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WoS SCOPUS Document Type Document Title Abstract Authors Affiliation ResearcherID (WoS) AuthorsID (SCOPUS) Author Email(s) Journal Name JCR Abbreviation ISSN eISSN Volume Issue WoS Edition WoS Category JCR Year IF JCR (%) FWCI ⓘ FWCI Update Date WoS Citation SCOPUS Citation Keywords (WoS) KeywordsPlus (WoS) Keywords (SCOPUS) KeywordsPlus (SCOPUS) Language Publication Stage Publication Year Publication Date DOI JCR Link DOI Link WOS Link SCOPUS Link
○ ○ Article ⓘ Energy Transfer-Induced Photoelectrochemical Improvement from Porous Zeolitic Imidazolate Framework-Decorated BiVO4 Photoelectrodes ⓘ BiVO4, which is a representative photoanode material for photoelectrochemical water splitting, intrinsically restricts high conversion efficiency, owing to faster recombination, low electron mobility, and short electron diffusion length. While the photocurrent density of typical BiVO4 corresponds to only 21.3% of the maximum photocurrent density (4.68 mA cm(-2)), decoration of the BiVO4 photoanode with zeolitic imidazolate framework-67 (ZIF-67) exhibits a synergetic effect to raise the overall photocatalytic ability at the BiVO4 surface region to a higher level via the energy-transfer process from BiVO4 to ZIF-67. The hybrid ZIF-67/BiVO4 photoanode follows two convenient photoelectrochemical pathways: 1) energy-transfer-induced water oxidation reaction in ZIF-67 and 2) water oxidation reaction by direct contact between the BiVO4 surface and electrolytes. Compared to the moderate photocurrent density (approximate to 1 mA cm(-2)) of single-layer BiVO4, the proposed ZIF-67/BiVO4 photoanodes show a remarkably high photocurrent (2.25 mA cm(-2)) with high stability, despite the lack of hole scavengers in the electrolyte. Furthermore, the absorbed photon-to-current efficiency of the ZIF-67/BiVO4 photoanode is approximate to 2.5 times greater than that of BiVO4. This work proposes a promising solution for efficient water oxidation that overcomes the intrinsic material limitations of BiVO4 photoelectrodes by using energy transfer-induced photon recycling and the decoration of porous ZIFs. ⓘ Ahn, Cheol Hyoun; Deshpande, Nishad G.; Lee, Ho Seong; Cho, Hyung Koun ⓘ Sungkyunkwan Univ, Sch Adv Mat Sci & Engn, 2066 Seobu Ro, Suwon 16419, Gyeonggi Do, South Korea; Sungkyunkwan Univ, Res Ctr Adv Mat Technol, 2066 Seobu Ro, Suwon 16419, Gyeonggi Do, South Korea; Kyungpook Natl Univ, Sch Mat Sci & Engn, 80 Daehak Ro, Daegu 41566, South Korea ⓘ Deshpande, Nishad/D-1021-2011; Cho, Hyung/P-2007-2017; Cho, Hyung Koun/P-2007-2017 ⓘ 7201986650; 56096311600; 55706810400; 35236454300 ⓘ chohk@skku.edu; ⓘ SMALL METHODS ⓘ SMALL METHODS ⓘ 2366-9608 5 2 SCIE CHEMISTRY, PHYSICAL;MATERIALS SCIENCE, MULTIDISCIPLINARY;NANOSCIENCE & NANOTECHNOLOGY ⓘ 2021 15.367 6.8 1.05 2025-07-30 ⓘ 22 22 bismuth vanadate; energy transfer; photoanodes; photoelectrochemical reactions; zeolitic imidazolate frameworks ⓘ SURFACE RECOMBINATION; CHARGE SEPARATION; MONOCLINIC BIVO4; WATER OXIDATION; ABSORPTION; PHOTOANODE; ZIF-67; COMPOSITE; CATALYSTS ⓘ bismuth vanadate; energy transfer; photoanodes; photoelectrochemical reactions; zeolitic imidazolate frameworks ⓘ Electrochemistry; Electrolytes; Energy transfer; Oxidation; Photocurrents; Photons; Water recycling; Electron diffusion length; Energy transfer process; High conversion efficiency; Photoanode materials; Photocurrent density; Photoelectrochemical water splitting; Photoelectrochemicals; Zeolitic imidazolate frameworks; Bismuth compounds ⓘ English 2021 2021-02 10.1002/smtd.202000753 ⓘ 바로가기 바로가기 바로가기 바로가기
○ ○ Article ⓘ Acute toxicity of the insecticide EPN upon zebrafish (Danio rerio) embryos and its related adverse effects: Verification of abnormal cardiac development and seizure-like events ⓘ Toxicological studies of O-ethyl-O-(4-nitrophenyl) phenylphosphonothioate (EPN) to aquatic vertebrates have been reported, but no reports on toxic mechanism was reported. As zebrafish (Danio rerio) embryos were exposed to EPN, no changes in their survival and hatching rates were observed until 96 h post fertilization (hpf), even at the highest treated concentration of 500 mu g/L. In both 250 mu g/L and 500 mu g/L, edemas were observed in the heart and yolk sac, and a blood pool was also found. Acridine orange staining confirmed apoptotic phynotype, which was the strongest in embryos at 48 hpf. No noticeable difference in the formation and the shape of blood vessels of Tg(fli1a:EGFP) was observed. However, the total body length and number of somite were decreased. Heart formation in Tg(cmlc2:EGFP) were not properly proceeded, and the ventricle did not beat normally at 500 mu g/L level. Cardiac development-related genes, myl7 and nppa, were significantly down- and up-regulated in a concentration-dependent manner. The slowed heartbeat was confirmed using Tg(gata1:EGFP), showing stagnant blood flow and seizure-like events were observed. Altogether, EPN can be the cause for the abnormal heart development accompanied by blood stagnation in embryos, interfering normal development with their inner circulatory system. ⓘ Choe, Hyeseung; Kim, Myoung-Jin; Jeon, Hwang-Ju; Kim, Kyeongnam; Kim, Chaeeun; Park, Jungeun; Shin, Junnyeong; Lee, Sang-Ryong; Lee, Sung-Eun ⓘ Kyungpook Natl Univ, Coll Agr & Life Sci, Dept Appl Biosci, Daegu 41566, South Korea; Kyungpook Natl Univ, Coll Agr & Life Sci, Dept Integrat Biol, Daegu 41566, South Korea; Zefit Inc, 16 Techno Gongwon Ro, Daegu 43017, South Korea; Dongguk Univ, Dept Biol & Environm Sci, Goyang Si 10326, Gyeonggi Do, South Korea ⓘ Jeon, Hwangju/JDW-9035-2023; Lee, Sang-Ryong/C-2167-2017; Kim, Kyeongnam/KSM-2719-2024 ⓘ 57209466586; 57191626729; 56328792200; 57191364349; 57200245142; 58363479900; 57219391155; 57191419414; 55890041600 ⓘ selpest@knu.ac.kr; ⓘ ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY ⓘ ECOTOX ENVIRON SAFE ⓘ 0147-6513 1090-2414 222 SCIE ENVIRONMENTAL SCIENCES;TOXICOLOGY ⓘ 2021 7.129 6.9 0.91 2025-07-30 ⓘ 11 14 Abnormal heart development; EEG measurements; EPN; Heartbeat rate; Stagnant blood flow ⓘ SEPARATION; ENANTIOMERS ⓘ Abnormal heart development; EEG measurements; EPN; Heartbeat rate; Stagnant blood flow ⓘ Animals; Embryo, Nonmammalian; Heart; Insecticides; Seizures; Water Pollutants, Chemical; Zebrafish; Citrus sinensis; Danio rerio; Vertebrata; acridine orange; insecticide; phenylphosphonothioic acid o ethyl o (4 nitrophenyl) ester; protein p53; transcription factor GATA 1; blood; cyprinid; embryonic development; experimental study; physiological response; stagnation flow; survival; toxicity; acute toxicity; angiogenesis; apoptosis; Article; blood flow; cardiovascular system; cause of death; edema; electroencephalogram; fertilization; gene expression; gene mapping; hatchability; hatching; heart development; heart rate; hemostasis; induced pluripotent stem cell; LC50; morphogenesis; mortality rate; nonhuman; seizure; toxicity testing; upregulation; yolk sac; animal; heart; nonmammalian embryo; seizure; toxicity; water pollutant; zebra fish ⓘ English 2021 2021-10-01 10.1016/j.ecoenv.2021.112544 ⓘ 바로가기 바로가기 바로가기 바로가기
○ ○ Article ⓘ Analysis of sticky generative cell mutants reveals that suppression of callose deposition in the generative cell is necessary for generative cell internalization and differentiation in Arabidopsis ⓘ In flowering plants, double fertilization between male and female gametophytes, which are separated by distance, largely depends on the unique pattern of the male gametophyte (pollen): two non-motile sperm cells suspended within a tube-producing vegetative cell. A morphological screen to elucidate the genetic control governing the strategic patterning of pollen has led to the isolation of a sticky generative cell (sgc) mutant that dehisces abnormal pollen with the generative cell immobilized at the pollen wall. Analyses revealed that the sgc mutation is specifically detrimental to pollen development, causing ectopic callose deposition that impedes the timely internalization and differentiation of the generative cell. We found that the SGC gene encodes the highly conserved domain of unknown function 707 (DUF707) gene that is broadly expressed but is germline specific during pollen development. Additionally, transgenic plants co-expressing fluorescently fused SGC protein and known organelle markers showed that SGC localizes in the endoplasmic reticulum, Golgi apparatus and vacuoles in pollen. A yeast two-hybrid screen with an SGC bait identified a thaumatin-like protein that we named GCTLP1, some homologs of which bind and/or digest beta-1,3-glucans, the main constituent of callose. GCTLP1 is expressed in a germline-specific manner and colocalizes with SGC during pollen development, indicating that GCTLP1 is a putative SGC interactor. Collectively, our results show that SGC suppresses callose deposition in the nascent generative cell, thereby allowing the generative cell to fully internalize into the vegetative cell and correctly differentiate as the germline progenitor, with the potential involvement of the GCTLP1 protein, during pollen development in Arabidopsis. ⓘ Oh, Sung-Aeong; Park, Hyo-Jin; Kim, Myung-Hee; Park, Soon-Ki ⓘ Kyungpook Natl Univ, Sch Appl Biosci, Daegu 41566, South Korea ⓘ ⓘ 7404104444; 57195461725; 57221718078; 8055974900 ⓘ psk@knu.ac.kr; ⓘ PLANT JOURNAL ⓘ PLANT J ⓘ 0960-7412 1365-313X 106 1 SCIE PLANT SCIENCES ⓘ 2021 7.091 6.9 0.44 2025-07-30 ⓘ 6 6 sticky generative cell; callose; generative cell internalization; germline differentiation; pollen development; Arabidopsis ⓘ THAUMATIN-LIKE PROTEINS; MALE GAMETOPHYTE DEVELOPMENT; MICROTUBULE ORGANIZATION; GENE FAMILY; MALE GAMETOGENESIS; SIDECAR-POLLEN; MALE-FERTILITY; FUSED KINASE; PLANT; SYNTHASE ⓘ Arabidopsis; callose; generative cell internalization; germline differentiation; pollen development; sticky generative cell ⓘ Callose; Cytology; Deposition; Development; Genes; Pollen; Proteins; Tobacco; Arabidopsis; Arabidopsis Proteins; Cell Differentiation; Gene Expression Regulation, Plant; Glucans; Plants, Genetically Modified; Pollen; Cell proliferation; Deposition; Genes; Plants (botany); Proteins; Tobacco; Arabidopsis protein; callose; glucan; Endoplasmic reticulum; Female gametophytes; Generative cells; Male gametophytes; Pollen development; Thaumatin-like protein; Transgenic plants; Yeast two hybrid; Arabidopsis; cell differentiation; gene expression regulation; genetics; metabolism; physiology; pollen; transgenic plant; Cytology ⓘ English 2021 2021-04 10.1111/tpj.15162 ⓘ 바로가기 바로가기 바로가기 바로가기
○ ○ Article ⓘ Developmental toxicity of 3-phenoxybenzoic acid (3-PBA) and endosulfan sulfate derived from insecticidal active ingredients: Abnormal heart formation by 3-PBA in zebrafish embryos ⓘ Pyrethroid and organochlorine insecticides are enormously used to control agricultural and indoor insect pests. The metabolites of pyrethroid and endosulfan were used to evaluate environmental toxicities using a representative animal model, zebrafish (Danio rerio) embryos in this study. The LC50 values in 3-phenoxy benzoic acid (3-PBA) and endosulfan sulfate (ES) were 1461 mu g/L and 1459 mu g/L, respectively. At the concentration of 2000 mu g/L, spine curvature was observed in the ES-treated embryos. ES showed seizure-like events with an EC50 value of 354 mu g/L. At the concentration of 1000 mu g/L, the pericardial edema was observed in 3-PBA-treated embryos. The inhibition of heart development and the reduction of beating rates were observed in Tg(cmlc2:EGFP) embryos after the exposure to 3-PBA. Down-regulation of the vmhc gene coding ventricular myosin during heart development was significantly found in 3-PBA-treated embryos at 48 hpf, but recovered afterward. It indicates that ventricular malformation occurred at the initial stage of 3-PBA exposure. Considered together, both 3-PBA and ES need public concerns with periodic monitoring of these metabolites in households and agricultural areas to prevent humans and environmental organisms from their unexpected attacks. ⓘ Park, Jungeun; Kim, Chaeeun; Jeon, Hwang-Ju; Kim, Kyeongnam; Kim, Myoung-Jin; Moon, Joon-Kwan; Lee, Sung-Eun ⓘ Kyungpook Natl Univ, Dept Integrat Biol, Daegu 41566, South Korea; Kyungpook Natl Univ, Dept Appl Biosci, Daegu 41566, South Korea; Hankyong Natl Univ, Dept Plant Life & Environm Sci, Ansung 17579, South Korea; Hankyong Natl Univ, Hansalim Agrofood Anal Ctr, Ind Acad Cooperat Fdn, Suwon 16500, South Korea ⓘ Kim, Kyeongnam/KSM-2719-2024; Jeon, Hwangju/JDW-9035-2023 ⓘ 58363479900; 57200245142; 56328792200; 57191364349; 57191626729; 8536720000; 55890041600 ⓘ selpest@knu.ac.kr; ⓘ ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY ⓘ ECOTOX ENVIRON SAFE ⓘ 0147-6513 1090-2414 224 SCIE ENVIRONMENTAL SCIENCES;TOXICOLOGY ⓘ 2021 7.129 6.9 0.84 2025-07-30 ⓘ 15 15 3-phenoxy benzoic acid; Endosulfan sulfate; Heart malformation; Seizure; Transgenic zebrafish ⓘ DANIO-RERIO; METABOLITES; PESTICIDES; EXPOSURE; WATER; ENVIRONMENT; FISH; SOIL ⓘ 3-phenoxy benzoic acid; Endosulfan sulfate; Heart malformation; Seizure; Transgenic zebrafish ⓘ Danio rerio; Hexapoda; (2 methyl [1,1' biphenyl] 3 yl) methanol; 2 (4 chlorophenyl) 3 methylbutanoic acid; 2,2,3,3 tetramethylcyclopropane 1 carboxylic acid; 2,3 dimethyl 3 (2 methylprop 1 en 1 yl) cyclopropane 1 carboxylic acid; 3 (2 chloro 3,3,3 trifluoro1 propenyl) 2,2 dimethylcyclopropane carboxylic acid; 3 (2,2 dibromovinyl) 2,2 dimethylcyclopropane 1 carboxylic acid; 3 (2,2 dichlorovinyl) 2,2 dimethylcyclopropane 1 carboxylic acid; 3 phenoxybenzoic acid; allethrin; bifenthrin; cypermethrin; deltamethrin; endosulfan; endosulfan sulfate; endothelin 1; fenvalerate; myosin heavy chain; pyrethroid; transcription factor GATA 4; transforming growth factor beta2; troponin T; unclassified drug; concentration (composition); cyprinid; dose-response relationship; embryo; endosulfan; environmental impact; inhibition; metabolite; organochlorine pesticide; pyrethroid pesticide; sulfate; toxicity; adult; amhc gene; animal experiment; animal model; Article; congenital heart malformation; controlled study; developmental toxicity; down regulation; EC10; EC50; EC90; edn1 gene; embryo; female; fluorescence microscopy; gene; gene expression; heart development; heart edema; heart rate; LC50; liquid chromatography-mass spectrometry; male; nonhuman; phenotype; proton nuclear magnetic resonance; real time reverse transcription polymerase chain reaction; toxicity testing; ultra performance liquid chromatography; vmhc gene; zebra fish ⓘ English 2021 2021-11 10.1016/j.ecoenv.2021.112689 ⓘ 바로가기 바로가기 바로가기 바로가기
○ ○ Article ⓘ Enhancement of cooling performance of naval combat management system using heat pipe ⓘ Electronic equipment employed in naval combat systems uses various types of printed circuit boards. Because high specification devices are used in such systems, the thermal density of the system is significantly increased. If the system cooling capacity is inadequate in the case of increased thermal loads, the system can malfunction or the system life cycle can decrease. In this study, a heat-pipe-embedded heat sink was newly proposed to improve the conduction heat transfer in heat sinks, and it was directly attached to the heat source mounted on the printed circuit board module. First, an experiment was conducted to measure the thermal resistance of the unit heat pipe. Next, the optimal embedding angle of the heat pipe was determined through experimental and numerical studies using a manufactured prototype of the heat-pipe-embedded heat sink. Finally, computational fluid dynamic analyses of the printed circuit board module adopting the heat-pipe-embedded heat sink were conducted. The proposed method reduced the maximum temperature of the heat source by more than 15 degrees C compared with the original bear heat sink. Additionally, the unit electronic equipment used in the combat management system facilitated 14% space saving, 10% weight reduction, 1% cost saving, and 1% energy saving. ⓘ Song, Jun Gil; Lee, Jong Hui; Park, Il Seouk ⓘ Hanwha Syst, Mech Engn Team, 244,1 Gongdanro, Gumi 39376, Gyeongsangbukdo, South Korea; Kyungpook Natl Univ, Dept Mech Engn, 80 Daehakro, Daegu 41566, South Korea; Kyungpook Natl Univ, Sch Mech Engn, 80 Daehakro, Daegu 41566, South Korea ⓘ Lee, Jong Hui/JFT-0292-2023 ⓘ 57221867042; 57204669495; 50262800000 ⓘ einstein@knu.ac.kr; ⓘ APPLIED THERMAL ENGINEERING ⓘ APPL THERM ENG ⓘ 1359-4311 1873-5606 188 SCIE ENERGY & FUELS;ENGINEERING, MECHANICAL;MECHANICS;THERMODYNAMICS ⓘ 2021 6.465 6.9 0.96 2025-07-30 ⓘ 14 18 Forced convection; Heat pipe; Heat sink; Natural convection; Naval combat system ⓘ ⓘ Forced convection; Heat pipe; Heat sink; Natural convection; Naval combat system ⓘ Computational fluid dynamics; Electronic equipment; Energy conservation; Heat conduction; Heat pipes; Heat sinks; Heating equipment; Life cycle; Printed circuit boards; Combat Management System; Cooling performance; Experimental and numerical studies; Maximum temperature; Naval combat systems; Optimal embedding; System life cycle; Weight reduction; Heat resistance ⓘ English 2021 2021-04 10.1016/j.applthermaleng.2021.116657 ⓘ 바로가기 바로가기 바로가기 바로가기
○ ○ Article ⓘ Fibrinolytic nanocages dissolve clots in the tumor microenvironment, improving the distribution and therapeutic efficacy of anticancer drugs ⓘ Fibrin, one of the components of the extracellular matrix (ECM), acts as a transport barrier within the core of tumors by constricting the blood vessels and forming clots, leading to poor intratumoral distribution of anticancer drugs. Our group previously developed a microplasmin-based thrombolytic ferritin nanocage that efficiently targets and dissolves clots without causing systemic fibrinolysis or disrupting hemostatic clots. We hypothesized that the thrombolytic nanocage-mediated degradation of fibrin clots in the tumor ECM can lead to enhanced intratumoral drug delivery, especially for nanosized anticancer drugs. Fibrin clot deposition worsens after surgery and chemotherapy, further hindering drug delivery. Moreover, the risk of venous thromboembolism (VTE) also increases. Here, we used thrombolytic nanocages with multivalent clot-targeting peptides and fibrin degradation enzymes, such as microplasmin, to dissolve fibrin in the tumor microenvironment and named them fibrinolytic nanocages (FNCs). These FNCs target tumor clots specifically and effectively. FNCs efficiently dissolve fibrin clots inside of the tumor vessels, suggesting that they can mitigate the risk of VTE in cancer patients. Coadministration of FNC and doxorubicin led to improved chemotherapeutic activity in a syngeneic mouse melanoma model. Furthermore, the FNCs increased the distribution of Doxil/doxorubicin nanoparticles within mouse tumors. These results suggest that fibrinolytic cotherapy might help improve the therapeutic efficacy of anticancer nanomedicines. Thus, microplasmin-based fibrinolytic nanocages are promising candidates for this strategy due to their hemostatic safety and ability to home in on the tumor. Cancer: Improving treatment by dissolving clots Engineered "nanocages" that deliver clot-busting enzymes to tumors can improve the potency of chemotherapy and potentially reduce the risk of life-threatening complications. Many tumors form capillary-constricting clots composed of the protein fibrin, which impede drug penetration and create the potential for fatal venous thromboembolism. Researchers led by Soyoun Kim of Kyungpook National University, Daegu, South Korea, have devised a promising intervention that uses fibrin-degrading enzymes to clear these clogged vessels. The enzyme is delivered in nanocages composed of the protein ferritin coupled to short peptides that selectively bind fibrin, essentially targeting the treatment to clots. When they administered these nanocages to a mouse model of melanoma, the researchers observed a significantly improved response to chemotherapy and greater tumor penetration. This selective fibrin degradation should also eliminate the conditions that predispose patients to thrombembolism. ⓘ Seo, Junyoung; Do Yoo, Jae; Kim, Minseong; Shim, Gayong; Oh, Yu-Kyoung; Park, Rang-Woon; Lee, Byungheon; Kim, In-San; Kim, Soyoun ⓘ Kyungpook Natl Univ, Sch Med, Cell & Matrix Res Inst, Dept Biochem & Cell Biol, Daegu 41944, South Korea; Kyungpook Natl Univ, Dept Biomed Sci, Sch Med, B21 Plus KNU Biomed Convergence Program, 680 Gukchaebosang Ro, Daegu 41944, South Korea; Soongsil Univ, Sch Syst Biomed Sci, Seoul 06978, South Korea; Seoul Natl Univ, Coll Pharm, Seoul 08826, South Korea; Korea Inst Sci & Technol, Biomed Res Inst, Seoul 02792, South Korea; Korea Univ, KU KIST Sch, Seoul 02841, South Korea ⓘ Shim, Gayong/AAU-5782-2020 ⓘ 56573206100; 57209981694; 57221718142; 23101400500; 7402125977; 7401895636; 16304374900; 34770432800; 58847992000 ⓘ soyounki@knu.ac.kr; ⓘ EXPERIMENTAL AND MOLECULAR MEDICINE ⓘ EXP MOL MED ⓘ 1226-3613 2092-6413 53 10 SCIE BIOCHEMISTRY & MOLECULAR BIOLOGY;MEDICINE, RESEARCH & EXPERIMENTAL ⓘ 2021 12.172 6.9 0.96 2025-07-30 ⓘ 16 18 ⓘ SOLID TUMORS; COMBINATION CHEMOTHERAPY; CANCER; MICROPLASMIN; PENETRATION; RESISTANCE; FIBRINOGEN; DELIVERY; TISSUE; THROMBOEMBOLISM ⓘ ⓘ Animals; Antineoplastic Agents; Fibrinolysis; Humans; Mice; Thrombolytic Therapy; Thrombosis; Tumor Microenvironment; doxorubicin; fibrin; fibrinolytic nanocage; nanocage; ocriplasmin; unclassified drug; antineoplastic agent; animal cell; animal experiment; animal model; animal tissue; Article; cancer cell culture; cancer tissue; controlled study; drug accumulation; drug delivery system; drug distribution; drug efficacy; extracellular matrix; female; fibrin clot; fibrinolysis; glioblastoma cell line; human; human cell; in vitro study; in vivo study; male; melanoma; melanoma cell line; mouse; nanomedicine; nonhuman; tumor microenvironment; venous thromboembolism; animal; fibrinolytic therapy; metabolism; procedures; thrombosis; tumor microenvironment ⓘ English 2021 2021-10 10.1038/s12276-021-00688-7 ⓘ 바로가기 바로가기 바로가기 바로가기
○ ○ Article ⓘ Fine-mapping and candidate gene analysis for the foxglove aphid resistance gene Raso2 from wild soybean PI 366121 ⓘ Key message The foxglove aphid resistance geneRaso2from PI 366121 was fine-mapped to 77 Kb region, and one candidate gene was identified. The foxglove aphid (FA: Aulacorthum solani Kaltenbach) is an important insect pest that causes serious yield losses in soybean. The FA resistance gene Raso2 from wild soybean PI 366121 was previously mapped to a 13 cM interval on soybean chromosome 7. However, fine-mapping of Raso2 was needed to improve the effectiveness of marker-assisted selection (MAS) and to eventually clone it. The objectives of this study were to fine-map Raso2 from PI 366121 using Axiom(R) 180 K SoyaSNP array, to confirm the resistance and inheritance of Raso2 in a different background, and to identify candidate gene(s). The 105 F-4:8 recombinant inbred lines were used to fine-map the gene and to test antibiosis and antixenosis of Raso2 to FA. These efforts resulted in the mapping of Raso2 on 1 cM interval which corresponds to 77 Kb containing eight annotated genes based on the Williams 82 reference genome assembly (Wm82.a2.v1). Interestingly, all nonsynonymous substitutions were in Glyma.07g077700 which encodes the disease resistance protein containing LRR domain and expression of the gene in PI 366121 was significantly higher than that in Williams 82. In addition, distinct SNPs within Glyma.07g077700 that can distinguish PI 366121 and diverse FA-susceptible soybeans were identified. We also confirmed that Raso2 presented the resistance to FA and the Mendelian inheritance for single dominant gene in a different background. The results of this study would provide fundamental information on MAS for development of FA-resistant cultivars as well as functional study and cloning of the candidate gene in soybean. ⓘ Kim, Ki-Seung; Kim, Ji-Min; Jung, Jiyeong; Shin, Ilseob; Park, Sumin; Lee, Ju Seok; Jeong, Soon-Chun; Lee, Jeong-Dong; Jung, Jin Kyo; Ha, Bo-Keun; Kang, Sungtaeg ⓘ FarmHannong Ltd, Deparment Innovat Technol, Nonsan 33010, South Korea; Dankook Univ, Dept Crop Sci & Biotechnol, Cheonan 31116, South Korea; Korea Res Inst Biosci & Biotechnol, Bioevaluat Ctr, Cheongju 28116, South Korea; Kyungpook Natl Univ, Sch Appl Biosci, Daegu 41566, South Korea; Natl Inst Crop Sci, Rural Dev Adm, Suwon 16613, South Korea; Chonnam Natl Univ, Dept Appl Plant Sci, Gwangju 61186, South Korea; Business Incubation Ctr, Sae Han Agr Technol Res Stn, Hwaseong 18330, South Korea ⓘ ⓘ 24463202100; 57211517085; 57219265899; 57223439186; 57223432881; 57871955400; 55646368100; 40462075100; 57191266304; 7006749575; 9736708500 ⓘ kangst@dankook.ac.kr; ⓘ THEORETICAL AND APPLIED GENETICS ⓘ THEOR APPL GENET ⓘ 0040-5752 1432-2242 134 8 SCIE AGRONOMY;GENETICS & HEREDITY;HORTICULTURE;PLANT SCIENCES ⓘ 2021 5.574 6.9 0.62 2025-07-30 ⓘ 8 9 ⓘ SPODOPTERA-LITURA FABRICIUS; QTL; IDENTIFICATION; ANTIXENOSIS; ANTIBIOSIS ⓘ ⓘ Animals; Aphids; Chromosome Mapping; Chromosomes, Plant; Disease Resistance; Gene Expression Regulation, Plant; Phenotype; Plant Diseases; Plant Proteins; Polymorphism, Single Nucleotide; Soybeans; Clone cells; Cloning; Mapping; Plants (botany); plant protein; Annotated genes; Candidate genes; Disease resistance; Genome assembly; Marker-assisted selection; Recombinant inbred lines; Resistance genes; Resistant cultivars; animal; aphid; chromosomal mapping; disease resistance; gene expression regulation; genetics; growth, development and aging; immunology; metabolism; parasitology; phenotype; physiology; plant chromosome; plant disease; procedures; single nucleotide polymorphism; soybean; Genes ⓘ English 2021 2021-08 10.1007/s00122-021-03853-8 ⓘ 바로가기 바로가기 바로가기 바로가기
○ ○ Article ⓘ GORI, encoding the WD40 domain protein, is required for pollen tube germination and elongation in rice ⓘ Successful delivery of sperm cells to the embryo sac in higher plants is mediated by pollen tube growth. The molecular mechanisms underlying pollen germination and tube growth in crop plants remain rather unclear, although these mechanisms are crucial to plant reproduction and seed formation. By screening pollen-specific gene mutants in rice (Oryza sativa), we identified a T-DNA insertional mutant of Germinating modulator of rice pollen (GORI) that showed a one-to-one segregation ratio for wild type (WT) to heterozygous. GORI encodes a seven-WD40-motif protein that is homologous to JINGUBANG/REN4 in Arabidopsis. GORI is specifically expressed in rice pollen, and its protein is localized in the nucleus, cytosol and plasma membrane. Furthermore, a homozygous mutant, gori-2, created through CRISPR-Cas9 clearly exhibited male sterility with disruption of pollen tube germination and elongation. The germinated pollen tube of gori-2 exhibited decreased actin filaments and altered pectin distribution. Transcriptome analysis revealed that 852 pollen-specific genes were downregulated in gori-2 compared with the WT, and Gene Ontology enrichment analysis indicated that these genes are strongly associated with cell wall modification and clathrin coat assembly. Based on the molecular features of GORI, phenotypical observation of the gori mutant and its interaction with endocytic proteins and Rac GTPase, we propose that GORI plays key roles in forming endo-/exocytosis complexes that could mediate pollen tube growth in rice. ⓘ Kim, Yu-Jin; Kim, Myung-Hee; Hong, Woo-Jong; Moon, Sunok; Kim, Eui-Jung; Silva, Jeniffer; Lee, Jinwon; Lee, Sangho; Kim, Sun Tae; Park, Soon Ki; Jung, Ki-Hong ⓘ Pusan Natl Univ, Dept Life Sci & Environm Biochem, Miryang 50463, South Korea; Kyungpook Natl Univ, Sch Appl Biosci, Daegu 41566, South Korea; Kyung Hee Univ, Grad Sch Biotechnol, Yongin 17104, South Korea; Kyung Hee Univ, Crop Biotech Inst, Yongin 17104, South Korea; Sungkyunkwan Univ, Dept Biol Sci, Suwon, South Korea; Pusan Natl Univ, Dept Plant Biosci, Miryang 50463, South Korea ⓘ Kim, Yu/L-8480-2017; Hong, Woo-Jong/HPF-0756-2023; Jung, Ki/L-5570-2019 ⓘ 57074286200; 57221718078; 57194418669; 7401616474; 57214231436; 56780864200; 57210899223; 56177341300; 35484493400; 8055974900; 56022522000 ⓘ psk@knu.ac.kr;khjung2010@khu.ac.kr; ⓘ PLANT JOURNAL ⓘ PLANT J ⓘ 0960-7412 1365-313X 105 6 SCIE PLANT SCIENCES ⓘ 2021 7.091 6.9 2.91 2025-07-30 ⓘ 49 46 Oryza sativa; GORI; pollen tube growth; CRISPR-Cas9; RNA-seq analysis; WD40 protein; Brefeldin A; endocytosis ⓘ BREFELDIN-A; AUXIN TRANSPORT; TIP GROWTH; DYNAMICS; PLANTS; ACCUMULATION; ENDOCYTOSIS; EXPRESSION; BINDING; REPEAT ⓘ Brefeldin A; CRISPR-Cas9; endocytosis; GORI; Oryza sativa; pollen tube growth; RNA-seq analysis; WD40 protein ⓘ Cultivation; Farm Crops; Genes; Pollen; Proteins; Rice; Tubes; Gene Expression Regulation, Plant; Germination; Oryza; Plant Proteins; Pollen Tube; RNA-Seq; Cell membranes; Cell proliferation; Crops; Cultivation; Encoding (symbols); Genes; Proteins; Seed; Signal encoding; Tubes (components); plant protein; Endocytic proteins; Molecular mechanism; Plant reproduction; Pollen germination; Pollen tube growth; Pollen-specific genes; Rice (oryza sativa); Transcriptome analysis; gene expression regulation; genetics; germination; metabolism; Oryza; physiology; pollen tube; Plants (botany) ⓘ English 2021 2021-03 10.1111/tpj.15139 ⓘ 바로가기 바로가기 바로가기 바로가기
○ ○ Article ⓘ Metabolic activities affect femur and lumbar vertebrae remodeling, and anti-resorptive risedronate disturbs femoral cortical bone remodeling ⓘ Metabolic activities are closely correlated with bone remodeling and long-term anti-resorptive bisphosphonate treatment frequently causes atypical femoral fractures through unclear mechanisms. To explore whether metabolic alterations affect bone remodeling in femurs and lumbar vertebrae and whether anti-osteoporotic bisphosphonates perturb their reconstruction, we studied three mouse strains with different fat and lean body masses (BALB/c, C57BL6, and C3H mice). These mice displayed variable physical activity, food and drink intake, energy expenditure, and respiratory quotients. Following intraperitoneal calcein injection, double calcein labeling of the femoral diaphysis, as well as serum levels of the bone-formation marker procollagen type-I N-terminal propeptide and the bone-resorption marker C-terminal telopeptide of type-I collagen, revealed increased bone turnover in mice in the following order: C3H > BALB/c >= C57BL6 mice. In addition, bone reconstitution in femurs was distinct from that in lumbar vertebrae in both healthy control and estrogen-deficient osteoporotic mice with metabolic perturbation, particularly in terms of femoral trabecular and cortical bone remodeling in CH3 mice. Interestingly, subcutaneous administration of bisphosphonate risedronate to C3H mice with normal femoral bone density led to enlarged femoral cortical bones with a low bone mineral density, resulting in bone fragility; however, this phenomenon was not observed in mice with ovariectomy-induced femoral cortical bone loss. Together, these results suggest that diverse metabolic activities support various forms of bone remodeling and that femur remodeling differs from lumbar vertebra remodeling. Moreover, our findings imply that the adverse effect of bisphosphonate agents on femoral cortical bone remodeling should be considered when prescribing them to osteoporotic patients. ⓘ Kim, Mi Yeong; Lee, Kyunghee; Shin, Hong-In; Lee, Kyung-Jae; Jeong, Daewon ⓘ Yeungnam Univ, Dept Microbiol, Lab Bone Metab & Control, Coll Med, Daegu 42415, South Korea; Kyungpook Natl Univ, Sch Dent, Dept Oral Pathol, IHBR, Daegu 41940, South Korea; Keimyung Univ, Sch Med, Dept Orthopaed Surg, Dongsan Hosp, Daegu 42601, South Korea ⓘ Lee, Jae-Hyun/ABE-3803-2020 ⓘ 57190961069; 57196250274; 24377179200; 23992675200; 55548865300 ⓘ oslee@dsmc.or.kr;dwjeong@ynu.ac.kr; ⓘ EXPERIMENTAL AND MOLECULAR MEDICINE ⓘ EXP MOL MED ⓘ 1226-3613 2092-6413 53 1 SCIE BIOCHEMISTRY & MOLECULAR BIOLOGY;MEDICINE, RESEARCH & EXPERIMENTAL ⓘ 2021 12.172 6.9 0.56 2025-07-30 ⓘ 8 8 ⓘ ENERGY-METABOLISM; MINERAL DENSITY; CALORIC RESTRICTION; INBRED STRAINS; FRACTURE RISK; OLDER WOMEN; DISCORDANCE; HIP; SPINE; PREVALENCE ⓘ ⓘ Animals; Biomechanical Phenomena; Bone Density Conservation Agents; Bone Remodeling; Collagen Type I; Cortical Bone; Estrogens; Femur; Fluoresceins; Lumbar Vertebrae; Mice; Mice, Inbred BALB C; Mice, Inbred C3H; Mice, Inbred C57BL; Osteoporosis; Risedronic Acid; amino terminal telopeptide; bisphosphonic acid derivative; calcein; carboxy terminal telopeptide; risedronic acid; bone density conservation agent; collagen type 1; estrogen; fluorescein derivative; risedronic acid; adverse event; animal experiment; animal model; animal tissue; antiosteoporotic activity; Article; bone density; bone fragility; bone remodeling; bone turnover; controlled study; cortical bone; energy expenditure; fat mass; female; femur; femur diaphysis; fluid intake; food intake; lean body weight; lumbar vertebra; metabolic activation; mouse; nonhuman; ovariectomy-induced osteoporosis; physical activity; respiratory quotient; trabecular bone; animal; Bagg albino mouse; biomechanics; C3H mouse; C57BL mouse; cortical bone; drug effect; femur; genetics; lumbar vertebra; metabolism; osteoporosis; physiology ⓘ English 2021 2021-01 10.1038/s12276-020-00548-w ⓘ 바로가기 바로가기 바로가기 바로가기
○ ○ Article ⓘ Multiplexed targeting of miRNA-210 in stem cell-derived extracellular vesicles promotes selective regeneration in ischemic hearts ⓘ Heart disease: Boosting microRNA activity may restore heart function A therapy designed to enhance the activity of a small regulatory RNA molecule could prompt the restoration of cellular communication and improve heart function during heart disease. Heart cells struggle to function and regenerate under the reduced oxygen conditions caused by narrowed or blocked blood vessels. Stem cell-derived therapies may restore damaged heart tissues, and can be designed to secrete specific factors that boost regeneration. To identify factors that may improve heart recovery chances, Woochul Chang at Pusan National University, Busan, Korea and co-workers examined stem cell-derived extracellular vesicles (EVs), cellular membrane sacs that carry proteins and RNAs to surrounding tissues and boost intercellular communication. The team identified microRNA-210 as a key factor that inhibits heart cell death, reduces fibrosis, and enhances regeneration of heart cell populations. EVs secreting microRNA-210 may prove a useful therapeutic tool. Extracellular vesicles (EVs) are cell derivatives containing diverse cellular molecules, have various physiological properties and are also present in stem cells used for regenerative therapy. We selected a "multiplexed target" that demonstrates multiple effects on various cardiovascular cells, while functioning as a cargo of EVs. We screened various microRNAs (miRs) and identified miR-210 as a candidate target for survival and angiogenic function. We confirmed the cellular and biological functions of EV-210 (EVs derived from ASC(miR-210)) secreted from adipose-derived stem cells (ASCs) transfected with miR-210 (ASC(miR-210)). Under hypoxic conditions, we observed that ASC(miR-210) inhibits apoptosis by modulating protein tyrosine phosphatase 1B (PTP1B) and death-associated protein kinase 1 (DAPK1). In hypoxic endothelial cells, EV-210 exerted its angiogenic capacity by inhibiting Ephrin A (EFNA3). Furthermore, EV-210 enhanced cell survival under the control of PTP1B and induced antiapoptotic effects in hypoxic H9c2 cells. In cardiac fibroblasts, the fibrotic ratio was reduced after exposure to EV-210, but EVs derived from ASC(miR-210) did not communicate with fibroblasts. Finally, we observed the functional restoration of the ischemia/reperfusion-injured heart by maintaining the intercommunication of EVs and cardiovascular cells derived from ASC(miR-210). These results suggest that the multiplexed target with ASC(miR-210) is a useful tool for cardiovascular regeneration. ⓘ Song, Byeong-Wook; Lee, Chang Youn; Kim, Ran; Kim, Won Jung; Lee, Hee Won; Lee, Min Young; Kim, Jongmin; Jeong, Jee-Yeong; Chang, Woochul ⓘ Catholic Kwandong Univ, Inst Biomed Convergence, Int St Marys Hosp, Incheon, South Korea; Yonsei Univ, Grad Sch, Dept Integrated Omics Biomed Sci, Seoul, South Korea; Pusan Natl Univ, Coll Educ, Dept Biol Educ, Busan, South Korea; Kyungpook Natl Univ, Coll Pharm, Dept Mol Physiol, Daegu, South Korea; Sookmyung Womens Univ, Dept Life Syst, Seoul, South Korea; Kosin Univ, Dept Biochem, Coll Med, Busan, South Korea ⓘ ; Lee, Chang Youn/JUF-8732-2023; Song, Byeong-Wook/R-1077-2019; Kim, Jinsoo/G-6348-2012 ⓘ 24345452200; 57193241793; 55859511600; 57204267974; 57204267330; 15119890400; 57007739600; 23034989800; 12797539700 ⓘ wchang1975@pusan.ac.kr; ⓘ EXPERIMENTAL AND MOLECULAR MEDICINE ⓘ EXP MOL MED ⓘ 1226-3613 2092-6413 53 4 SCIE BIOCHEMISTRY & MOLECULAR BIOLOGY;MEDICINE, RESEARCH & EXPERIMENTAL ⓘ 2021 12.172 6.9 1.52 2025-07-30 ⓘ 23 22 ⓘ TISSUE GROWTH-FACTOR; THERAPEUTIC TARGETS; EXOSOMES; HYPOXIA; MICRORNAS; MECHANISMS; EXPRESSION; APOPTOSIS; FIBROSIS; BETA ⓘ ⓘ Animals; Cells, Cultured; Extracellular Vesicles; Human Umbilical Vein Endothelial Cells; Humans; Hypoxia; Male; Mesenchymal Stem Cells; MicroRNAs; Models, Biological; Myocardial Ischemia; Myocytes, Cardiac; Rats; Regeneration; Stem Cells; Transfection; death associated protein kinase; ephrin A3; microRNA 210; protein tyrosine phosphatase 1B; microRNA; MIRN210 microRNA, human; adipose derived stem cell; animal experiment; animal model; apoptosis; Article; cell communication; cell function; cell regeneration; cell survival; cellular secretion; controlled study; endothelium cell; exosome; gene function; gene targeting; H9c2(2-1) cell line; heart fibroblast; heart function; heart muscle ischemia; human; human cell; in vitro study; in vivo study; male; nonhuman; reperfusion injury; stem cell; animal; biological model; cardiac muscle cell; cell culture; cytology; exosome; genetic transfection; genetics; heart muscle ischemia; hypoxia; mesenchymal stem cell; metabolism; rat; regeneration; stem cell; umbilical vein endothelial cell ⓘ English 2021 2021-04 10.1038/s12276-021-00584-0 ⓘ 바로가기 바로가기 바로가기 바로가기
○ ○ Article ⓘ OsMTD2-mediated reactive oxygen species (ROS) balance is essential for intact pollen-tube elongation in rice ⓘ The highly specialized haploid male gametophyte-pollen consist of two sperm cells and a large vegetative cell. Successful fertilization requires proper growth timing and rupture of the pollen tube until it delivers sperm cells, which occur immediately after a pollen grain hydrates. Although a tight regulation on polar cell-wall expansion of the pollen tube is fundamentally important, the underlying molecular mechanism remains largely unknown, especially in crop plants. Here, we characterized the function of male-gene transfer defective 2 (OsMTD2) gene in rice (Oryza sativa), which belongs to the plant-specific receptor-like kinase, the CrRLK1L family. We demonstrated that OsMTD2 is an essential male factor participating in pollen-tube elongation based on genetic evidence and physiological observations. Because of unavailability of homozygous mutant via conventional methods, we used CRISPR-Cas9 system to obtain homozygous knockout mutant of OsMTD2. We were able to identify phenotypic changes including male sterility due to early pollen-tube rupture in the mutant. We observed that the production of reactive oxygen species (ROS) was dramatically reduced in mutants of OsMTD2 pollen grain and tubes with defective pectin distribution. Transcriptome analysis of osmtd2-2 versus wild-type anthers revealed that genes involved in defense responses, metabolic alteration, transcriptional and protein modification were highly upregulated in the osmtd2-2 mutant. Through yeast-two-hybrid screening, we found that OsMTD2 kinase interacts with E3 ligase SPL11. Taken together, we propose that OsMTD2 has crucial functions in promoting pollen-tube elongation through cell-wall modification, possibly by modulating ROS homeostasis during pollen-tube growth. ⓘ Kim, Yu-Jin; Kim, Myung-Hee; Hong, Woo-Jong; Moon, Sunok; Kim, Sun Tae; Park, Soon Ki; Jung, Ki-Hong ⓘ Pusan Natl Univ, Dept Life Sci & Environm Biochem, Miryang, South Korea; Pusan Natl Univ, Life & Ind Convergence Res Inst, Miryang, South Korea; Kyungpook Natl Univ, Sch Appl Biosci, Daegu, South Korea; Kyung Hee Univ, Grad Sch Biotechnol, Yongin, South Korea; Kyung Hee Univ, Crop Biotech Inst, Yongin, South Korea; Pusan Natl Univ, Dept Plant Biosci, Miryang, South Korea ⓘ Hong, Woo-Jong/HPF-0756-2023; Kim, Yu/L-8480-2017; Jung, Ki/L-5570-2019 ⓘ 57074286200; 57221718078; 57194418669; 7401616474; 57225142626; 8055974900; 56022522000 ⓘ psk@knu.ac.kr;khjung2010@khu.ac.kr; ⓘ PLANT JOURNAL ⓘ PLANT J ⓘ 0960-7412 1365-313X 107 4 SCIE PLANT SCIENCES ⓘ 2021 7.091 6.9 1.96 2025-07-30 ⓘ 33 32 pollen-tube elongation; OsMTD2; CrRLK1L; CRISPR; Cas9; reactive oxygen species; cell-wall expansion; rice ⓘ RECEPTOR-LIKE KINASES; CELL-WALL INTEGRITY; NADPH OXIDASE; GROWTH; GENERATION; REGULATOR; PATHWAY; PLANTS; GENES; DEATH ⓘ cell-wall expansion; CRISPR/Cas9; CrRLK1L; OsMTD2; pollen-tube elongation; reactive oxygen species; rice ⓘ Cytology; Defects; Elongation; Enzymes; Farm Crops; Genes; Cell Membrane; Gene Expression Regulation, Plant; Germination; Mutation; Oryza; Plant Proteins; Plants, Genetically Modified; Pollen; Pollen Tube; Protein Processing, Post-Translational; Reactive Oxygen Species; Two-Hybrid System Techniques; Crops; Cytology; Defects; Elongation; Enzymes; Gene transfer; Genes; Grain (agricultural product); Oxygen; Reactive oxygen species; Tubes (components); plant protein; reactive oxygen metabolite; Conventional methods; Molecular mechanism; Physiological observation; Pollen tube growth; Protein modifications; Receptor-like kinase; Rice (oryza sativa); Transcriptome analysis; cell membrane; gene expression regulation; genetics; germination; metabolism; mutation; Oryza; physiology; pollen; pollen tube; protein processing; transgenic plant; two hybrid system; Plants (botany) ⓘ English 2021 2021-08 10.1111/tpj.15373 ⓘ 바로가기 바로가기 바로가기 바로가기
○ ○ Article ⓘ Pyruvate dehydrogenase kinase 1 and 2 deficiency reduces high-fat diet-induced hypertrophic obesity and inhibits the differentiation of preadipocytes into mature adipocytes ⓘ Obesity is now recognized as a disease. This study revealed a novel role for pyruvate dehydrogenase kinase (PDK) in diet-induced hypertrophic obesity. Mice with global or adipose tissue-specific PDK2 deficiency were protected against diet-induced obesity. The weight of adipose tissues and the size of adipocytes were reduced. Adipocyte-specific PDK2 deficiency slightly increased insulin sensitivity in HFD-fed mice. In studies with 3T3-L1 preadipocytes, PDK2 and PDK1 expression was strongly increased during adipogenesis. Evidence was found for epigenetic induction of both PDK1 and PDK2. Gain- and loss-of-function studies with 3T3-L1 cells revealed a critical role for PDK1/2 in adipocyte differentiation and lipid accumulation. PDK1/2 induction during differentiation was also accompanied by increased expression of hypoxia-inducible factor-1 alpha (HIF1 alpha) and enhanced lactate production, both of which were absent in the context of PDK1/2 deficiency. Exogenous lactate supplementation increased the stability of HIF1 alpha and promoted adipogenesis. PDK1/2 overexpression-mediated adipogenesis was abolished by HIF1 alpha inhibition, suggesting a role for the PDK-lactate-HIF1 alpha axis during adipogenesis. In human adipose tissue, the expression of PDK1/2 was positively correlated with that of the adipogenic marker PPAR gamma and inversely correlated with obesity. Similarly, PDK1/2 expression in mouse adipose tissue was decreased by chronic high-fat diet feeding. We conclude that PDK1 and 2 are novel regulators of adipogenesis that play critical roles in obesity. Obesity: Effects of different forms of key enzyme The discovery that two forms of a key enzyme appear to play a critical role in fat production triggered by overeating might lead to new approaches to prevent and treat obesity. Hyeon-Ji Kang at Kyungpook National University, Daegu, South Korea, and colleagues in South Korea and the USA examined the role of the enzymes pyruvate dehydrogenase kinase types 1 and 2 (PDK1/2). PDK enzymes regulate the activity of a multi-enzyme complex that catalyzes a key step in the use of glucose to provide energy stores for cells. Mice deficient in PDK2 were protected from diet-induced obesity, and PDK 1 and 2 activity was increased during the generation of fat cells. Studies using mice and human fat tissue confirmed that the enzymes regulate the development and growth of fat cells. Drugs inhibiting PDK enzymes might combat obesity. ⓘ Kang, Hyeon-Ji; Min, Byong-Keol; Choi, Won-Il; Jeon, Jae-Han; Kim, Dong Wook; Park, Sungmi; Lee, Yun-Kyung; Kim, Hwa-jin; Byeon, Ju-Eun; Go, Younghoon; Ham, Hye Jin; Jeon, Yong Hyun; Kim, Mi-Jin; Lee, Jung Yi; Wende, Adam R.; Choi, Sung Hee; Harris, Robert A.; Lee, In-Kyu ⓘ Kyungpook Natl Univ, Res Inst Aging & Metab, Daegu, South Korea; Arbormed Co Ltd, Seoul, South Korea; Korea Adv Inst Sci & Technol, Grad Sch Med Sci & Engn, Daejeon, South Korea; Kyungpook Natl Univ Hosp, Leading Edge Res Ctr Drug Discovery & Dev Diabet, Daegu, South Korea; Kyungpook Natl Univ, Chilgok Hosp, Sch Med, Dept Internal Med, Daegu, South Korea; Seoul Natl Univ, Dept Biochem, Coll Vet Med, Seoul, South Korea; Seoul Natl Univ, Dept Internal Med, Bundang Hosp, Seongnam, South Korea; Kyungpook Natl Univ, Dept Biomed Sci, BK21 Plus KNU Biomed Convergence Programs, Daegu, South Korea; Kyungpook Natl Univ, Grad Sch, Dept Biomed Sci, Daegu, South Korea; Korea Inst Oriental Med, Korean Med Applicat Ctr, Daegu, South Korea; Daegu Gyeongbuk Med Innovat Fdn, Lab Anim Ctr, Daegu, South Korea; Univ Alabama Birmingham, Dept Pathol, Div Mol & Cellular Pathol, Birmingham, AL 35294 USA; Univ Kansas, Med Ctr, Dept Biochem & Mol Biol, Kansas City, KS 66160 USA; Kyungpook Natl Univ, Kyungpook Natl Univ Hosp, Sch Med, Dept Internal Med, Daegu, South Korea ⓘ ; Jeon, Yong/N-6910-2019; Choi, Sung-hee/J-5689-2012; Wende, Adam/AFG-8878-2022; Lee, In-Kyu/AAR-6374-2021; CHOI, WON/J-1665-2012; Lee, Yun Kyung/AFE-1810-2022 ⓘ 55946300800; 56650523400; 57191264924; 36910340400; 57195202065; 56252420000; 57190032136; 57201359011; 57266369700; 55455882000; 57191361390; 16042453400; 56984392800; 57195563161; 6602484414; 56181488400; 58710709100; 36071537600 ⓘ drshchoi@snu.ac.kr;raharris@iu.edu;leei@knu.ac.kr; ⓘ EXPERIMENTAL AND MOLECULAR MEDICINE ⓘ EXP MOL MED ⓘ 1226-3613 2092-6413 53 9 SCIE BIOCHEMISTRY & MOLECULAR BIOLOGY;MEDICINE, RESEARCH & EXPERIMENTAL ⓘ 2021 12.172 6.9 0.64 2025-07-30 ⓘ 9 9 ⓘ ADIPOSE-TISSUE; RNA ACCUMULATION; PPAR-GAMMA; TRANSCRIPTION; INSULIN; MICE; EXPRESSION; ENHANCERS; CELLS ⓘ ⓘ 3T3-L1 Cells; Adipocytes; Adipogenesis; Adiposity; Animals; Biomarkers; Cell Differentiation; Diet, High-Fat; Gene Expression; Glycolysis; Insulin Resistance; Lactic Acid; Mice; Mice, Knockout; Obesity; Organ Size; Pyruvate Dehydrogenase Acetyl-Transferring Kinase; hypoxia inducible factor 1alpha; lactic acid; peroxisome proliferator activated receptor gamma; pyruvate dehydrogenase kinase; pyruvate dehydrogenase kinase 1; pyruvate dehydrogenase kinase 2; unclassified drug; biological marker; lactic acid; Pdk1 protein, mouse; Pdk2 protein, mouse; 3T3-L1 cell line; adipocyte; adipogenesis; animal cell; animal experiment; animal model; Article; body mass; C57BL 6 mouse; cell differentiation; controlled study; diet-induced obesity; epigenetics; fat mass; gain of function mutation; insulin sensitivity; lipid storage; loss of function mutation; male; mouse; nonhuman; proadipocyte; protein expression; adipocyte; adverse event; animal; cell differentiation; cytology; gene expression; genetics; glycolysis; insulin resistance; knockout mouse; lipid diet; metabolism; obesity; organ size; pathology ⓘ English 2021 2021-09 10.1038/s12276-021-00672-1 ⓘ 바로가기 바로가기 바로가기 바로가기
○ ○ Article ⓘ Regulation of MDM2 E3 ligase-dependent vascular calcification by MSX1/2 ⓘ Vascular disease: Driver of deposition of calcium salts identified The identification of a signaling pathway involved in triggering vascular calcification, the deposition of calcium phosphate crystals in blood vessels, could inform new therapeutic interventions for related cardiovascular complications. Vascular calcification causes significant complications in patients with metabolic syndrome, renal failure, or cardiovascular disease. In their previous work, Hyun Kook and Duk-Hwa Kwon at Chonnam National University Medical School, Jeollanamdo, Republic of Korea, and coworkers demonstrated that the E3 ligase activity of a protein called MDM2 induces calcification. Now, following further mouse trials, the team have identified an upstream signaling pathway involving several development proteins such as MSX1 and MSX2 which activate MDM2. The activation of this signaling axis leads to the degradation of a key protein that would otherwise prevent calcification. The results may provide a platform for novel therapies targeting the condition. Vascular calcification increases morbidity and mortality in patients with cardiovascular and renal diseases. Previously, we reported that histone deacetylase 1 prevents vascular calcification, whereas its E3 ligase, mouse double minute 2 homolog (MDM2), induces vascular calcification. In the present study, we identified the upstream regulator of MDM2. By utilizing cellular models and transgenic mice, we confirmed that E3 ligase activity is required for vascular calcification. By promoter analysis, we found that both msh homeobox 1 (Msx1) and msh homeobox 2 (Msx2) bound to the MDM2 promoter region, which resulted in transcriptional activation of MDM2. The expression levels of both Msx1 and Msx2 were increased in mouse models of vascular calcification and in calcified human coronary arteries. Msx1 and Msx2 potentiated vascular calcification in cellular and mouse models in an MDM2-dependent manner. Our results establish a novel role for MSX1/MSX2 in the transcriptional activation of MDM2 and the resultant increase in MDM2 E3 ligase activity during vascular calcification. ⓘ Kwon, Duk-Hwa; Choe, Nakwon; Shin, Sera; Ryu, Juhee; Kim, Nacksung; Eom, Gwang Hyeon; Nam, Kwang-Il; Kim, Hyung Seok; Ahn, Youngkeun; Kim, Young-Kook; Park, Woo Jin; Mendrysa, Susan M.; Kook, Hyun ⓘ Chonnam Natl Univ, Dept Pharmacol, Med Sch, Hwasun 58128, Jeollanamdo, South Korea; Chonnam Natl Univ, Basic Res Lab Vasc Remodeling, Med Sch, Hwasun 58128, Jeollanamdo, South Korea; Chonnam Natl Univ, BK21 Plus Ctr Creat Biomed Scientists, Gwangju, South Korea; Kyungpook Natl Univ, Coll Pharm, Daegu 41566, South Korea; Kyungpook Natl Univ, Res Inst Pharmaceut Sci, Daegu 41566, South Korea; Chonnam Natl Univ, Dept Anat, Med Sch, Hwasun 58128, Jeollanamdo, South Korea; Chonnam Natl Univ, Dept Forens Med, Med Sch, Hwasun 58128, Jeollanamdo, South Korea; Chonnam Natl Univ Hosp, Dept Cardiol, Gwangju 61469, South Korea; Chonnam Natl Univ, Dept Biochem, Med Sch, Hwasun 58128, Jeollanamdo, South Korea; Gwangju Inst Sci & Technol, Coll Life Sci, Gwangju 61005, South Korea; Purdue Univ, Coll Vet Med, Dept Basic Med Sci, W Lafayette, IN 47907 USA ⓘ Nam, Kwang Il/C-4580-2019; Kook, Hyun/AAR-5405-2021; Nam, Kwang/C-4580-2019; Kim, Young-Kook/AAE-8306-2020 ⓘ 54392133700; 15847530800; 14422691900; 57208255566; 7403396726; 13405024200; 7203003231; 57218341796; 56937721300; 57208862490; 14058468000; 6603479510; 7006255524 ⓘ elio9359@hanmail.net;kookhyun@chonnam.ac.kr; ⓘ EXPERIMENTAL AND MOLECULAR MEDICINE ⓘ EXP MOL MED ⓘ 1226-3613 2092-6413 53 11 SCIE BIOCHEMISTRY & MOLECULAR BIOLOGY;MEDICINE, RESEARCH & EXPERIMENTAL ⓘ 2021 12.172 6.9 0.4 2025-07-30 ⓘ 7 6 ⓘ SMOOTH-MUSCLE-CELLS; HOMEOBOX GENE; NEURAL CREST; OSTEOGENIC DIFFERENTIATION; EMBRYONIC LETHALITY; MDM2-DEFICIENT MICE; AORTIC STIFFNESS; MSX2 GENE; IN-VITRO; FAMILY ⓘ ⓘ Animals; Biomarkers; Calcium; Disease Models, Animal; Disease Susceptibility; Gene Expression Regulation; Gene Knockdown Techniques; Homeodomain Proteins; Humans; Male; Mice; Mice, Knockout; Models, Biological; MSX1 Transcription Factor; Mutation; Promoter Regions, Genetic; Proto-Oncogene Proteins c-mdm2; Response Elements; Ubiquitin-Protein Ligases; Vascular Calcification; colecalciferol; histone deacetylase 1; mouse double minute 2 homolog; transcription factor MSX1; transcription factor MSX2; ubiquitin protein ligase E3; biological marker; calcium; homeodomain protein; Mdm2 protein, mouse; mouse double minute 2 homolog; Msx1 protein, mouse; transcription factor MSX1; transcription factor MSX2; ubiquitin protein ligase; animal cell; animal experiment; animal model; Article; blood vessel calcification; controlled study; coronary artery calcification; enzyme activity; human; human tissue; in vivo study; male; molecular interaction; mouse; nonhuman; protein binding; protein function; regulatory mechanism; signal transduction; transcription initiation; transgenic mouse; animal; biological model; blood vessel calcification; disease model; disease predisposition; DNA responsive element; gene expression regulation; gene knockdown; genetics; knockout mouse; metabolism; mutation; pathology; promoter region ⓘ English 2021 2021-11 10.1038/s12276-021-00708-6 ⓘ 바로가기 바로가기 바로가기 바로가기
○ ○ Review ⓘ Trans-tail regulation-mediated suppression of cryptic transcription ⓘ Cell biology: Interaction of DNA-winding proteins ensures correct gene expression Crosstalk between different DNA-winding proteins, or histones, is a mechanism of molecular fidelity that helps prevent the initiation of aberrant gene expression, which may contribute to cancer and neurodegenerative disease. A team from South Korea, led by Jungmin Choi from the Korea University College of Medicine in Seoul and Hong-Yeoul Ryu from Kyungpook National University in Daegu, review the ways in which different histone proteins chemically modify parts of each other's structure to regulate their functions. These modifications affect how histones interact with DNA, which in turn alters the dynamics of other factors implicated in gene expression. The correct interaction of histones is necessary to prevent the gene expression machinery from starting RNA synthesis from the wrong sites. Accurate control of these mechanisms is essential for cellular wellbeing Crosstalk between post-translational modifications of histone proteins influences the regulation of chromatin structure and gene expression. Among such crosstalk pathways, the best-characterized example is H2B monoubiquitination-mediated H3K4 and H3K79 methylation, which is referred to as trans-tail regulation. Although many studies have investigated the fragmentary effects of this pathway on silencing and transcription, its ultimate contribution to transcriptional control has remained unclear. Recent advances in molecular techniques and genomics have, however, revealed that the trans-tail crosstalk is linked to a more diverse cascade of histone modifications and has various functions in cotranscriptional processes. Furthermore, H2B monoubiquitination sequentially facilitates H3K4 dimethylation and histone sumoylation, thereby providing a binding platform for recruiting Set3 complex proteins, including two histone deacetylases, to restrict cryptic transcription from gene bodies. The removal of both ubiquitin and SUMO, small ubiquitin-like modifier, modifications from histones also facilitates a change in the phosphorylation pattern of the RNA polymerase II C-terminal domain that is required for subsequent transcriptional elongation. Therefore, this review describes recent findings regarding trans-tail regulation-driven processes to elaborate on their contribution to maintaining transcriptional fidelity. ⓘ Choi, Jungmin; Ryoo, Zae Young; Cho, Dong-Hyung; Lee, Hyun-Shik; Ryu, Hong-Yeoul ⓘ Korea Univ, Dept Biomed Sci, Coll Med, Seoul 02841, South Korea; Kyungpook Natl Univ, Coll Natl Sci, Sch Life Sci, BK21 Four KNU Creat BioRes Grp, Daegu 41566, South Korea ⓘ RYOO, ZAEYOUNG/AAQ-1573-2020; Choi, Hye Rin/JDV-9065-2023 ⓘ 56589214500; 16937104900; 35093684400; 16316010200; 55889917800 ⓘ jungminchoi@korea.ac.kr;rhr4757@knu.ac.kr; ⓘ EXPERIMENTAL AND MOLECULAR MEDICINE ⓘ EXP MOL MED ⓘ 1226-3613 2092-6413 53 11 SCIE BIOCHEMISTRY & MOLECULAR BIOLOGY;MEDICINE, RESEARCH & EXPERIMENTAL ⓘ 2021 12.172 6.9 0.29 2025-07-30 ⓘ 9 9 ⓘ HISTONE H2B UBIQUITYLATION; RNA-POLYMERASE-II; H3 VARIANT CSE4; SYMMETRIC DIMETHYLATION; CHROMATIN MODIFICATIONS; SPURIOUS TRANSCRIPTION; DEACETYLASE COMPLEX; DNA METHYLATION; MONOUBIQUITINATION; ELONGATION ⓘ ⓘ Animals; Chromatin; Epigenesis, Genetic; Gene Expression Regulation; Gene Silencing; Histones; Humans; Methylation; Protein Processing, Post-Translational; Signal Transduction; Sumoylation; Transcription, Genetic; Ubiquitination; histone H2B; histone H3; histone; epigenetics; gene silencing; histone methylation; human; molecular interaction; nonhuman; protein function; regulatory mechanism; Review; Saccharomyces cerevisiae; sumoylation; transcription regulation; ubiquitination; animal; chromatin; gene expression regulation; genetic epigenesis; genetic transcription; genetics; metabolism; methylation; protein processing; signal transduction ⓘ English 2021 2021-11 10.1038/s12276-021-00711-x ⓘ 바로가기 바로가기 바로가기 바로가기
○ ○ Article ⓘ Advancements in Vehicular Communication Technologies: C-V2X and NR-V2X Comparison ⓘ Cellular vehicle-to-everything (C-V2X) is one of the key enabling vehicular communication technologies endorsed by the vehicular industry, scientists, and researchers. Introduced by the 3GPP, LTE device-to-device in Release 12 evolved into C-V2X with mode 3 and mode 4. In mode 4, semi-persistent -scheduling-based sensing is performed by vehicles to select resources. However, when the density of vehicles increases, the performance of C-V2X mode 4 degrades. To address this challenge, 3GPP Release 16 introduced New Radio (NR) for V2X services, also known as NR-V2X. Modes 1 and 2 along with four additional sub-modes were considered in NR-V2X to achieve high reliability and higher throughput with low latency. This article discusses and evaluates the advancements introduced in NR-V2X by comparative analysis with C-V2X. To compare the performance, an NR-V2X simulator based on a network simulator (ns-3) for sub-6 GHz band is also introduced. In addition, feasible practical solutions to resolve resource selection problems are also discussed. ⓘ Saad, Malik Muhammad; Khan, Muhammad Toaha Raza; Shah, Syed Hassan Ahmed; Kim, Dongkyun ⓘ Kyungpook Natl Univ, Sch Comp Sci & Engn, Daegu, South Korea; JMA Wireless, Syracuse, NY USA ⓘ Saad, Malik/ABF-9433-2021; Khan, Turyalai/HPH-0061-2023; Shah, Syed Hassan/E-5058-2014 ⓘ 57220715290; 57202044597; 55389144500; 35753648800 ⓘ maliksaad@knu.ac.kr;toaha@knu.ac.kr;sh.ahmed@ieee.org;dongkyun@knu.ac.kr; ⓘ IEEE COMMUNICATIONS MAGAZINE ⓘ IEEE COMMUN MAG ⓘ 0163-6804 1558-1896 59 8 SCIE ENGINEERING, ELECTRICAL & ELECTRONIC;TELECOMMUNICATIONS ⓘ 2021 9.03 7.0 4.65 2025-07-30 ⓘ 67 84 Performance evaluation; Long Term Evolution; Job shop scheduling; Vehicle-to-everything; New Radio; Sensors; 3GPP ⓘ PERFORMANCE ⓘ ⓘ Vehicles; Comparative analysis; GHz band; High reliability; Low latency; Network simulators; Practical solutions; Resource selection; Vehicular communication technologies; Mobile telecommunication systems ⓘ English 2021 2021-08 10.1109/mcom.101.2100119 ⓘ 바로가기 바로가기 바로가기 바로가기
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