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| WoS | SCOPUS | Document Type | Document Title | Abstract | Authors | Affiliation | ResearcherID (WoS) | AuthorsID (SCOPUS) | Author Email(s) | Journal Name | JCR Abbreviation | ISSN | eISSN | Volume | Issue | WoS Edition | WoS Category | JCR Year | IF | JCR (%) | FWCI | FWCI Update Date | WoS Citation | SCOPUS Citation | Keywords (WoS) | KeywordsPlus (WoS) | Keywords (SCOPUS) | KeywordsPlus (SCOPUS) | Language | Publication Stage | Publication Year | Publication Date | DOI | JCR Link | DOI Link | WOS Link | SCOPUS Link |
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| ○ | ○ | Article | Characterization and modeling of weathering degradation of PC/ABS blend in various temperature and humidity conditions | In this study, the accelerated degradation tests were conducted on PC/ABS blend (50:50) under various combinations of temperature and humidity, 85 degrees C-85 %RH, 85 degrees C-45 %RH, 75 degrees C-65 %RH, 85 degrees C-dry, 95 degrees C-dry, and 105 degrees C-dry conditions. The generation of surface damages were observed in detail, and a quantitative degree of degradation was defined based on the spectroscopic analysis. Also, the master curves correlating the degree of degradation with mechanical properties were constructed. The prediction model for degradation degree and mechanical properties under arbitrary temperature and humidity condition was suggested based on the degradation kinetics, and it was utilized to estimate the degradation behavior of field-weathered samples for 6 months. Based on this study, it is believed that the degradation behavior of PC/ABS blend under a wide range of weathering conditions can be predicted accurately by suggested protocol, and the reliable application potential of PC/ABS blends for various industrial areas is enhanced. | Kim, Na-Im; Lee, Jeong-Moo; Moon, Jong-Sin; Wee, Jung-Wook | Kyungpook Natl Univ, Coll Engn, Sch Mech Engn, 80 Daehak Ro, Daegu 41566, South Korea; LG Chem Ltd, Engn Mat Div, 211 Hwangsae Ro, Osan Si 18126, Gyeonggi Do, South Korea; LG Magna e Powertrain Co Ltd, 322 Gyeongmyeong Daero, Incheon 22744, South Korea | 59326862400; 55737394200; 59542167000; 56673775300 | jwwee@knu.ac.kr; | POLYMER DEGRADATION AND STABILITY | POLYM DEGRAD STABIL | 0141-3910 | 1873-2321 | 238 | SCIE | POLYMER SCIENCE | 2024 | 7.4 | 6.9 | 0 | 2025-05-07 | 0 | 0 | PC/ABS blend; Accelerated degradation; Weathering; FT-IR spectroscopy; Lifetime estimation | ACRYLONITRILE-BUTADIENE-STYRENE; THERMAL-DEGRADATION; ABS; OXIDATION; OUTDOOR; PHOTOOXIDATION; POLYCARBONATE; POLYBUTADIENE; RADIATION; RUBBER | Accelerated degradation; FT-IR spectroscopy; Lifetime estimation; PC/ABS blend; Weathering | 'Dry' [; Accelerated degradation; Degree of degradation; FT-IR spectroscopy; Humidity conditions; IR-spectroscopy; Lifetime estimation; PC/ABS blend; Temperature and humidities; Temperature conditions | English | 2025 | 2025-08 | 10.1016/j.polymdegradstab.2025.111364 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |||
| ○ | ○ | Article | Dietary red bean seedlings extract alleviates obesity via activation of PPARα - AMPKα signaling in white adipose tissue of high-fat diet-fed obese mice | With the global prevalence of obesity rising, there is an increasing need for the development of obesity treatments using natural substances with fewer side effects. The efficacy of germinated red bean extract and its bioactive compound, azukisaponin II (AZ), on anti-obesity has been reported very little to date. This study aims to investigate the anti-obesity effects of red bean seedling extract (RS) and AZ, on PPAR alpha and AMPK alpha signaling pathways in white adipose tissue. RS supplementation effectively reduced fat mass and improved lipid metabolism in HFD-induced obese mice. RS decreased body weight gain, reduced adipocyte size, and lowered plasma triglyceride, free fatty acids, and total cholesterol. RS also enhanced mitochondrial function and fatty acid oxidation by activating AMPK alpha signaling and upregulating PPAR alpha expression in white adipose tissue. In particular, the levels of lipolysis-related factors (ATGL, HSL, and PLIN5) and proteins in the mitochondrial electron transport chain (NDUFB8, SDHB, UQCRC2, MTCO1, ATP5A) were increased in the RS200 and RS300 groups. RS and AZ treatments inhibited adipogenesis and promoted lipid metabolism in 3T3-L1 adipocytes. Additionally, we confirmed that treating PPAR alpha-knockdown 3T3-L1 cells with RS and AZ alleviates lipid accumulation by activating PPAR alpha-AMPK alpha signaling. RS supplementation effectively reduces obesity in HFD-induced mice by enhancing lipid metabolism and mitochondrial function through PPAR alpha-AMPK alpha signaling. Additionally, RS and AZ decrease lipid accumulation and promote mitochondrial biogenesis in 3T3-L1 cells, indicating their potential for treating obesity and metabolic disorders with a favorable safety profile. | Jang, Hisu; Shin, Su-Kyung; Bae, Heekyong R.; Lee, Hangyeol; Seo, Hye-Young; Seo, Woo Duck; Kwon, Eun-Young | Kyungpook Natl Univ, Dept Food Sci & Nutr, 80 Daehak Ro, Daegu 41566, South Korea; Kyungpook Natl Univ, Ctr Food & Nutr Genom Res, 80 Daehak Ro, Daegu 41566, South Korea; Rural Dev Adm RDA, Natl Inst Crop Sci NICS, Food Tech Resources Res Div, Wanju 55365, South Korea; Rural Dev Adm RDA, Int Technol Cooperat Ctr ITCC, Cheorwon Branch, Jeonrabug Do 54875, South Korea; Kyungpook Natl Univ, Ctr Beautiful Aging, 80 Daehak Ro, Daegu 41566, South Korea; Kyungpook Natl Univ, Dept Integrat Bioconvergence, 80 Daehak Ro, Daegu 41566, South Korea | 59946326600; 23988149900; 57191253762; 58054795600; 58304229500; 59894541500; 15765422500 | swd2002@korea.kr; eykwon@knu.ac.kr; | FOOD RESEARCH INTERNATIONAL | FOOD RES INT | 0963-9969 | 1873-7145 | 218 | SCIE | FOOD SCIENCE & TECHNOLOGY | 2024 | 8 | 6.9 | N/A | 0 | 0 | AMPK alpha; Azukisaponin II; Obesity; PPAR alpha; Red bean seedlings extract; White adipose tissue | GAMMA | AMPKα; Azukisaponin II; Obesity; PPARα; Red bean seedlings extract; White adipose tissue | Chemical activation; Dietary supplements; Fatty acids; Mammals; Metabolism; Mitochondria; Physiology; Plants (botany); Seed; Tissue; AMPKα; Azukisaponin II; Lipid metabolisms; Mitochondrial function; Obese mice; Obesity; PPARα; Red bean seedling extract; Red beans; White adipose tissues; Nutrition | English | 2025 | 2025-10 | 10.1016/j.foodres.2025.116803 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||||
| ○ | ○ | Article | In vitro temperature-dependent degradation of polylactic acid: Experimental characterization and neural network-based prediction | To ensure the reliable use of polylactic acid (PLA) as a medical polymer, it is essential to develop a model that can accurately assess the degradation of its mechanical properties under in vitro conditions. In this study, PLA was immersed in phosphate buffered saline at 37 degrees C to investigate its degradation behavior. A marked decline in tensile strength was observed following an induction period, with surface analysis revealing that diffusion-limited hydrolysis (DLH) characterized by the formation of hydrolysis-induced micro-pits and cracks was the primary degradation mechanism. Chain scission and crystallization behavior resulting from hydrolysis were systematically examined. Using experimental data obtained at elevated temperatures (50, 60, and 70 degrees C), crystallization kinetics were modeled using the Avrami equation, enabling the development of a predictive relationship between tensile strength and crystallinity. Furthermore, an artificial neural network was employed to construct a model capable of accurately predicting tensile strength across a range of temperatures and exposure durations, thereby enhancing the reliability of PLA for in vitro applications. | Woo, Soo-Hyun; Kim, Na-Im; Wee, Jung-Wook | Kumoh Natl Inst Technol, Dept Mech Engn, 61 Daehak Ro, Gumi 39177, Gyeongbuk, South Korea; Kyungpook Natl Univ, Sch Mech Engn, 80 Daehak Ro, Daegu 41566, South Korea | 58954184700; 59326862400; 56673775300 | jwwee@knu.ac.kr; | POLYMER DEGRADATION AND STABILITY | POLYM DEGRAD STABIL | 0141-3910 | 1873-2321 | 240 | SCIE | POLYMER SCIENCE | 2024 | 7.4 | 6.9 | 0 | 0 | Poly lactic acid; Temperature-dependent hydrolysis; Phosphate-buffered saline; Avrami model; Artificial neural network | HYDROLYTIC DEGRADATION; POLY(LACTIC ACID); CRYSTALLIZATION KINETICS; BIODEGRADABLE POLYMERS; MECHANICAL-PROPERTIES; PLA; STEREOCOMPLEX; PARAMETERS; MORPHOLOGY; BEHAVIOR | Artificial neural network; Avrami model; Phosphate-buffered saline; Poly lactic acid; Temperature-dependent hydrolysis | Biodegradation; Crystallinity; Crystallization kinetics; Degradation; Hydrolysis; Lactic acid; Surface analysis; Tensile strength; Avrami models; Experimental characterization; In-vitro; Medical polymers; Network-based; Neural-networks; Phosphate-buffered salines; Poly lactic acid; Temperature dependent; Temperature-dependent hydrolysis; Neural networks | English | 2025 | 2025-10 | 10.1016/j.polymdegradstab.2025.111497 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |||||
| ○ | Article | Integrating geostatistical methods and deep learning for enhanced 87Sr/86Sr isoscape Estimation: A case study in South Korea | The 87Sr/86Sr isotopic ratio has emerged as a valuable geochemical tracer in fields such as environmental forensics, archaeology, and provenance research. However, generating accurate and spatially continuous isoscape maps from sparse isotopic measurements remains a major challenge due to limited data availability and spatial heterogeneity. To address this, we propose a hybrid framework for 87Sr/86Sr isoscape mapping that integrates a kriging-based data augmentation method with a deep learning (DL) classifier. The kriging component generates synthetic training samples by interpolating sparse isotopic data while preserving underlying spatial correlations and geological anisotropy. These augmented data, along with spatial geological features (e.g., lithology, tectonic settings) and geochemical compositions, are used as input variables for training a feedforward deep neural network. The approach was applied to 409 soil samples collected across South Korea, and its performance was benchmarked against conventional kriging and convolutional neural networks (CNN). The proposed model achieved significantly higher classification accuracy (91.67%) compared to kriging-based and CNN-based models (76.7% and 86.7%, respectively). Furthermore, the isoscape outputs revealed meaningful isotopic patterns linked to geological and geomorphological controls, such as metamorphic rock distributions, fault density, and surface slope. This framework demonstrates the effectiveness of combining geostatistics with DL to improve predictive accuracy and interpretability in isotopic provenance research and environmental monitoring. © 2025 The Authors | Lee, Hyeongmok; Kim, Go-Eun; Shin, Woo-Jin; Lee, Yuyoung; Park, Sanghee; Lee, Kwang-Sik; Jeong, Jina; Park, Seung-Ik; Choung, Sungwook | Department of Geology, Kyungpook National University, Daegu, 41566, Cuba; of Analytical Science and Technology, Chungnam National University, Daejeon, 34134, Cuba, Geoanalysis Center, Korea Institute of Geoscience and Mineral Resources, Daejeon, 34132, Cuba; Research Center of Earth and Environmental Sciences, Korea Basic Science Institute, Cheongju, 28119, Cuba; Research Center of Earth and Environmental Sciences, Korea Basic Science Institute, Cheongju, 28119, Cuba; Research Center of Earth and Environmental Sciences, Korea Basic Science Institute, Cheongju, 28119, Cuba; of Analytical Science and Technology, Chungnam National University, Daejeon, 34134, Cuba, Research Center of Earth and Environmental Sciences, Korea Basic Science Institute, Cheongju, 28119, Cuba; Department of Geology, Kyungpook National University, Daegu, 41566, Cuba; Department of Geology, Kyungpook National University, Daegu, 41566, Cuba; Research Center of Earth and Environmental Sciences, Korea Basic Science Institute, Cheongju, 28119, Cuba, Department of Environmental System Engineering, Korea University, Sejong, 30019, Cuba | 58306661200; 59965709400; 7202123718; 59966249500; 57204054900; 55737516500; 55488558800; 55832472000; 36436826400 | jeong.j@knu.ac.kr; | International Journal of Applied Earth Observation and Geoinformation | INT J APPL EARTH OBS | 1569-8432 | 1872-826X | 142 | SCIE | REMOTE SENSING | 2024 | 8.6 | 6.9 | 0 | Deep learning; Geostatistical data augmentation method; Secondary information; Strontium isotope ratio; Uncertainty estimation | South Korea; geostatistics; isotopic ratio; machine learning; strontium isotope; uncertainty analysis | English | Final | 2025 | 10.1016/j.jag.2025.104697 | 바로가기 | 바로가기 | 바로가기 | ||||||||||
| ○ | Article | Iso-dibenzo[g,p]chrysene-Fused Bis-dicarbaporphyrin: Pd Coordination-Induced Global Aromaticity and Stabilization of Double In-Plane Pd(IV) Cations | An iso-dibenzo[g,p]chrysene (DBC)-fused bis-dicarbaporphyrin (4) with two adj-CCNN cores was synthesized. This bis-dicarbaporphyrin can be regarded as an isomer of the previously reported DBC-fused bis-dicarbacorrole obtained by tuning the orientation of the DBC bridge. This subtle change has significant impacts on structure and properties. As prepared, 4 has a highly twisted figure-eight conformation, whereas DBC-fused bis-dicarbacorrole adopts roughly a planar conformation with slight distortion. The incorporation of two Pd(II) cations can induce a transformation in the molecular framework, resulting in a curved, arch-shaped pi-system. Utilizing crystallographic data, along with the electron density of delocalized bonds (EDDB), electron localization function-pi (ELF-pi), harmonic oscillator model of aromaticity (HOMA), the anisotropy of the induced current density (ACID), and nucleus-independent chemical shift (NICS) calculations, a 34 pi-electron global aromatic pathway was elucidated for the bis-Pd(II) complex (5). The Pd coordination-induced global aromaticity is a distinctive feature that has not been observed in the previously reported bis-Pd complexes of DBC-fused bis-dicarbacorrole. Moreover, the treatment of 5 with CuCl2 leads to the formation of a rare in-plane bis-Pd(IV) complex 6 with two axial coordinated Cl anions. The bis-Pd(IV) complex retains the global aromatic feature and arch-shaped conformation. To our knowledge, this is the first well-defined in-plane bis-Pd(IV) organometallic complex using a porphyrinoid-type macrocyclic ligand. | Zong, Zhaohui; Zhang, Xiaotong; Liu, Ningchao; Jung, Sang Mok; Oh, Juwon; Ke, Xian-Sheng | Beijing Normal Univ, Coll Chem, Beijing 100875, Peoples R China; Soonchunhyang Univ, Res Inst Basic Sci, Asan 31538, South Korea; Soonchunhyang Univ, Dept Chem, Asan 31538, South Korea; Kyungpook Natl Univ, Dept Chem, Daegu 41566, South Korea | Ke, Xian-Sheng/A-4608-2019 | kexiansheng@bnu.edu.cn; | JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | J AM CHEM SOC | 0002-7863 | 1520-5126 | 147 | 15 | SCIE | CHEMISTRY, MULTIDISCIPLINARY | 2024 | 15.6 | 6.9 | 0 | ELECTRON LOCALIZATION FUNCTION; INDEPENDENT CHEMICAL-SHIFTS; PORPHYRIN ANALOGS; SUBUNIT; DENSITY; RING | English | 2025 | 2025-04-07 | 10.1021/jacs.5c01135 | 바로가기 | 바로가기 | 바로가기 | ||||||||||
| ○ | ○ | Article | Jerusalem artichoke extracts regulate the gene expression of key enzymes involved in fatty acid biosynthesis | Jerusalem artichoke (JA) is a traditional remedy for alleviating symptoms of diabetes. In fact, the suppressive effects of JA on blood sugar have been reported in multiple studies since 1934. Recent studies have indicated that type II diabetes is often caused by insulin resistance rather than insulin reduction and that increased blood and interstitial fatty acid levels contribute to insulin resistance and the development of diabetes. However, whether JA affects human lipogenesis has not been studied. Here, we elucidated the effects of JA on the expression of two key enzymes involved in fatty acid biosynthesis, fatty acid synthase (FASN) and acetyl-CoA carboxylase (ACACA), using three human neuroblastoma and colon and liver cancer cell lines. Caffeine and ICRF193, a catalytic inhibitor of topoisomerase II (TOP2), were included as positive controls, and JA was extracted into water- or dimethyl sulfoxide-soluble components, termed H-JA and D-JA. Metabolomics analyses using gas chromatography-time of flight/mass spectrometry and in-silico analyses showed differentially enriched chemical compounds in H-JA and D-JA, suggesting their distinctive bioactivities including fatty acid metabolism. D-JA significantly reduced the expression of FASN and ACACA at the mRNA and protein levels. D-JA-treated cells exhibited altered TOP2 levels and FASN/ACACA expression appeared to be controlled by TOP2 activity and levels. Taken together, our study revealed a novel effect of JA extracts on inhibiting the expression of the key enzymes involved in the fatty acid biosynthesis and suggested the potential of JA as a natural medicinal agent to control lipogenesis in humans. | Lee, Soo Jin; Shin, Woo-Cheol; Ju, Sangmin; Gwon, Mi-Ri; Lee, Jae-Hwa; Yoon, Young-Ran; Calderwood, Stuart K.; Lee, Dae Young; Bunch, Heeyoun | Kyungpook Natl Univ, Coll Agr & Life Sci, Sch Appl Biosci, Daegu 41566, South Korea; Kyungpook Natl Univ, Dept Biomed Convergence Sci & Technol, Daegu 41566, South Korea; Kyungpook Natl Univ, Sch Life Sci, FOUR KNU Creat BioRes Grp BK21, Daegu 41566, South Korea; Kyungpook Natl Univ, Clin Om Inst, Sch Med, Daegu 41944, South Korea; Kyungpook Natl Univ, Sch Med, Daegu 41944, South Korea; Harvard Med Sch, Dept Radiat Oncol, Beth Israel Deaconess Med Ctr, Boston, MA 02115 USA; Kyungpook Natl Univ, Dept Appl Biosci, Daegu 41566, South Korea; Hayden Rowe Writing Cooperat, Hopkinton, MA 01748 USA | Bunch, Heeyoun/JAX-3215-2023 | 59654765500; 57212601607; 58828036200; 56035800800; 58165025100; 14629744500; 7103094191; 57750904900; 56336812200 | heeyounbunch@gmail.com; | JOURNAL OF AGRICULTURE AND FOOD RESEARCH | J AGR FOOD RES | 2666-1543 | 21 | ESCI | AGRICULTURE, MULTIDISCIPLINARY;FOOD SCIENCE & TECHNOLOGY | 2024 | 6.2 | 6.9 | 0 | 2025-05-07 | 0 | 0 | Gene regulation; Jerusalem artichoke; Functional food; Fatty acid biosynthesis; Fatty acid synthase; Acetyl-coA carboxylase; Topoisomerase II | INSULIN-RESISTANCE; METABOLIC SYNDROME; DISEASE; INHIBITION; OXIDATION | Acetyl-coA carboxylase; Fatty acid biosynthesis; Fatty acid synthase; Functional food; Gene regulation; Jerusalem artichoke; Topoisomerase II | English | 2025 | 2025-06 | 10.1016/j.jafr.2025.101819 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||||
| ○ | ○ | Article | Load factor evaluation and emissions calculation for the cultivator under real working conditions | Currently, emission management for agricultural machinery in South Korea only targets diesel engines, leading to insufficient management of gasoline engines. Emission calculations are required to manage emissions, and a load factor (LF) is necessary for these calculations. However, there is currently no standard for the LF for gasoline engines in South Korea. Therefore, the purpose of this study was to measure the engine LF for the small multipurpose cultivator under actual working conditions and evaluate it by comparison with the national Clean Air Policy Support System (CAPSS) of South Korea and the European Environment Agency (EEA) approaches, respectively. A torque sensor capable of collecting torque and rotational speed was installed on the engine output shaft, and a data acquisition board was used to collect data. Field tests were conducted on the main operations of the cultivator, such as idling, rotary tillage, ditching, and ridging operations. Engine power was calculated using engine torque and rotational speed, and LF was calculated using real-time power and rated power. In addition, the unified LF was calculated by using the weight for each operation and the average LF for each operation. As a result, the unified LF of the cultivator was 0.65, which was different from that of CAPSS (diesel, 0.48) and EEA (gasoline, 0.40). As a result of calculating the annual emission of the cultivator through the unified LF derived from this study and emission factors of EEA, emissions for CO and VOC were found to be higher in the cultivator than in the tractor and combine harvesters currently being statistically collected in South Korea. The results of this study are expected to provide useful information for estimating the LF and emissions of small agricultural machines equipped with gasoline engines, such as cultivators. | Lee, Si-Eon; Beak, Seung-Min; Beak, Seung-Yun; Lim, Ryu-Gap; Kim, Yong-Joo; Kim, Wan-Soo | Kyungpook Natl Univ, Dept Bioind Machinery Engn, Daegu 41566, South Korea; Chungnam Natl Univ, Dept Biosyst Machinery Engn, Daejeon 34134, South Korea; Chungnam Natl Univ, Ecofriendly Hydrogen Elect Tractor & Agr Machinery, Daejeon, South Korea; Sunchon Natl Univ, Dept Convergence Biosyst Engn, Sunchon 57922, South Korea; Dept Smart Bioind Mech Engn, Daegu 41566, South Korea; Kyungpook Natl Univ, Upland Field Machinery Res Ctr, Daegu 41566, South Korea | 59515486400; 59185662700; 57204038124; 57216499874; 57204759454; 57192918810 | babina@cnu.ac.kr; wansoo.kim@knu.ac.kr; | JOURNAL OF AGRICULTURE AND FOOD RESEARCH | J AGR FOOD RES | 2666-1543 | 19 | ESCI | AGRICULTURE, MULTIDISCIPLINARY;FOOD SCIENCE & TECHNOLOGY | 2024 | 6.2 | 6.9 | 0 | 2025-05-07 | 0 | 0 | Cultivator; Load factor; Actual working condition; Small gasoline engine | Actual working condition; Cultivator; Load factor; Small gasoline engine | English | 2025 | 2025-03 | 10.1016/j.jafr.2025.101636 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||||||
| ○ | Article | Utilizing Leuconostoc mesenteroides C6 as a starter culture to improve flatfish-Sikhae fermentation stability and quality | Sikhae is a traditional Korean fermented fish product rich in microbial diversity and flavor. The fermentation process of Sikhae often yields unstable and variable quality. To address this, this study aimed to evaluate the potential of lactic acid bacteria (LAB) as a starter culture for flatfish-Sikhae fermentation in terms of fermentation stability, microbial community dynamics, and quality improvement. Leuconostoc mesenteroides C6 showed high adaptability to fermentation conditions such as salinity up to 5.0 % and acidity as low as pH 3.0. L. mesenteroides C6 significantly reduced the pH, and increased acidity during the initial fermentation stages and maintained high LAB counts, maintaining a stable fermentation process. These physicochemical changes stabilized the fermentation conditions. Microbial community analysis revealed that L. mesenteroides C6 enhanced the dominance of Leuconostoc spp., resulting in a more repressed and stable microbial community. Starter C6 improved sensory characteristics, such as the disappearance of the fishy smell and enhancement in flavor and texture, resulting in an overall preference. In addition, based on the multivariate analysis between variables, a correlation analysis among these variables, such as microbial stability and quality indicators, was assessed. The findings of this study suggest L. mesenteroides C6 as a promising starter culture for enhancing the quality and fermentation of flatfish-Sikhae and provide novel insights into the application of LAB in traditional Korean fermented foods, opening new avenues for modern quality management, optimization of fermentation control, and industrialization. © 2025 Elsevier Ltd | Jo, Du-Min; Won, Dong-Hoon; Yeo, Sohyun; Lee, Seungjun; Kim, Young-Mog | National Marine Biodiversity Institute of Korea, Seochun, Chungcheongnam-do, 33662, South Korea; Food Microbiology Division, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety, Cheongju, 28159, South Korea; Department of Food Science and Nutrition, Pukyong National University, Busan, 48513, South Korea; Department of Applied Biosciences, Kyungpook National University, Daegu, 41566, South Korea; Department of Food Science and Technology, Pukyong National University, Busan, 48513, South Korea | 57219718893; 59956482900; 59510841500; 59947332300; 59655786600 | ymkim@pknu.ac.kr; | Food Research International | FOOD RES INT | 0963-9969 | 1873-7145 | 218 | SCIE | FOOD SCIENCE & TECHNOLOGY | 2024 | 8 | 6.9 | 0 | Fermentation starter; Lactic acid bacteria; Leuconostoc mesenteroides; Sikhae | Bacteria; Fermentation; Fish products; Food processing; Multivariant analysis; Process control; Quality management; Sensory perception; Starters; Textures; Fermentation conditions; Fermentation process; Fermentation starter; Fermented fishes; Lactic acid bacteria; Leuconostoc mesenteroides; Microbial community dynamics; Microbial diversity; Sikhae; Starter cultures; Lactic acid | English | Final | 2025 | 10.1016/j.foodres.2025.116937 | 바로가기 | 바로가기 | 바로가기 | ||||||||||
| ○ | ○ | Article | Wild and cultivated Ecklonia cava: A comparative study of metabolites and bioactivities for industrial applications | Ecklonia cava, an East Asian brown algae, is valued for its antioxidant and anti-inflammatory metabolites. This study is the first to investigate the metabolite profiles and biological activities of cultivated E. cava (CE) in comparison with those of wild E. cava (WE). A 50 % ethanol extract prepared from WE (WECE) and CE (CECE) was used for comparative analysis. WECE showed higher total polyphenolic content and antioxidant activity than CECE, while both extracts demonstrated similar anti-inflammatory activity. UPLC-QTOF-MS/MS analysis revealed the CECE contains key polyphenolic metabolites that are also present in WE, including eckol, 6,6 ' bieckol, dieckol, and phlorofucofuroeckol-A. A total of 148 differential metabolites were identified, among which eight matairesinol, 2-protocatechuyl phloroglucinol carboxylic acid, 5-methyl-3-[(11Z,15Z)-11,15-tricosadien-1yl]-2(5H)-furanone, neoacrimarine E, glycyl-alpha-aspartylglycylalanyl-asparaginylalanylglutaminate, pteroyltyrosine, kosamol A, and daucosterol were strongly correlated with both antioxidant and anti-inflammatory activities and exhibited similar compositional patterns in both extracts. Furthermore, correlation analysis of the overall metabolite composition between WECE and CECE indicated no significant differences. These results suggest that, despite the lower extraction yield and total phenolic content, CECE retains bioactive components comparable to WECE. These findings suggest that CE, which shows comparable metabolite composition and bioactivity to its wild counterpart, holds strong potential as a sustainable source for the industrial production of antioxidant and anti-inflammatory agents. | Lee, Sang-Woon; Hyun, Jimin; Je, Jun-Geon; Kang, Dayun; Amarasiri, Rajasinghe Peli Gedara Sewwandi Kaushalya; Lee, Seungjun; Boo, Sung Min; Choi, Chang Geun; Lee, Ju Il; Ryu, Bomi; Jeon, You-Jin | Jeju Natl Univ, Dept Marine Life Sci, Jeju 63253, South Korea; Pukyong Natl Univ, Dept Food Sci & Nutr, Busan 48513, South Korea; Kyungpook Natl Univ, Dept Appl Biosci, Daegu 41566, South Korea; Chungnam Natl Univ, Dept Biol Sci, Daejeon 34134, South Korea; Pukyong Natl Univ, Dept Ecol Engn, Busan 48513, South Korea | Jeon, You-Jin/AAD-3452-2021; Boo, Sung/F-5734-2013 | 58159242900; 57217067683; 59946416600; 58493492700; 58402604800; 59947332300; 59946594200; 7402961166; 57211318905; 35307815200; 55782690600 | bmryu@pknu.ac.kr; youjinj@jejunu.ac.kr; | FOOD RESEARCH INTERNATIONAL | FOOD RES INT | 0963-9969 | 1873-7145 | 217 | SCIE | FOOD SCIENCE & TECHNOLOGY | 2024 | 8 | 6.9 | N/A | 0 | 0 | Ecklonia cava; Metabolite; Polyphenol; Anti-inflammation; Antioxidant; Correlation analysis | NATURAL-PRODUCTS | Anti-inflammation; Antioxidant; Correlation analysis; Ecklonia cava; Metabolite; Polyphenol | Algae; Bioactivity; Biomolecules; Correlation methods; Cultivation; Anti-inflammation; Anti-inflammatories; Anti-inflammatory activity; Brown algae; Comparatives studies; Correlation analysis; Ecklonia cava; Ethanol extract; Metabolite profiles; Polyphenols; Antioxidants; Metabolites | English | 2025 | 2025-10 | 10.1016/j.foodres.2025.116862 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |||
| ○ | ○ | Article | β,β-Dimethylacryloyl Alkannin from Arnebia euchroma Roots Suppresses Triple-Negative Breast Cancer Growth via AKT/Gli1 Signaling | After confirmation of Arnebia euchroma by genetic analysis, we identified the key compounds from the root: alkannin (1), acetylalkannin (2), beta-acetoxyisovaleryl alkannin (3), isobutyryl alkannin (4), and beta,beta-dimethylacryloyl alkannin (DMA) (5). Among these, DMA most effectively inhibited the proliferation of triple-negative breast cancer (TNBC) cells, with IC50 values of 5.1 mu M (MDA-MB-231) and 8.7 mu M (MCF10DCIS.com). DMA and the Hedgehog (Hh) inhibitor Gant61 targeting Gli1 significantly induced apoptosis, as indicated by increased Bax and cleaved PARP, and decreased Bcl-2 levels (p < 0.01) in both cell lines. We also identified AKT as a potential target of DMA, as treatment reduced phosphorylated AKT (Ser473) protein levels by 66.3% +/- 0.7% and 30.1% +/- 5.7% in MDA-MB-231 and MCF10DCIS.com cells, respectively (p < 0.01). In vivo, DMA (25 mg/kg) suppressed MDA-MB-231 xenograft tumor growth by approximately 78% (p < 0.01) and induced apoptosis through regulating AKT/Hh/Gli1 axis. Interestingly, the reduced form of DMA (5 ') lost its efficacy in inhibiting proliferation, p-AKT expression, and Gli1 transcriptional activity and nuclear localization, indicating that the Michael acceptor in DMA is critical for inhibiting TNBC growth. Overall, DMA suppressed TNBC in vitro and in vivo through AKT/Gli1 pathway and shows potential as a potent agent against TNBC. | Zhou, Yimeng; Kim, Jin Tae; Kwon, Jung Won; Lee, Ga Yeon; Son, Hui Mang; Qiu, Shuai; Kim, Jaewon; Chi, Hae Na; Cao, Thao Quyen; Hahn, Dongyup; Lee, Hong Jin | Chung Ang Univ, Dept Food Sci & Biotechnol, Anseong 17546, South Korea; Chung Ang Univ, GreenTech Based Food Safety Res Grp, BK21 Four, Anseong 17546, South Korea; Tongji Univ, Shanghai Peoples Hosp 10, Dept Clin Lab Med, Shanghai 200072, Peoples R China; Kyungpook Natl Univ, Coll Agr & Life Sci, Sch Food Sci & Biotechnol, Daegu 41566, South Korea; Kyungpook Natl Univ, Dept Integrat Biotechnol, Daegu 41566, South Korea; Kyungpook Natl Univ, Inst Agr Sci & Technol, Daegu 41566, South Korea | 57218923138; 57200163432; 58023808600; 58236640400; 59903448600; 57204590855; 57213607555; 59903395800; 56937820500; 36554163400; 59903611400 | dohahn@knu.ac.kr; hongjin@cau.ac.kr; | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | J AGR FOOD CHEM | 0021-8561 | 1520-5118 | 73 | 22 | SCIE | AGRICULTURE, MULTIDISCIPLINARY;CHEMISTRY, APPLIED;FOOD SCIENCE & TECHNOLOGY | 2024 | 6.2 | 6.9 | 0 | 2025-06-11 | 0 | 0 | Arnebia euchroma; beta,beta-dimethylacryloylalkannin; hedgehog signaling; Gli1; AKT; triple-negativebreast cancer; TNBC xenograft; Michael acceptor | SONIC-HEDGEHOG; PATHWAY; ACTIVATION; APOPTOSIS; EXTRACTS; SHIKONIN | AKT; Arnebia euchroma; Gli1; hedgehog signaling; Michael acceptor; TNBC xenograft; triple-negative breast cancer; β,β-dimethylacryloylalkannin | Animals; Antineoplastic Agents, Phytogenic; Apoptosis; Boraginaceae; Cell Line, Tumor; Cell Proliferation; Female; Humans; Mice; Mice, Inbred BALB C; Mice, Nude; Naphthoquinones; Plant Extracts; Plant Roots; Proto-Oncogene Proteins c-akt; Signal Transduction; Triple Negative Breast Neoplasms; Zinc Finger Protein GLI1; Cell death; Cell proliferation; Glycoproteins; alkannin; antineoplastic agent; GLI1 protein, human; naphthoquinone; plant extract; protein kinase B; transcription factor Gli1; AKT; Arnebia euchroma; Breast cancer xenografts; Gli1; Hedgehog signalling; Michael acceptors; Triple-negative breast cancer xenograft; Triple-negative breast cancers; Β,β-dimethylacryloylalkannin; animal; apoptosis; Bagg albino mouse; Boraginaceae; cell proliferation; chemistry; drug effect; drug therapy; female; genetics; human; metabolism; mouse; nude mouse; pathophysiology; plant root; signal transduction; triple negative breast cancer; tumor cell line; Tissue culture | English | 2025 | 2025-05-20 | 10.1021/acs.jafc.5c05537 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||
| ○ | ○ | Article | Activation of lysophagy by a TBK1-SCFFBXO³-TMEM192-TAX1BP1 axis in response to lysosomal damage | Lysophagy eliminates damaged lysosomes and is crucial to cellular homeostasis; however, its underlying mechanisms are not entirely understood. We screen a ubiquitination-related compound library and determine that the substrate recognition component of the SCF-type E3 ubiquitin ligase complex, SCFFBXO3(FBXO3), which is a critical lysophagy regulator. Inhibition of FBXO3 reduces lysophagy and lysophagic flux in response to L-leucyl-L-leucine methyl ester (LLOMe). Furthermore, FBXO3 interacts with TMEM192, leading to its ubiquitination in LLOMe-treated cells. We also identify TAX1BP1 as a critical autophagic adaptor that recognizes ubiquitinated TMEM192 during lysophagy and find that TBK1 activation is crucial for lysophagy, as it phosphorylates FBXO3 in response to lysosomal damage. Knockout of FBXO3 significantly impairs lysophagy, and its reconstitution with a loss-of-function mutant (V221I) further confirms its essential role in lysophagy regulation. Collectively, our findings highlight the significance of the TBK1-FBXO3-TMEM192-TAX1BP1 axis in lysophagy and emphasize the critical role of FBXO3 in lysosomal integrity. | Park, Na Yeon; Jo, Doo Sin; Yang, Jae-Yoon; Bae, Ji-Eun; Kim, Joon Bum; Kim, Yong Hwan; Kim, Seong Hyun; Kim, Pansoo; Lee, Dong-Seok; Yoshimori, Tamotsu; Jo, Eun-Kyeong; Yeom, Eunbyul; Cho, Dong-Hyung | Kyungpook Natl Univ, Sch Life Sci, BK21 FOUR KNU Creat BioRearch Grp, Daegu, South Korea; Kyungpook Natl Univ, Organelle Inst, Daegu, South Korea; ORGASIS Corp 260, Suwon, South Korea; Kyungpook Natl Univ, KNU Inst Basic Sci, Coll Nat Sci, KNU G LAMP Project Grp, Daegu, South Korea; Osaka Univ, Grad Sch Med, Dept Genet, Osaka, Japan; Chungnam Natl Univ, Coll Med, Dept Microbiol, Daejeon, South Korea | Yang, Jaeyoon/LYP-2093-2024; Yoshimori, Tamotsu/K-9626-2014; Kim, Joon/ADP-8066-2022 | 57190609826; 56335489800; 58954317200; 57190605352; 57190611030; 57204676401; 57253406700; 59548676400; 57210068061; 7006810223; 7003663754; 56058004100; 58950702200 | yeb@knu.ac.kr; dhcho@knu.ac.kr; | NATURE COMMUNICATIONS | NAT COMMUN | 2041-1723 | 16 | 1 | SCIE | MULTIDISCIPLINARY SCIENCES | 2024 | 15.7 | 7.0 | 5.89 | 2025-05-07 | 2 | 2 | MEMBRANE-PROTEINS; UBIQUITIN LIGASE; AUTOPHAGY; BIOGENESIS; TRAFFICKING; MECHANISM; PATHWAY; TMEM192; CELLS | Animals; Autophagy; F-Box Proteins; HEK293 Cells; HeLa Cells; Humans; Intracellular Signaling Peptides and Proteins; Lysosomes; Membrane Proteins; Mice; Neoplasm Proteins; Phosphorylation; Protein Serine-Threonine Kinases; Signal Transduction; SKP Cullin F-Box Protein Ligases; Ubiquitination; binding protein; F box protein 3; leucylleucine methyl ester; membrane protein; TANK binding kinase 1; tax1 binding protein 1; transmembrane protein 192; ubiquitin protein ligase E3; unclassified drug; F box protein; membrane protein; protein serine threonine kinase; signal peptide; TAX1BP1 protein, human; TBK1 protein, human; tumor protein; ubiquitin protein ligase; cell; ester; experimental study; recognition; substrate; animal tissue; Article; autophagic cell death; autophagy (cellular); cell damage; combinatorial library; controlled study; enzyme activation; enzyme inhibition; enzyme phosphorylation; gene knockout; human; human cell; loss of function mutation; lysosome; lysosome membrane; nonhuman; protein protein interaction; ubiquitination; animal; drug effect; genetics; HEK293S cell line; HeLa cell line; metabolism; mouse; phosphorylation; signal transduction | English | 2025 | 2025-01-28 | 10.1038/s41467-025-56294-y | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||||
| ○ | ○ | Article | Alternatively spliced mini-exon B in PTPδ regulates excitatory synapses through cell-type-specific trans-synaptic PTPδ-IL1RAP interaction | PTP delta, encoded by PTPRD, is implicated in various neurological, psychiatric, and neurodevelopmental disorders, but the underlying mechanisms remain unclear. PTP delta trans-synaptically interacts with multiple postsynaptic adhesion molecules, which involves its extracellular alternatively spliced mini-exons, meA and meB. While PTP delta-meA functions have been studied in vivo, PTP delta-meB has not been studied. Here, we report that, unlike homozygous PTP delta-meA-mutant mice, homozygous PTP delta-meB-mutant (Ptprd-meB-/-) mice show markedly reduced early postnatal survival. Heterozygous Ptprd-meB+/- male mice show behavioral abnormalities and decreased excitatory synaptic density and transmission in dentate gyrus granule cells (DG-GCs). Proteomic analyses identify decreased postsynaptic density levels of IL1RAP, a known trans-synaptic partner of meB-containing PTP delta. Accordingly, IL1RAP-mutant mice show decreased excitatory synaptic transmission in DG-GCs. Ptprd-meB+/- DG interneurons with minimal IL1RAP expression show increased excitatory synaptic density and transmission. Therefore, PTP delta-meB is important for survival, synaptic, and behavioral phenotypes and regulates excitatory synapses in cell-type-specific and IL1RAP-dependent manners. | Kim, Seoyeong; Shin, Jae Jin; Kang, Muwon; Yang, Yeji; Cho, Yi Sul; Paik, Hyojung; Kim, Jimin; Yi, Yunho; Lee, Suho; Koo, Hei Yeun; Bok, Jinwoong; Bae, Yong Chul; Kim, Jin Young; Kim, Eunjoon | Korea Adv Inst Sci & Technol KAIST, Dept Biol Sci, Daejeon 34141, South Korea; Inst Basic Sci IBS, Ctr Synapt Brain Dysfunct, Daejeon 34141, South Korea; Korea Basic Sci Inst KBSI, Digital Om Res Ctr, Ochang 28119, South Korea; Kyungpook Natl Univ, Sch Dent, Dept Anat & Neurobiol, Daegu 41940, South Korea; Korea Inst Sci & Technol Informat KISTI, Ctr Biomed Comp, Daejeon 34141, South Korea; Yonsei Univ, Coll Med, Dept Anat, Seoul 03722, South Korea | 57210113751; 56287477200; 57209347227; 57826103900; 59893178200; 15754227200; 57288025000; 57944162700; 57202691999; 57216653945; 25222398800; 59892984700; 59643434800; 57203240554 | kime@kaist.ac.kr; | NATURE COMMUNICATIONS | NAT COMMUN | 2041-1723 | 16 | 1 | SCIE | MULTIDISCIPLINARY SCIENCES | 2024 | 15.7 | 7.0 | 0 | 2025-06-11 | 0 | 0 | PHOSPHATASE-RECEPTOR-TYPE; LAR; ADHESION; NEUREXIN; DISORDER; SIGMA; ASSOCIATION; NEURONS; BINDING; CODE | Alternative Splicing; Animals; Behavior, Animal; Dentate Gyrus; Exons; Female; Interneurons; Male; Mice; Mice, Inbred C57BL; Mice, Knockout; Receptor-Like Protein Tyrosine Phosphatases, Class 2; Synapses; Synaptic Transmission; peptides and proteins; protein il1rap; protein tyrosine phosphatase; protein tyrosine phosphatase delta; unclassified drug; receptor like protein tyrosine phosphatase; abnormality; cell component; gene expression; nervous system disorder; protein; rodent; animal experiment; animal tissue; Article; cells by body anatomy; controlled study; dentate gyrus; exon; female; granule cell; interneuron; male; mouse; nerve cell; nerve ending; nonhuman; phenotype; postsynaptic density; protein interaction; survival; synapse; synaptic transmission; alternative RNA splicing; animal; animal behavior; C57BL mouse; cytology; genetics; knockout mouse; metabolism | English | 2025 | 2025-05-13 | 10.1038/s41467-025-59685-3 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | |||||
| ○ | ○ | Article | Anti-proteolytic regulation of KRAS by USP9X/NDRG3 in KRAS-driven cancer development | Cancers with activating mutations of KRAS show a high prevalence but remain intractable, requiring innovative strategies to overcome the poor targetability of KRAS. Here, we report that KRAS expression is post-translationally up-regulated through deubiquitination when the scaffolding function of NDRG3 (N-Myc downstream-regulated gene 3) promotes specific interaction between KRAS and a deubiquitinating enzyme, USP9X. In KRAS-mutant cancer cells KRAS protein expression, downstream signaling, and cell growth are highly dependent on NDRG3. In conditional KrasG12D knock-in mouse models of pancreatic ductal adenocarcinoma, Ndrg3 depletion abolishes Kras protein expression and suppresses intraepithelial neoplasia formation in pancreas. Mechanistically, KRAS protein binds to the C-terminal serine/threonine-rich region of NDRG3, subsequently going through deubiquitination by USP9X recruited to the complex. This interaction can be disrupted in a dominant-negative manner by a C-terminal NDRG3 fragment that binds KRAS but is defective in USP9X binding, highly suppressing KRAS protein expression and KRAS-driven cell growth. In summary, KRAS-driven cancer development critically depends on the deubiquitination of KRAS protein mediated by USP9X/NDRG3, and KRAS-addicted cancers could be effectively targeted by inhibiting the KRAS-NDRG3 interaction. | Koo, Han; Park, Kyung Chan; Sohn, Hyun Ahm; Kang, Minho; Kim, Dong Joon; Park, Zee-Yong; Park, Sehoon; Min, Sang Hyun; Park, Seong-Hwan; You, Yeon-Mi; Han, Yohan; Kim, Bo-Kyung; Lee, Chul-Ho; Kim, Yeon-Soo; Chung, Sang J.; Yeom, Young Il; Lee, Dong Chul | Korea Res Inst Biosci & Biotechnol KRIBB, Personalized Genom Med Res Ctr, Daejeon, South Korea; Univ Sci & Technol, KRIBB Sch Biosci, Dept Funct Genom, Daejeon, South Korea; Dankook Univ, Coll Med, Dept Microbiol, Cheonan, Chungcheongnam, South Korea; Dankook Univ, MRCRC, Cheonan, Chungcheongnam, South Korea; Gwangju Inst Sci & Technol, Sch Life Sci, Gwangju, South Korea; Kyungpook Natl Univ, Dept Innovat Pharmaceut Sci, Deagu, South Korea; Korea Res Inst Biosci & Biotechnol KRIBB, Lab Anim Resource Ctr, Daejeon, South Korea; Chungnam Natl Univ, Grad Sch New Drug Discovery & Dev, Daejeon, South Korea; Sungkyunkwan Univ, Sch Pharm, Dept Biopharmaceut Convergence, Suwon, Gyeonggi Do, South Korea; Chungnam Natl Univ, Coll Pharm, Daejeon, South Korea | Lee, Chul-Ho/MBV-8603-2025; Park, Jun/H-7127-2019; Chung, Sang/W-3005-2019; Kim, Dong Joon/KDN-8414-2024 | 56693870300; 7408065215; 24537812300; 56710836300; 57203012616; 6603940093; 57220924988; 7202852238; 37025059300; 56693714900; 59528876200; 55656043900; 56223516500; 57204139644; 23491702400; 57193599885; 57202974864 | yeomyi@kribb.re.kr; dclee@kribb.re.kr; | NATURE COMMUNICATIONS | NAT COMMUN | 2041-1723 | 16 | 1 | SCIE | MULTIDISCIPLINARY SCIENCES | 2024 | 15.7 | 7.0 | 0 | 2025-05-07 | 0 | 0 | PANCREATIC-CANCER; HIGH EXPRESSION; NDRG3; USP9X; METASTASIS; STABILITY; SURVIVAL; CONTRIBUTE; GENE-1 | Animals; Carcinoma, Pancreatic Ductal; Cell Line, Tumor; Cell Proliferation; Gene Expression Regulation, Neoplastic; Humans; Mice; Nerve Tissue Proteins; Pancreatic Neoplasms; Protein Binding; Proteolysis; Proto-Oncogene Proteins p21(ras); Ubiquitin Thiolesterase; Ubiquitination; binding protein; deubiquitinase; K ras protein; messenger RNA; mitogen activated protein kinase 1; mitogen activated protein kinase 3; n Myc downstream regulated gene 3; peptidase; proteasome; protein kinase B; scaffold protein; ubiquitin specific peptidase 9x linked; unclassified drug; Hras protein, mouse; KRAS protein, human; nerve protein; protein binding; protein p21; ubiquitin thiolesterase; USP9X protein, human; Usp9x protein, mouse; cancer; cell; enzyme activity; gene expression; growth response; mutation; protein; tumor; amino acid sequence; anchorage independent growth; animal experiment; animal model; animal tissue; Article; BxPC-3 cell line; cancer cell; cancer growth; cancer inhibition; Capan-1 cell line; carboxy terminal sequence; carcinogenesis; cell growth; cell mutant; cell proliferation; controlled study; deubiquitination; differential gene expression; disease free survival; ectopic expression; embryo; female; HEK293T cell line; human; human tissue; in vitro study; in vivo study; intraepithelial neoplasia; male; MIA PaCa-2 cell line; mouse; NCI-H358 cell line; nonhuman; pancreas tissue; pancreatic ductal carcinoma; protein degradation; protein depletion; protein expression; protein metabolism; protein phosphorylation; protein processing; protein protein interaction; protein stability; regulatory mechanism; signal transduction; tumor xenograft; ubiquitination; upregulation; animal; gene expression regulation; genetics; metabolism; pancreas tumor; pancreatic ductal carcinoma; pathology; protein degradation; tumor cell line; ubiquitination | English | 2025 | 2025-01-16 | 10.1038/s41467-024-54476-8 | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||||
| ○ | ○ | Article | Auto-sumoylation of the yeast Ubc9 E2 SUMO-conjugating enzyme extends cellular lifespan | Calorie restriction (CR) provides anti-aging benefits through diverse processes, such as reduced metabolism and growth and increased mitochondrial activity. Although controversy still exists regarding CR-mediated lifespan effects, many researchers are seeking interventions that mimic the effects of CR. Yeast has proven to be a useful model system for aging studies, including CR effects. We report here that yeast adapted through in vitro evolution to the severe cellular stress caused by loss of the Ulp2 SUMO-specific protease exhibit both enhanced growth rates and replicative lifespan, and they have altered gene expression profiles similar to those observed in CR. Notably, in certain evolved ulp2 Delta lines, an increase in the auto-sumoylation of Ubc9 E2 SUMO-conjugating enzyme results in altered regulation of multiple targets involved in energy metabolism and translation at both transcriptional and post-translational levels. This increase is essential for the survival of aged cells and CR-mediated lifespan extension. Thus, we suggest that high Ubc9 auto-sumoylation exerts potent anti-aging effects by promoting efficient energy metabolism-driven improvements in cell replication abilities. This potential could be therapeutically explored for the development of promising CR-mimetic strategies. | Jeong, Dong-Won; Lee, Do Yoon; Kim, Seung Yeon; Jeoung, Seok-Won; Zhao, Dejian; Knight, James; Lam, TuKiet T.; Jin, Jong Hwa; Lee, Hyun-Shik; Hochstrasser, Mark; Ryu, Hong-Yeoul | Kyungpook Natl Univ, KNU Inst Basic Sci, Coll Nat Sci, KNU G LAMP Res Ctr,Sch Life Sci,BK21 FOUR KNU Cre, Daegu, South Korea; Yale Univ, Yale Ctr Genome Anal, New Haven, CT USA; Yale Sch Med, Keck MS & Proteom Resource, New Haven, CT USA; Osong Med Innovat Fdn, New Drug Dev Ctr, Cheongju, South Korea; Yale Univ, Dept Mol Biophys & Biochem, New Haven, CT 06520 USA | Lam, TuKiet/B-6343-2008; Hoet, Peter/H-9987-2013 | 58987590500; 59136005700; 58987416100; 57822770000; 56145853100; 56988423500; 56903584900; 59440483400; 59858434400; 26643419800; 55889917800 | mark.hochstrasser@yale.edu; rhr4757@knu.ac.kr; | NATURE COMMUNICATIONS | NAT COMMUN | 2041-1723 | 16 | 1 | SCIE | MULTIDISCIPLINARY SCIENCES | 2024 | 15.7 | 7.0 | 0 | 2025-05-07 | 0 | 0 | SACCHAROMYCES-CEREVISIAE; CALORIE RESTRICTION; PROTEIN SUMOYLATION; ENERGY-METABOLISM; GENE ENCODES; TRANSCRIPTION; COMPLEX; SIR2; IDENTIFICATION; LOCALIZATION | Caloric Restriction; Cysteine Endopeptidases; Endopeptidases; Energy Metabolism; Gene Expression Regulation, Fungal; Saccharomyces cerevisiae; Saccharomyces cerevisiae Proteins; Sumoylation; Ubiquitin-Conjugating Enzyme UBC9; Ubiquitin-Conjugating Enzymes; proteinase; SUMO conjugating enzyme UBC9; cysteine proteinase; Saccharomyces cerevisiae protein; SUMO conjugating enzyme UBC9; ubiquitin conjugating enzyme; ULP2 protein, S cerevisiae; energy efficiency; enzyme activity; gene expression; growth rate; life cycle; metabolism; yeast; Article; caloric restriction; cell division; cell stress; cell survival; controlled study; energy metabolism; gene expression profiling; growth rate; in vitro study; life extension; nonhuman; regulatory mechanism; sumoylation; transcription regulation; translation regulation; yeast; enzymology; gene expression regulation; genetics; metabolism; Saccharomyces cerevisiae | English | 2025 | 2025-04-20 | 10.1038/s41467-025-58925-w | 바로가기 | 바로가기 | 바로가기 | 바로가기 | ||||
| ○ | Article | Bioresorbable, wireless dual stimulator for peripheral nerve regeneration | Wireless bioresorbable electrical stimulators have broad potential as therapeutic implants. Such devices operate for a clinically relevant duration and then harmlessly dissolve, eliminating the need for surgical removal. A representative application is in treating peripheral nerve injuries through targeted stimulation at either proximal or distal sites, with operation for up to one week. This report introduces enhanced devices with additional capabilities: (1) simultaneous stimulation of both proximal and distal sites, and (2) robust operation for as long as several months, all achieved with materials that naturally resorb by hydrolysis in surrounding biofluids. Systematic investigations of the materials and design aspects highlight the key features that enable dual stimulation and with enhanced stability. Animal model studies illustrate beneficial effects in promoting peripheral nerve regeneration, as quantified by increased total muscle and muscle fiber cross-sectional area and compound muscle action potentials. These findings expand the clinical applications of bioresorbable stimulators, particularly for long-term nerve regeneration and continuous neuromodulation-based monitoring. © The Author(s) 2025. | Ahn, Hak-Young; Walters, Jordan B.; Avila, Raudel; Oh, Seyong; Seo, Seung Gi; Kim, Jong Uk; Park, Jihun; Yoo, Seonggwang; Choi, Yeon Sik; Kim, Tae Yeon; Liu, Jiaqi; Yoo, Jae-Young; Weissleder, Oliver Ralph; D’Andrea, Dominic; Park, Chanho; Lee, Geumbee; Cho, Donghwi; Maeng, Woo-Youl; Yoon, Hong-Joon; Wickerson, Grace; Bouricha, Yasmine; Tian, Jing; Chung, Tzu Chun; Jordan, Sumanas W.; Li, Song; Huang, Yonggang; Franz, Colin K.; Rogers, John A. | Querrey Simpson Institute for Bioelectronics, Northwestern University, Evanston, 60208, IL, United States, Center for Bio-Integrated Electronics, Northwestern University, Evanston, 60208, IL, United States; Regenerative Neurorehabilitation Laboratory, Shirley Ryan AbilityLab, Chicago, IL, United States; Department of Mechanical Engineering, Rice University, Houston, 77005, TX, United States; Division of Electrical Engineering, Hanyang University ERICA, Ansan, 15588, South Korea; Querrey Simpson Institute for Bioelectronics, Northwestern University, Evanston, 60208, IL, United States, Center for Bio-Integrated Electronics, Northwestern University, Evanston, 60208, IL, United States; Querrey Simpson Institute for Bioelectronics, Northwestern University, Evanston, 60208, IL, United States, Center for Bio-Integrated Electronics, Northwestern University, Evanston, 60208, IL, United States; Querrey Simpson Institute for Bioelectronics, Northwestern University, Evanston, 60208, IL, United States, Center for Bio-Integrated Electronics, Northwestern University, Evanston, 60208, IL, United States; Querrey Simpson Institute for Bioelectronics, Northwestern University, Evanston, 60208, IL, United States, Center for Bio-Integrated Electronics, Northwestern University, Evanston, 60208, IL, United States, College of Biomedical Science and Health, Inje University, Gimhae, 50834, South Korea; Department of Materials Science and Engineering, Yonsei University, Seoul, 03722, South Korea; Querrey Simpson Institute for Bioelectronics, Northwestern University, Evanston, 60208, IL, United States, Center for Bio-Integrated Electronics, Northwestern University, Evanston, 60208, IL, United States; Querrey Simpson Institute for Bioelectronics, Northwestern University, Evanston, 60208, IL, United States, Center for Bio-Integrated Electronics, Northwestern University, Evanston, 60208, IL, United States; Department of Semiconductor Convergence Engineering, Sungkyunkwan University, Suwon, 16417, South Korea; Querrey Simpson Institute for Bioelectronics, Northwestern University, Evanston, 60208, IL, United States, Center for Bio-Integrated Electronics, Northwestern University, Evanston, 60208, IL, United States; Regenerative Neurorehabilitation Laboratory, Shirley Ryan AbilityLab, Chicago, IL, United States; Querrey Simpson Institute for Bioelectronics, Northwestern University, Evanston, 60208, IL, United States, Center for Bio-Integrated Electronics, Northwestern University, Evanston, 60208, IL, United States; Department of Chemical Engineering, Kyungpook National University, Daegu, 41566, South Korea; Thin Film Materials Research Center, Korea Research Institute of Chemical Technology, Daejeon, 34114, South Korea, Advanced Materials and Chemical Engineering, University of Science and Technology, Daejeon, 34113, South Korea; Querrey Simpson Institute for Bioelectronics, Northwestern University, Evanston, 60208, IL, United States, Center for Bio-Integrated Electronics, Northwestern University, Evanston, 60208, IL, United States; Department of Electronic Engineering, Gachon University, Seongnam, 13120, South Korea, Department of Semiconductor Engineering, Gachon University, Seongnam, 13120, South Korea; Querrey Simpson Institute for Bioelectronics, Northwestern University, Evanston, 60208, IL, United States, Center for Bio-Integrated Electronics, Northwestern University, Evanston, 60208, IL, United States; Regenerative Neurorehabilitation Laboratory, Shirley Ryan AbilityLab, Chicago, IL, United States; Department of Bioengineering, Samueli School of Engineering, University of California Los Angeles, Los Angeles, 90095, CA, United States; Department of Bioengineering, Samueli School of Engineering, University of California Los Angeles, Los Angeles, 90095, CA, United States, Division of Microsurgery, Department of Orthopedics, E-Da Hospital, I-Shou University Kaohsiung, Kaohsiung, 824005, Taiwan; Querrey Simpson Institute for Bioelectronics, Northwestern University, Evanston, 60208, IL, United States, Division of Plastic Surgery, Feinberg School of Medicine, Northwestern University, Chicago, 60611, IL, United States, Biologics Laboratory, Shirley Ryan AbilityLab, Chicago, 60611, IL, United States; Department of Bioengineering, Samueli School of Engineering, University of California Los Angeles, Los Angeles, 90095, CA, United States, Department of Medicine, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, 90095, CA, United States, Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, Los Angeles, 90095, CA, United States, Jonsson Comprehensive Cancer Center, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, 90095, CA, United States; Querrey Simpson Institute for Bioelectronics, Northwestern University, Evanston, 60208, IL, United States, Department of Mechanical Engineering, Northwestern University, Evanston, 60208, IL, United States, Department of Materials Science and Engineering, Northwestern University, Evanston, 60208, IL, United States, Department of Civil and Environmental Engineering, Northwestern University, Evanston, 60208, IL, United States; Querrey Simpson Institute for Bioelectronics, Northwestern University, Evanston, 60208, IL, United States, Regenerative Neurorehabilitation Laboratory, Shirley Ryan AbilityLab, Chicago, IL, United States, Department of Physical Medicine and Rehabilitation, Northwestern University Feinberg School of Medicine, Chicago, 60611, IL, United States, Ken and Ruth Davee Department of Neurology, Northwestern University Feinberg School of Medicine, Chicago, 60611, IL, United States; Querrey Simpson Institute for Bioelectronics, Northwestern University, Evanston, 60208, IL, United States, Center for Bio-Integrated Electronics, Northwestern University, Evanston, 60208, IL, United States, Department of Mechanical Engineering, Northwestern University, Evanston, 60208, IL, United States, Department of Materials Science and Engineering, Northwestern University, Evanston, 60208, IL, United States, Department of Biomedical Engineering, Northwestern University, Evanston, 60208, IL, United States, Department of Neurological Surgery, Feinberg School of Medicine, Northwestern University, Chicago, 60611, IL, United States | 57715422500; 58140810800; 57125663400; 57189443832; 57213310434; 56036265400; 57476965700; 57189589309; 36727213400; 57199645980; 59870301500; 56683681300; 59907272100; 58778952500; 55728043200; 56300598400; 56992056600; 57190286352; 56594141900; 57226610312; 57391007000; 59715077400; 57216258278; 11739142100; 35229984000; 59758066600; 25958993200; 56755517500 | cfranz@sralab.org; jrogers@northwestern.edu; | Nature Communications | NAT COMMUN | N/A | 2041-1723 | 16 | 1 | SCIE | MULTIDISCIPLINARY SCIENCES | 2024 | 15.7 | 7.0 | 0 | 2025-06-11 | 0 | Absorbable Implants; Action Potentials; Animals; Electric Stimulation; Electric Stimulation Therapy; Male; Mice; Nerve Regeneration; Peripheral Nerve Injuries; Peripheral Nerves; Rats; Rats, Sprague-Dawley; Wireless Technology; anatomy; biostimulation; equipment; injury; instrumentation; muscle; animal cell; animal experiment; animal model; animal tissue; Article; female; histopathology; hydrolysis; in vivo study; male; muscle atrophy; muscle reinnervation; nerve injury; nerve regeneration; nonhuman; peripheral nerve; rat; action potential; animal; biodegradable implant; devices; electrostimulation; electrotherapy; mouse; pathophysiology; peripheral nerve injury; physiology; procedures; Sprague Dawley rat; therapy; wireless communication | English | Final | 2025 | 10.1038/s41467-025-59835-7 | 바로가기 | 바로가기 | 바로가기 |
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