Hordeum vulgare var. hexastichon) grass (HVA) significantly inhibited RANKL-induced osteoclast formation and protected mice from LPS-induced bone loss. A phytochemical investigation of HVA afforded nine indole alkaloids, including one new compound [hordeumin A (1)] and eight known analogues (2-9). Of them, four (1, 2, 4, and 5) were anti-osteoclastogenic compounds. Of these four, compound 5 significantly suppressed RANKL-induced osteoclast formation, actin ring formation, and bone resorption in a concentration-dependent manner. It also suppressed the RANKL-induced NF-kappa B and MAPK signaling pathways and the activation of c-Fos and NFATc1. Compound 5 also reduced the expression levels of osteoclastspecific marker genes, including TRAP, CtsK, DC-STAMP, OSCAR, and MMP9. Our findings suggest that HVA and its alkaloid constituents could be valuable candidates for the prevention and treatment of osteolytic bone diseases. ⓘ Manh Tuan Ha; Phuong Thao Tran; Huynh Nguyen Khanh Tran; Kim, Okwha; Kim, Jeong Ah; Lee, Jeong-Hyung; Min, Byung Sun ⓘ Daegu Catholic Univ, Coll Pharm, Drug Res & Dev Ctr, Gyeongbuk 38430, South Korea; Kangwon Natl Univ, Coll Nat Sci, Dept Biochem, Chunchon 24341, South Korea; Kyungpook Natl Univ, Coll Pharm, Res Inst Pharmaceut Sci, Daegu 41566, South Korea ⓘ Tuan, Ha/AAW-5444-2021 ⓘ 57189040434; 56701990600; 57194387860; 56599865200; 24722570900; 26662839900; 55663638200 ⓘ jhlee36@kangwon.ac.kr;bsmin@cu.ac.kr; ⓘ JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY ⓘ J AGR FOOD CHEM ⓘ 0021-8561 1520-5118 69 44 SCIE CHEMISTRY, APPLIED;FOOD SCIENCE & TECHNOLOGY;AGRICULTURE, MULTIDISCIPLINARY ⓘ 2021 5.895 9.3 0.53 2025-07-30 ⓘ 6 6 Hordeum vulgare var. hexastichon; barley grass; indole alkaloids; osteoclastogenesis; RANKL; NFATc1 ⓘ NF-KAPPA-B; BONE; INDUCTION; DIFFERENTIATION; DISEASES; HEALTH; DIM; I3C; L. ⓘ barley grass; Hordeum vulgare var. hexastichon; indole alkaloids; NFATc1; osteoclastogenesis; RANKL ⓘ Animals; Bone Resorption; Cell Differentiation; Hordeum; Indole Alkaloids; Mice; NF-kappa B; NFATC Transcription Factors; Osteoclasts; Osteogenesis; Poaceae; RANK Ligand; Bone; Mammals; Polycyclic aromatic hydrocarbons; Proteins; Value engineering; immunoglobulin enhancer binding protein; indole alkaloid; osteoclast differentiation factor; transcription factor NFAT; Barley grass; Bone disease; Hexasticha; Hordeum vulgare; Hordeum vulgare var.; Indole alkaloids; NFATc1; Osteoclast formation; Osteoclastogenesis; RANKL; animal; bone development; cell differentiation; drug therapy; genetics; Hordeum; mouse; osteoclast; osteolysis; Poaceae; Alkaloids ⓘ English 2021 2021-11-10 10.1021/acs.jafc.1c04337 ⓘ 바로가기 바로가기 바로가기 바로가기
○ ○ Article ⓘ Crystal Structure and Functional Characterization of the Bifunctional N-(5′-Phosphoribosyl)anthranilate Isomerase-indole-3-glycerol-phosphate Synthase from Corynebacterium glutamicum ⓘ L-Tryptophan is known as an aromatic amino acid and one of the essential amino acids that must be ingested through various additives or food. TrpCF is a bifunctional enzyme that has indole-glycerol-phosphate synthase (IGPS) and phosphoribosylanthranilate isomerase (PRAI) activity. In this report, we identified the crystal structure of TrpCF from Corynebacterium glutamicum (CgTrpCF) and successfully elucidated the active site by attaching rCdRP similar to the substrate and product of the TrpCF reaction. Also, we revealed that CgTrpCF shows a conformational change at the loops upon substrate binding. We analyzed amino acid sequences of the homologues of CgTrpCF, and the residues of the substrate-binding site in TrpCF were highly conserved except for some residues. These less conserved residues were replaced by site-directed mutagenesis experiments. Consequently, we obtained the CgTrpCF(P294K) (PRAI(CD/P294K)) variant that has enhanced activity. ⓘ Park, Woojin; Son, Hyeoncheol Francis; Lee, Donghoon; Kim, Il-Kwon; Kim, Kyung-Jin ⓘ Kyungpook Natl Univ, Sch Life Sci, KNU Inst Microorganisms, Four KNU Creat BioRes Grp BK21, Daegu 41566, South Korea ⓘ Kim, Kyung-Jin/MVY-3405-2025 ⓘ 57211680374; 56015598000; 57207418964; 56547774100; 55510867400 ⓘ kkim@knu.ac.kr; ⓘ JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY ⓘ J AGR FOOD CHEM ⓘ 0021-8561 1520-5118 69 42 SCIE CHEMISTRY, APPLIED;FOOD SCIENCE & TECHNOLOGY;AGRICULTURE, MULTIDISCIPLINARY ⓘ 2021 5.895 9.3 0.18 2025-07-30 ⓘ 2 3 N-(5'-phosphoribosyl)anthranilate isomerase; indole-3-glycerol-phosphate synthase; bifunctional enzyme; Corynebacterium glutamicum; L-tryptophan biosynthesis ⓘ PHOSPHORIBOSYL ANTHRANILATE ISOMERASE; INDOLEGLYCEROL-PHOSPHATE SYNTHASE; L-TRYPTOPHAN; BIOTECHNOLOGICAL PRODUCTION; SULFOLOBUS-SOLFATARICUS; ESCHERICHIA-COLI; SUBSTRATE-ANALOG; ENZYME; BIOSYNTHESIS ⓘ bifunctional enzyme; Corynebacterium glutamicum; indole-3-glycerol-phosphate synthase; L -tryptophan biosynthesis; N-(5′-phosphoribosyl)anthranilate isomerase ⓘ Aldose-Ketose Isomerases; Corynebacterium glutamicum; Indole-3-Glycerol-Phosphate Synthase; Isomerases; Additives; Amino acids; Biochemistry; Enzymes; Glycerol; Polycyclic aromatic hydrocarbons; indole 3 glycerol phosphate synthase; isomerase; phosphoribosylanthranilate isomerase; Anthranilate; Bifunctional enzymes; Corynebacterium glutamicum; Crystals structures; Indole-3-glycerol phosphate synthase; Isomerases; L -tryptophan biosynthesis; L-tryptophan; N-(5′-phosphoribosyl)anthranilate isomerase; Tryptophan biosynthesis; Corynebacterium glutamicum; genetics; Crystal structure ⓘ English 2021 2021-10-27 10.1021/acs.jafc.1c05132 ⓘ 바로가기 바로가기 바로가기 바로가기
○ ○ Article ⓘ Crystal Structure and Regiospecificity of Catechol O-Methyltransferase from Niastella koreensis ⓘ Catechol O-methyltransferase (COMT) is an enzyme that transfers a methyl group to the catechol-derivative substrates using S-adenosyl-l-methionine (SAM) and Mg2+. We report the biochemical and structural analysis of COMT from Niastella koreensis (NkCOMT). NkCOMT showed the highest activity with Mg2+, although the enzyme also showed a significant level of activity with Cu2+ and Zn2+. NkCOMT structures complexed with SAH and Mg2+ elucidated how the enzyme stabilized the cosubstrate and the metal ion and revealed that the region near the SAM binding site undergoes conformational changes upon the binding of the cosubstrate and the metal ion. We also identified the catechol binding pocket of the enzyme and explained a broad substrate specificity of the bacterial enzyme and its ability to accommodate the catechol derivatives. In addition, we developed the NkCOMT(E211R) and NkCOMT(E211K) variants that showed both enhanced activities and regiospecificity for the production of the para-forms. Our study provides a structural basis for regiospecificity of NkCOMT, which is related with the conformational change upon binding of SAM and Mg2+. ⓘ Lee, Seul Hoo; Kim, Bongsang; Kim, Kyung-Jin ⓘ Kyungpook Natl Univ, Sch Life Sci, KNU Creat BioRes Grp, Daegu 41566, South Korea; Kyungpook Natl Univ, KNU Inst Microorganisms, Daegu 41566, South Korea ⓘ Kim, Kyung-Jin/MVY-3405-2025 ⓘ 57205261937; 57221311592; 55510867400 ⓘ kkim@knu.ac.kr; ⓘ JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY ⓘ J AGR FOOD CHEM ⓘ 0021-8561 1520-5118 69 8 SCIE CHEMISTRY, APPLIED;FOOD SCIENCE & TECHNOLOGY;AGRICULTURE, MULTIDISCIPLINARY ⓘ 2021 5.895 9.3 0.7 2025-07-30 ⓘ 8 8 catechol O-methyltransferase; Niastella koreensis; S-adenosyl-l-methionine; regiospecificity ⓘ BINDING-AFFINITY; BIOSYNTHESIS; METHYLATION; INHIBITOR; STABILITY; GENE ⓘ catechol O-methyltransferase; Niastella koreensis; regiospecificity; S-adenosyl-l-methionine ⓘ Bacteroidetes; Catechol O-Methyltransferase; S-Adenosylmethionine; Substrate Specificity; Amino acids; Binding sites; Enzymes; Metal ions; Metals; Phenols; Substrates; ademetionine; catechol methyltransferase; Bacterial enzymes; Catechol derivatives; Catechol-O-methyltransferase; Conformational change; Regiospecificity; S adenosyl l methionines; Structural basis; Substrate specificity; Bacteroidetes; enzyme specificity; genetics; metabolism; Crystal structure ⓘ English 2021 2021-03-03 10.1021/acs.jafc.0c07621 ⓘ 바로가기 바로가기 바로가기 바로가기
○ ○ Article ⓘ Dual α-1,4-and β-1,4-Glycosidase Activities by the Novel Carbohydrate-Binding Module in α-L-Fucosidase from Vibrio sp. Strain EJY3 ⓘ Carbohydrates are structurally and functionally diverse materials including polysaccharides, and marine organisms are known to have many enzymes for the breakdown of complex polysaccharides. Here, we identified an alpha-L-fucosidase enzyme from the marine bacterium Vibrio sp. strain EJY3 (VejFCD) that has dual alpha-1,4-glucosidic and beta-1,4-galactosidic specificities. We determined the crystal structure of VejFCD and provided the structural basis underlying the dual alpha- and beta-glycosidase activities of the enzyme. Unlike other three-domain FCDs, in VejFCD, carbohydrate-binding module-B (CBM-B) with a novel beta-sandwich fold tightly contacts with the CatD/CBM-B main body and provides key residues for the beta-1,4-glycosidase activity of the enzyme. The phylogenetic tree analysis suggests that only a few FCDs from marine microorganisms have the key structural features for dual alpha-1,4-and beta-1,4-glycosidase activities. This study provides the structural insights into the mechanism underlying the novel glycoside hydrolase activities and could be applied for more efficient utilization in the hydrolysis of complex carbohydrates in biotechnological applications. ⓘ Hong, Hwaseok; Kim, Do Hyoung; Seo, Hogyun; Kim, Kyoung Heon; Kim, Kyung-Jin ⓘ Kyungpook Natl Univ, Sch Life Sci, KNU Creat BioRes Grp, Daegu 41566, South Korea; Kyungpook Natl Univ, KNU Inst Microbiol, Daegu 41566, South Korea; Korea Univ, Grad Sch, Dept Biotechnol, Seoul 02841, South Korea; Pohang Univ Sci & Technol, Pohang Accelerator Lab, Pohang 37673, South Korea ⓘ Kim, Kyung-Jin/MVY-3405-2025; Kim, Jinkwon/AAR-6729-2021; Kim, Kyoung/F-1059-2013; Kim, Kyoung Heon/F-1059-2013 ⓘ 57208592877; 56739384900; 57189697998; 34770896300; 55510867400 ⓘ khekim@korea.ac.kr;kkim@knu.ac.kr; ⓘ JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY ⓘ J AGR FOOD CHEM ⓘ 0021-8561 1520-5118 69 11 SCIE CHEMISTRY, APPLIED;FOOD SCIENCE & TECHNOLOGY;AGRICULTURE, MULTIDISCIPLINARY ⓘ 2021 5.895 9.3 0.61 2025-07-30 ⓘ 10 9 alpha-L-fucosidase; Vibrio sp. strain EJY3; dual alpha-1,4-and beta-1,4-glycosidase: carbohydrate-binding module; marine microorganism ⓘ SULFATED POLYSACCHARIDES; PURIFICATION; LIVER; IMMUNOGLOBULIN; MEMBRANE; MODEL; TOOLS ⓘ dual α-1,4- and β-1,4-glycosidase: carbohydrate-binding module; marine microorganism; Vibrio sp. strain EJY3; α- l -fucosidase ⓘ alpha-L-Fucosidase; Carbohydrates; Glycoside Hydrolases; Hydrolysis; Phylogeny; Substrate Specificity; Vibrio; Bacteria; Enzyme activity; Marine biology; Polysaccharides; alpha levo fucosidase; carbohydrate; glycosidase; Biotechnological applications; Carbohydrate binding modules; Complex carbohydrates; Glycoside hydrolases; Marine microorganism; Phylogenetic tree analysis; Structural feature; Structural insights; enzyme specificity; genetics; hydrolysis; metabolism; phylogeny; Vibrio; Crystal structure ⓘ English 2021 2021-03-24 10.1021/acs.jafc.0c08199 ⓘ 바로가기 바로가기 바로가기 바로가기
○ ○ Article ⓘ Identification of a Potential Gene for Elevating ω-3 Concentration and Its Efficiency for Improving the ω-6/ω-3 Ratio in Soybean ⓘ This present study was to identify a novel candidate gene that contributes to the elevated a-linolenic acid (ALA, omega-3) concentration in PE2166 from mutagenesis of Pungsannamul. Major loci qALA5₁ and qALA5₂ were detected on chromosome 5 of soybean through quantitative trait loci mapping analyses of recombinant inbred lines. With next-generation sequencing of parental lines and Pungsannamul and recombinant analyses, a potential gene, Glyma.05g221500 (HD), controlling elevated ALA concentration was identified. HD is a homeodomain-like transcriptional regulator that may regulate the expression level of microsomal omega-3 fatty acid desaturase (FAD3) genes responsible for the conversion of linoleic acid into ALA in the fatty acid biosynthetic pathway. In addition, we hypothesized that a combination of mutant alleles, HD, and either of microsomal delta-12 fatty acid desaturase 2-1 (FAD2-1) could reduce the omega-6/omega-3 ratio. In populations where HD, FAD2-1A, and FAD2-1B genes were segregated, a combination of a hd allele from PE2166 and either of the variant FAD2-1 alleles was sufficient to reduce the omega-6/omega-3 ratio in seeds. ⓘ Jo, Hyun; Kim, Minsu; Cho, Hyeontae; Ha, Bo-Keun; Kang, Sungtaeg; Song, Jong Tae; Lee, Jeong-Dong ⓘ Kyungpook Natl Univ, Dept Appl Biosci, Daegu 41566, South Korea; Chonnam Natl Univ, Dept Appl Plant Sci, Gwangju 61186, South Korea; Dankook Univ, Dept Crop Sci & Biotechnol, Cheonan 16890, South Korea ⓘ ⓘ 57194539358; 58635134600; 57222763635; 7006749575; 9736708500; 57797134000; 40462075100 ⓘ jdlee@knu.ac.kr; ⓘ JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY ⓘ J AGR FOOD CHEM ⓘ 0021-8561 1520-5118 69 13 SCIE CHEMISTRY, APPLIED;FOOD SCIENCE & TECHNOLOGY;AGRICULTURE, MULTIDISCIPLINARY ⓘ 2021 5.895 9.3 0.7 2025-07-30 ⓘ 7 8 soybean; omega-3; omega-6; FAD3; homeodomain transcription factor ⓘ ALPHA-LINOLENIC ACID; KNOWLEDGE-BASE SOYKB; SEED OIL CONTENT; FATTY-ACID; CARDIOVASCULAR-DISEASE; PRODUCE SOYBEANS; READ ALIGNMENT; WEB RESOURCE; WILD; OVEREXPRESSION ⓘ FAD3; homeodomain transcription factor; soybean; ω-3; ω-6 ⓘ Alleles; Fatty Acid Desaturases; Plant Proteins; Quantitative Trait Loci; Seeds; Soybeans; Linoleic acid; Plants (botany); acyl coenzyme A desaturase; plant protein; Alpha linolenic acids; Biosynthetic pathway; Next-generation sequencing; Omega-3-fatty acids; Omega-6/omega-3 ratios; Quantitative trait loci mappings; Recombinant inbred lines; Transcriptional regulator; allele; genetics; plant seed; quantitative trait locus; soybean; Genes ⓘ English 2021 2021-04-07 10.1021/acs.jafc.0c05830 ⓘ 바로가기 바로가기 바로가기 바로가기
○ ○ Review ⓘ Interactions between Brassica Biofumigants and Soil Microbiota: Causes and Impacts ⓘ Biofumigation is used to control soil-borne plant diseases, and it has paramount importance to reduce the cost of chemical fumigants. Information about the field control efficacies and impacts of Brassica-based biofumigation (BBF) on soil bacterial and fungal microbiota is scattered in the literature. Therefore, this review summarizes and discusses the nature and the underlying causes of soil bacterial and fungal community dynamics in response to BBF. In addition, the major factors influencing the interaction between a biofumigant and soil microbiota are discussed. The pros and cons of BBF to soil microbiota and the subsequent impacts on sustainable farming practices are also highlighted. ⓘ Tagele, Setu Bazie; Kim, Ryeong-Hui; Shin, Jae-Ho ⓘ Kyungpook Natl Univ, Dept Appl Biosci, Daegu 41566, South Korea; Kyungpook Natl Univ, Dept Integrat Biol, Daegu 41566, South Korea ⓘ Tagele, Setu Bazie/IYJ-1959-2023; shin, Jaeho/K-6792-2013 ⓘ 57202007103; 57222756419; 57224125922 ⓘ jhshin@knu.ac.kr; ⓘ JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY ⓘ J AGR FOOD CHEM ⓘ 0021-8561 1520-5118 69 39 SCIE CHEMISTRY, APPLIED;FOOD SCIENCE & TECHNOLOGY;AGRICULTURE, MULTIDISCIPLINARY ⓘ 2021 5.895 9.3 0.5 2025-07-30 ⓘ 21 20 biofumigation; Brassica; glucosinolates; isothiocyanates; microbiota ⓘ PHYTOPHTHORA-NICOTIANAE BREDA; SEED-MEAL; COMMUNITY STRUCTURE; ROOT-ROT; HYDROLYSIS PRODUCTS; ALLYL ISOTHIOCYANATE; CHEMICAL FUMIGATION; FUNGAL COMMUNITIES; MYCORRHIZAL FUNGI; MYCELIAL GROWTH ⓘ biofumigation; Brassica; glucosinolates; isothiocyanates; microbiota ⓘ Brassica; Fumigation; Microbiota; Soil; Soil Microbiology; Disease control; Fumigation; Biofumigant; Biofumigation; Brassica; Field-control; Glucosinolates; Isothiocyanates; Microbiotas; Plant disease; Soil microbiota; Underlying cause; Brassica; fumigation; microbiology; microflora; soil; Soils ⓘ English 2021 2021-10-06 10.1021/acs.jafc.1c03776 ⓘ 바로가기 바로가기 바로가기 바로가기
○ ○ Article ⓘ Melittin-loaded Iron Oxide Nanoparticles Prevent Intracranial Arterial Dolichoectasia Development through Inhibition of Macrophage-mediated Inflammation ⓘ Rationale: In intracranial arterial dolichoectasia (IADE) development, the feedback loop between inflammatory cytokines and macrophages involves TNF-alpha and NF-KB signaling pathways and leads to subsequent MMP-9 activation and extracellular matrix (ECM) degeneration. In this proof-of-concept study, melittin-loaded L-arginine-coated iron oxide nanoparticle (MeLioN) was proposed as the protective measure of IADE formation for this macrophage-mediated inflammation and ECM degeneration. Methods: IADE was created in 8-week-old C57BL/6J male mice by inducing hypertension and elastase injection into a basal cistern. Melittin was loaded on the surface of ION as a core-shell structure (hydrodynamic size, 202.4 nm; polydispersity index, 0.158). Treatment of MeLioN (2.5 mg/kg, five doses) started after the IADE induction, and the brain was harvested in the third week. In the healthy control, disease control, and MeLioN-treated group, the morphologic changes of the cerebral arterial wall were measured by diameter, thickness, and ECM composition. The expression level of MMP-9, CD68, MCP-1, TNF-alpha, and NF-kappa B was assessed from immunohistochemistry, polymerase chain reaction, and Western blot assay. Results: MeLioN prevented morphologic changes of cerebral arterial wall related to IADE formation by restoring ECM alterations and suppressing MMP-9 expression. MeLioN inhibited MCP-1 expression and reduced CD68-positive macrophage recruitments into cerebral arterial walls. MeLioN blocked TNF-alpha activation and NF-kappa B signaling pathway. In the Sylvian cistern, co-localization was found between the CD68-positive macrophage infiltrations and the MeLioN distributions detected on Prussian Blue and T2* gradient-echo MRI, suggesting the role of macrophage harboring MeLioN. Conclusions: The macrophage infiltration into the arterial wall plays a critical role in the MMP-9 secretion. MeLioN, designed for ION-mediated melittin delivery, effectively prevents IADE formation by suppressing macrophage-mediated inflammations and MMP activity. MeLioN can be a promising strategy preventing IADE development in high-risk populations. ⓘ Vu, Huy Duc; Huynh, Phuong Tu; Ryu, Junghwa; Kang, Ung Rae; Youn, Sung Won; Kim, Hongtae; Ahn, Hyun Jin; Park, Kwankyu; Hwang, Soon-Kyung; Chang, Young-Chae; Lee, Yong Jig; Lee, Hui Joong; Lee, Jongmin ⓘ Daegu Catholic Univ, Dept Radiol, Sch Med, 3056-6,Daemyung 4 Dong, Daegu 705718, South Korea; Daegu Catholic Univ, Dept Anat, Sch Med, Daegu, South Korea; Daegu Catholic Univ, Dept Pathol, Sch Med, Daegu, South Korea; Daegu Catholic Univ, Dept Mol Biol, Sch Med, Daegu, South Korea; Daegu Catholic Univ, Dept Plast Surg, Sch Med, Daegu, South Korea; Kyungpook Natl Univ, Dept Radiol, Sch Med, Daegu, South Korea ⓘ Youn, Sung-Won/AAH-1723-2020; LEE, JIN/Q-3108-2018; Lee, Joonseok/AFQ-8078-2022 ⓘ 57280380200; 56829091800; 57682112500; 49863442100; 57206373939; 57926222300; 57280132600; 56984258600; 12545069900; 7501843107; 55245743100; 23485776600; 55689919700 ⓘ ysw10adest@cu.ac.kr; ⓘ INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES ⓘ INT J BIOL SCI ⓘ 1449-2288 17 14 SCIE BIOCHEMISTRY & MOLECULAR BIOLOGY ⓘ 2021 10.75 9.3 1.13 2025-07-30 ⓘ 14 15 magnetic iron oxide nanoparticles; melittin; intracranial arterial dolichoectasia; macrophages; extracellular matrix; matrix metalloproteinases ⓘ NF-KAPPA-B; VERTEBROBASILAR DOLICHOECTASIA; MATRIX METALLOPROTEINASES; BASILAR ARTERY; DILATATIVE ARTERIOPATHY; TRIGEMINAL NEURALGIA; NATURAL-HISTORY; BEE VENOM; ANEURYSMS; MATRIX-METALLOPROTEINASE-9 ⓘ Extracellular matrix; Intracranial arterial dolichoectasia; Macrophages; Magnetic iron oxide nanoparticles; Matrix metalloproteinases; Melittin ⓘ Animals; Antigens, CD; Antigens, Differentiation, Myelomonocytic; Cerebral Arteries; Cerebrovascular Disorders; Chemokine CCL2; Disease Models, Animal; Inflammation; Macrophages; Magnetite Nanoparticles; Male; Matrix Metalloproteinase 9; Melitten; Mice; Mice, Inbred C57BL; NF-kappa B; Tumor Necrosis Factor-alpha; Ccl2 protein, mouse; CD68 protein, mouse; differentiation antigen; gelatinase B; immunoglobulin enhancer binding protein; leukocyte antigen; magnetite nanoparticle; melittin; monocyte chemotactic protein 1; tumor necrosis factor; animal; brain artery; C57BL mouse; cerebrovascular disease; disease model; inflammation; macrophage; male; metabolism; mouse; pathology; physiology ⓘ English 2021 2021 10.7150/ijbs.60588 ⓘ 바로가기 바로가기 바로가기 바로가기
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